Morphological description and molecular identification of Myxobolus dajiangensis n. sp. (Myxozoa: Myxobolidae) from the gill of Cyprinus carpio in southwest China
BACKGROUND: Myxosporean diversity is a hot topic since they are difficult to accurately identify and classify. Many Myxobolus parasites have been named as Myxobolus koi because of their similar morphological features with the species originally reported. However, the distinctions in fine morphologic...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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PeerJ Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8900608/ https://www.ncbi.nlm.nih.gov/pubmed/35265398 http://dx.doi.org/10.7717/peerj.13023 |
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author | Wang, Miao-miao Zhang, Jin-ye Zhao, Yuan-jun |
author_facet | Wang, Miao-miao Zhang, Jin-ye Zhao, Yuan-jun |
author_sort | Wang, Miao-miao |
collection | PubMed |
description | BACKGROUND: Myxosporean diversity is a hot topic since they are difficult to accurately identify and classify. Many Myxobolus parasites have been named as Myxobolus koi because of their similar morphological features with the species originally reported. However, the distinctions in fine morphological features, host specificity, and molecular data have given rise to the attention of researchers. METHODS: The classical morphometric and histological methods were used to describe the Myxobolus dajiangensis n. sp. in morphology. The common techniques in modern molecular biology and the methods of phylogenetic analyses were combined to identify the species. RESULTS: Plasmodia of interlamellar-vascular type were found in the vascular network of gill lamellae. Mature myxospores of M. dajiangensis n. sp. were elongated and pyriform from the frontal view. The myxospores were 14.8 ± 0.4 (13.9–15.6) µm in length, 8.0 ± 0.5 (7.2–9.1) µm in width, and 5.5 µm in thickness. The two polar capsules were pyriform and slightly different in length. The length of the larger polar capsules was 8.0 ± 0.4 (7.1–8.8) µm, and it was 7.4 ± 0.4 (6.1–8.0) µm for the smaller ones. The width of both polar capsules was 2.5 ± 0.2 (2.0–3.2) µm. The polar filaments within the polar capsules were each coiled nine to 11 turns. Comparative analysis of both the morphological and molecular data between the present speices and other similar species revealed that the present species is a novel species, Myxobolus dajiangensis n. sp. Also, M. koi (FJ710800) was misidentified and the congener with M. dajiangensis n. sp., depending on the secondary structures of SSU rRNA and phylogenetic analysis. Moreover, the cryptic species existed in the M. koi parasites. |
format | Online Article Text |
id | pubmed-8900608 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-89006082022-03-08 Morphological description and molecular identification of Myxobolus dajiangensis n. sp. (Myxozoa: Myxobolidae) from the gill of Cyprinus carpio in southwest China Wang, Miao-miao Zhang, Jin-ye Zhao, Yuan-jun PeerJ Aquaculture, Fisheries and Fish Science BACKGROUND: Myxosporean diversity is a hot topic since they are difficult to accurately identify and classify. Many Myxobolus parasites have been named as Myxobolus koi because of their similar morphological features with the species originally reported. However, the distinctions in fine morphological features, host specificity, and molecular data have given rise to the attention of researchers. METHODS: The classical morphometric and histological methods were used to describe the Myxobolus dajiangensis n. sp. in morphology. The common techniques in modern molecular biology and the methods of phylogenetic analyses were combined to identify the species. RESULTS: Plasmodia of interlamellar-vascular type were found in the vascular network of gill lamellae. Mature myxospores of M. dajiangensis n. sp. were elongated and pyriform from the frontal view. The myxospores were 14.8 ± 0.4 (13.9–15.6) µm in length, 8.0 ± 0.5 (7.2–9.1) µm in width, and 5.5 µm in thickness. The two polar capsules were pyriform and slightly different in length. The length of the larger polar capsules was 8.0 ± 0.4 (7.1–8.8) µm, and it was 7.4 ± 0.4 (6.1–8.0) µm for the smaller ones. The width of both polar capsules was 2.5 ± 0.2 (2.0–3.2) µm. The polar filaments within the polar capsules were each coiled nine to 11 turns. Comparative analysis of both the morphological and molecular data between the present speices and other similar species revealed that the present species is a novel species, Myxobolus dajiangensis n. sp. Also, M. koi (FJ710800) was misidentified and the congener with M. dajiangensis n. sp., depending on the secondary structures of SSU rRNA and phylogenetic analysis. Moreover, the cryptic species existed in the M. koi parasites. PeerJ Inc. 2022-03-04 /pmc/articles/PMC8900608/ /pubmed/35265398 http://dx.doi.org/10.7717/peerj.13023 Text en ©2022 Wang et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Aquaculture, Fisheries and Fish Science Wang, Miao-miao Zhang, Jin-ye Zhao, Yuan-jun Morphological description and molecular identification of Myxobolus dajiangensis n. sp. (Myxozoa: Myxobolidae) from the gill of Cyprinus carpio in southwest China |
title | Morphological description and molecular identification of Myxobolus dajiangensis n. sp. (Myxozoa: Myxobolidae) from the gill of Cyprinus carpio in southwest China |
title_full | Morphological description and molecular identification of Myxobolus dajiangensis n. sp. (Myxozoa: Myxobolidae) from the gill of Cyprinus carpio in southwest China |
title_fullStr | Morphological description and molecular identification of Myxobolus dajiangensis n. sp. (Myxozoa: Myxobolidae) from the gill of Cyprinus carpio in southwest China |
title_full_unstemmed | Morphological description and molecular identification of Myxobolus dajiangensis n. sp. (Myxozoa: Myxobolidae) from the gill of Cyprinus carpio in southwest China |
title_short | Morphological description and molecular identification of Myxobolus dajiangensis n. sp. (Myxozoa: Myxobolidae) from the gill of Cyprinus carpio in southwest China |
title_sort | morphological description and molecular identification of myxobolus dajiangensis n. sp. (myxozoa: myxobolidae) from the gill of cyprinus carpio in southwest china |
topic | Aquaculture, Fisheries and Fish Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8900608/ https://www.ncbi.nlm.nih.gov/pubmed/35265398 http://dx.doi.org/10.7717/peerj.13023 |
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