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A wide-field micro-computed tomography detector: micron resolution at half-centimetre scale

Ideal three-dimensional imaging of complex samples made up of micron-scale structures extending over mm to cm, such as biological tissues, requires both wide field of view and high resolution. For existing optics and detectors used for micro-CT (computed tomography) imaging, sub-micron pixel resolut...

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Autores principales: Yakovlev, Maksim A., Vanselow, Daniel J., Ngu, Mee Siing, Zaino, Carolyn R., Katz, Spencer R., Ding, Yifu, Parkinson, Dula, Wang, Steve Yuxin, Ang, Khai Chung, La Riviere, Patrick, Cheng, Keith C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8900834/
https://www.ncbi.nlm.nih.gov/pubmed/35254315
http://dx.doi.org/10.1107/S160057752101287X
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author Yakovlev, Maksim A.
Vanselow, Daniel J.
Ngu, Mee Siing
Zaino, Carolyn R.
Katz, Spencer R.
Ding, Yifu
Parkinson, Dula
Wang, Steve Yuxin
Ang, Khai Chung
La Riviere, Patrick
Cheng, Keith C.
author_facet Yakovlev, Maksim A.
Vanselow, Daniel J.
Ngu, Mee Siing
Zaino, Carolyn R.
Katz, Spencer R.
Ding, Yifu
Parkinson, Dula
Wang, Steve Yuxin
Ang, Khai Chung
La Riviere, Patrick
Cheng, Keith C.
author_sort Yakovlev, Maksim A.
collection PubMed
description Ideal three-dimensional imaging of complex samples made up of micron-scale structures extending over mm to cm, such as biological tissues, requires both wide field of view and high resolution. For existing optics and detectors used for micro-CT (computed tomography) imaging, sub-micron pixel resolution can only be achieved for fields of view of <2 mm. This article presents a unique detector system with a 6 mm field-of-view image circle and 0.5 µm pixel size that can be used in micro-CT units utilizing both synchrotron and commercial X-ray sources. A resolution-test pattern with linear microstructures and whole adult Daphnia magna were imaged at beamline 8.3.2 of the Berkeley Advanced Light Source. Volumes of 10000 × 10000 × 7096 isotropic 0.5 µm voxels were reconstructed over a 5.0 mm × 3.5 mm field of view. Measurements in the projection domain confirmed a 0.90 µm measured spatial resolution that is largely Nyquist-limited. This unprecedented combination of field of view and resolution dramatically reduces the need for sectional scans and computational stitching for large samples, ultimately offering the means to elucidate changes in tissue and cellular morphology in the context of larger, whole, intact model organisms and specimens. This system is also anticipated to benefit micro-CT imaging in materials science, microelectronics, agricultural science and biomedical engineering.
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spelling pubmed-89008342022-03-29 A wide-field micro-computed tomography detector: micron resolution at half-centimetre scale Yakovlev, Maksim A. Vanselow, Daniel J. Ngu, Mee Siing Zaino, Carolyn R. Katz, Spencer R. Ding, Yifu Parkinson, Dula Wang, Steve Yuxin Ang, Khai Chung La Riviere, Patrick Cheng, Keith C. J Synchrotron Radiat Research Papers Ideal three-dimensional imaging of complex samples made up of micron-scale structures extending over mm to cm, such as biological tissues, requires both wide field of view and high resolution. For existing optics and detectors used for micro-CT (computed tomography) imaging, sub-micron pixel resolution can only be achieved for fields of view of <2 mm. This article presents a unique detector system with a 6 mm field-of-view image circle and 0.5 µm pixel size that can be used in micro-CT units utilizing both synchrotron and commercial X-ray sources. A resolution-test pattern with linear microstructures and whole adult Daphnia magna were imaged at beamline 8.3.2 of the Berkeley Advanced Light Source. Volumes of 10000 × 10000 × 7096 isotropic 0.5 µm voxels were reconstructed over a 5.0 mm × 3.5 mm field of view. Measurements in the projection domain confirmed a 0.90 µm measured spatial resolution that is largely Nyquist-limited. This unprecedented combination of field of view and resolution dramatically reduces the need for sectional scans and computational stitching for large samples, ultimately offering the means to elucidate changes in tissue and cellular morphology in the context of larger, whole, intact model organisms and specimens. This system is also anticipated to benefit micro-CT imaging in materials science, microelectronics, agricultural science and biomedical engineering. International Union of Crystallography 2022-01-19 /pmc/articles/PMC8900834/ /pubmed/35254315 http://dx.doi.org/10.1107/S160057752101287X Text en © Maksim A. Yakovlev et al. 2022 https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Research Papers
Yakovlev, Maksim A.
Vanselow, Daniel J.
Ngu, Mee Siing
Zaino, Carolyn R.
Katz, Spencer R.
Ding, Yifu
Parkinson, Dula
Wang, Steve Yuxin
Ang, Khai Chung
La Riviere, Patrick
Cheng, Keith C.
A wide-field micro-computed tomography detector: micron resolution at half-centimetre scale
title A wide-field micro-computed tomography detector: micron resolution at half-centimetre scale
title_full A wide-field micro-computed tomography detector: micron resolution at half-centimetre scale
title_fullStr A wide-field micro-computed tomography detector: micron resolution at half-centimetre scale
title_full_unstemmed A wide-field micro-computed tomography detector: micron resolution at half-centimetre scale
title_short A wide-field micro-computed tomography detector: micron resolution at half-centimetre scale
title_sort wide-field micro-computed tomography detector: micron resolution at half-centimetre scale
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8900834/
https://www.ncbi.nlm.nih.gov/pubmed/35254315
http://dx.doi.org/10.1107/S160057752101287X
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