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A wide-field micro-computed tomography detector: micron resolution at half-centimetre scale
Ideal three-dimensional imaging of complex samples made up of micron-scale structures extending over mm to cm, such as biological tissues, requires both wide field of view and high resolution. For existing optics and detectors used for micro-CT (computed tomography) imaging, sub-micron pixel resolut...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Union of Crystallography
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8900834/ https://www.ncbi.nlm.nih.gov/pubmed/35254315 http://dx.doi.org/10.1107/S160057752101287X |
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author | Yakovlev, Maksim A. Vanselow, Daniel J. Ngu, Mee Siing Zaino, Carolyn R. Katz, Spencer R. Ding, Yifu Parkinson, Dula Wang, Steve Yuxin Ang, Khai Chung La Riviere, Patrick Cheng, Keith C. |
author_facet | Yakovlev, Maksim A. Vanselow, Daniel J. Ngu, Mee Siing Zaino, Carolyn R. Katz, Spencer R. Ding, Yifu Parkinson, Dula Wang, Steve Yuxin Ang, Khai Chung La Riviere, Patrick Cheng, Keith C. |
author_sort | Yakovlev, Maksim A. |
collection | PubMed |
description | Ideal three-dimensional imaging of complex samples made up of micron-scale structures extending over mm to cm, such as biological tissues, requires both wide field of view and high resolution. For existing optics and detectors used for micro-CT (computed tomography) imaging, sub-micron pixel resolution can only be achieved for fields of view of <2 mm. This article presents a unique detector system with a 6 mm field-of-view image circle and 0.5 µm pixel size that can be used in micro-CT units utilizing both synchrotron and commercial X-ray sources. A resolution-test pattern with linear microstructures and whole adult Daphnia magna were imaged at beamline 8.3.2 of the Berkeley Advanced Light Source. Volumes of 10000 × 10000 × 7096 isotropic 0.5 µm voxels were reconstructed over a 5.0 mm × 3.5 mm field of view. Measurements in the projection domain confirmed a 0.90 µm measured spatial resolution that is largely Nyquist-limited. This unprecedented combination of field of view and resolution dramatically reduces the need for sectional scans and computational stitching for large samples, ultimately offering the means to elucidate changes in tissue and cellular morphology in the context of larger, whole, intact model organisms and specimens. This system is also anticipated to benefit micro-CT imaging in materials science, microelectronics, agricultural science and biomedical engineering. |
format | Online Article Text |
id | pubmed-8900834 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | International Union of Crystallography |
record_format | MEDLINE/PubMed |
spelling | pubmed-89008342022-03-29 A wide-field micro-computed tomography detector: micron resolution at half-centimetre scale Yakovlev, Maksim A. Vanselow, Daniel J. Ngu, Mee Siing Zaino, Carolyn R. Katz, Spencer R. Ding, Yifu Parkinson, Dula Wang, Steve Yuxin Ang, Khai Chung La Riviere, Patrick Cheng, Keith C. J Synchrotron Radiat Research Papers Ideal three-dimensional imaging of complex samples made up of micron-scale structures extending over mm to cm, such as biological tissues, requires both wide field of view and high resolution. For existing optics and detectors used for micro-CT (computed tomography) imaging, sub-micron pixel resolution can only be achieved for fields of view of <2 mm. This article presents a unique detector system with a 6 mm field-of-view image circle and 0.5 µm pixel size that can be used in micro-CT units utilizing both synchrotron and commercial X-ray sources. A resolution-test pattern with linear microstructures and whole adult Daphnia magna were imaged at beamline 8.3.2 of the Berkeley Advanced Light Source. Volumes of 10000 × 10000 × 7096 isotropic 0.5 µm voxels were reconstructed over a 5.0 mm × 3.5 mm field of view. Measurements in the projection domain confirmed a 0.90 µm measured spatial resolution that is largely Nyquist-limited. This unprecedented combination of field of view and resolution dramatically reduces the need for sectional scans and computational stitching for large samples, ultimately offering the means to elucidate changes in tissue and cellular morphology in the context of larger, whole, intact model organisms and specimens. This system is also anticipated to benefit micro-CT imaging in materials science, microelectronics, agricultural science and biomedical engineering. International Union of Crystallography 2022-01-19 /pmc/articles/PMC8900834/ /pubmed/35254315 http://dx.doi.org/10.1107/S160057752101287X Text en © Maksim A. Yakovlev et al. 2022 https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited. |
spellingShingle | Research Papers Yakovlev, Maksim A. Vanselow, Daniel J. Ngu, Mee Siing Zaino, Carolyn R. Katz, Spencer R. Ding, Yifu Parkinson, Dula Wang, Steve Yuxin Ang, Khai Chung La Riviere, Patrick Cheng, Keith C. A wide-field micro-computed tomography detector: micron resolution at half-centimetre scale |
title | A wide-field micro-computed tomography detector: micron resolution at half-centimetre scale |
title_full | A wide-field micro-computed tomography detector: micron resolution at half-centimetre scale |
title_fullStr | A wide-field micro-computed tomography detector: micron resolution at half-centimetre scale |
title_full_unstemmed | A wide-field micro-computed tomography detector: micron resolution at half-centimetre scale |
title_short | A wide-field micro-computed tomography detector: micron resolution at half-centimetre scale |
title_sort | wide-field micro-computed tomography detector: micron resolution at half-centimetre scale |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8900834/ https://www.ncbi.nlm.nih.gov/pubmed/35254315 http://dx.doi.org/10.1107/S160057752101287X |
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