An Overview of SARS-CoV-2 Molecular Diagnostics in Europe

The COVID-19 pandemic has led to the rapid development of a plethora of molecular diagnostic assays with real-time polymerase chain reaction (RT-PCR) at the forefront. In this review, we will discuss the history and utility of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) molecular di...

Descripción completa

Detalles Bibliográficos
Autores principales: Davies, Emma, Farooq, Hamzah Z., Brown, Benjamin, Tilston, Peter, McEwan, Ashley, Birtles, Andrew, O’Hara, Robert William, Ahmad, Shazaad, Machin, Nicholas, Hesketh, Louise, Guiver, Malcolm
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier Inc. 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8901364/
https://www.ncbi.nlm.nih.gov/pubmed/35636820
http://dx.doi.org/10.1016/j.cll.2022.02.005
_version_ 1784664358577504256
author Davies, Emma
Farooq, Hamzah Z.
Brown, Benjamin
Tilston, Peter
McEwan, Ashley
Birtles, Andrew
O’Hara, Robert William
Ahmad, Shazaad
Machin, Nicholas
Hesketh, Louise
Guiver, Malcolm
author_facet Davies, Emma
Farooq, Hamzah Z.
Brown, Benjamin
Tilston, Peter
McEwan, Ashley
Birtles, Andrew
O’Hara, Robert William
Ahmad, Shazaad
Machin, Nicholas
Hesketh, Louise
Guiver, Malcolm
author_sort Davies, Emma
collection PubMed
description The COVID-19 pandemic has led to the rapid development of a plethora of molecular diagnostic assays with real-time polymerase chain reaction (RT-PCR) at the forefront. In this review, we will discuss the history and utility of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) molecular diagnostics and the associated current and future regulatory process in Europe. We will assess the performance characteristics of a range of the most common SARS-CoV-2 molecular tests currently used in Europe with a focus on as rapid molecular platforms, stand-alone RT-PCR kits, the role of low-throughput and high-throughput end-to-end testing platforms, and the rapidly evolving field of SARS-CoV-2 variant of concern identification.
format Online
Article
Text
id pubmed-8901364
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Published by Elsevier Inc.
record_format MEDLINE/PubMed
spelling pubmed-89013642022-03-08 An Overview of SARS-CoV-2 Molecular Diagnostics in Europe Davies, Emma Farooq, Hamzah Z. Brown, Benjamin Tilston, Peter McEwan, Ashley Birtles, Andrew O’Hara, Robert William Ahmad, Shazaad Machin, Nicholas Hesketh, Louise Guiver, Malcolm Clin Lab Med Article The COVID-19 pandemic has led to the rapid development of a plethora of molecular diagnostic assays with real-time polymerase chain reaction (RT-PCR) at the forefront. In this review, we will discuss the history and utility of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) molecular diagnostics and the associated current and future regulatory process in Europe. We will assess the performance characteristics of a range of the most common SARS-CoV-2 molecular tests currently used in Europe with a focus on as rapid molecular platforms, stand-alone RT-PCR kits, the role of low-throughput and high-throughput end-to-end testing platforms, and the rapidly evolving field of SARS-CoV-2 variant of concern identification. Published by Elsevier Inc. 2022-06 2022-03-08 /pmc/articles/PMC8901364/ /pubmed/35636820 http://dx.doi.org/10.1016/j.cll.2022.02.005 Text en Crown Copyright © 2022 Published by Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Davies, Emma
Farooq, Hamzah Z.
Brown, Benjamin
Tilston, Peter
McEwan, Ashley
Birtles, Andrew
O’Hara, Robert William
Ahmad, Shazaad
Machin, Nicholas
Hesketh, Louise
Guiver, Malcolm
An Overview of SARS-CoV-2 Molecular Diagnostics in Europe
title An Overview of SARS-CoV-2 Molecular Diagnostics in Europe
title_full An Overview of SARS-CoV-2 Molecular Diagnostics in Europe
title_fullStr An Overview of SARS-CoV-2 Molecular Diagnostics in Europe
title_full_unstemmed An Overview of SARS-CoV-2 Molecular Diagnostics in Europe
title_short An Overview of SARS-CoV-2 Molecular Diagnostics in Europe
title_sort overview of sars-cov-2 molecular diagnostics in europe
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8901364/
https://www.ncbi.nlm.nih.gov/pubmed/35636820
http://dx.doi.org/10.1016/j.cll.2022.02.005
work_keys_str_mv AT daviesemma anoverviewofsarscov2moleculardiagnosticsineurope
AT farooqhamzahz anoverviewofsarscov2moleculardiagnosticsineurope
AT brownbenjamin anoverviewofsarscov2moleculardiagnosticsineurope
AT tilstonpeter anoverviewofsarscov2moleculardiagnosticsineurope
AT mcewanashley anoverviewofsarscov2moleculardiagnosticsineurope
AT birtlesandrew anoverviewofsarscov2moleculardiagnosticsineurope
AT ohararobertwilliam anoverviewofsarscov2moleculardiagnosticsineurope
AT ahmadshazaad anoverviewofsarscov2moleculardiagnosticsineurope
AT machinnicholas anoverviewofsarscov2moleculardiagnosticsineurope
AT heskethlouise anoverviewofsarscov2moleculardiagnosticsineurope
AT guivermalcolm anoverviewofsarscov2moleculardiagnosticsineurope
AT daviesemma overviewofsarscov2moleculardiagnosticsineurope
AT farooqhamzahz overviewofsarscov2moleculardiagnosticsineurope
AT brownbenjamin overviewofsarscov2moleculardiagnosticsineurope
AT tilstonpeter overviewofsarscov2moleculardiagnosticsineurope
AT mcewanashley overviewofsarscov2moleculardiagnosticsineurope
AT birtlesandrew overviewofsarscov2moleculardiagnosticsineurope
AT ohararobertwilliam overviewofsarscov2moleculardiagnosticsineurope
AT ahmadshazaad overviewofsarscov2moleculardiagnosticsineurope
AT machinnicholas overviewofsarscov2moleculardiagnosticsineurope
AT heskethlouise overviewofsarscov2moleculardiagnosticsineurope
AT guivermalcolm overviewofsarscov2moleculardiagnosticsineurope

Ejemplares similares