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8C-like cells capture the human zygotic genome activation program in vitro
The activation of the embryonic genome marks the first major wave of transcription in the developing organism. Zygotic genome activation (ZGA) in mouse 2-cell embryos and 8-cell embryos in humans is crucial for development. Here, we report the discovery of human 8-cell-like cells (8CLCs) among naive...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cell Press
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8901440/ https://www.ncbi.nlm.nih.gov/pubmed/35216671 http://dx.doi.org/10.1016/j.stem.2022.01.014 |
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author | Taubenschmid-Stowers, Jasmin Rostovskaya, Maria Santos, Fátima Ljung, Sebastian Argelaguet, Ricard Krueger, Felix Nichols, Jennifer Reik, Wolf |
author_facet | Taubenschmid-Stowers, Jasmin Rostovskaya, Maria Santos, Fátima Ljung, Sebastian Argelaguet, Ricard Krueger, Felix Nichols, Jennifer Reik, Wolf |
author_sort | Taubenschmid-Stowers, Jasmin |
collection | PubMed |
description | The activation of the embryonic genome marks the first major wave of transcription in the developing organism. Zygotic genome activation (ZGA) in mouse 2-cell embryos and 8-cell embryos in humans is crucial for development. Here, we report the discovery of human 8-cell-like cells (8CLCs) among naive embryonic stem cells, which transcriptionally resemble the 8-cell human embryo. They express ZGA markers, including ZSCAN4 and LEUTX, and transposable elements, such as HERVL and MLT2A1. 8CLCs show reduced SOX2 levels and can be identified using TPRX1 and H3.Y marker proteins in vitro. Overexpression of the transcription factor DUX4 and spliceosome inhibition increase human ZGA-like transcription. Excitingly, the 8CLC markers TPRX1 and H3.Y are also expressed in ZGA-stage 8-cell human embryos and may thus be relevant in vivo. 8CLCs provide a unique opportunity to characterize human ZGA-like transcription and might provide critical insights into early events in embryogenesis in humans. |
format | Online Article Text |
id | pubmed-8901440 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Cell Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-89014402022-03-11 8C-like cells capture the human zygotic genome activation program in vitro Taubenschmid-Stowers, Jasmin Rostovskaya, Maria Santos, Fátima Ljung, Sebastian Argelaguet, Ricard Krueger, Felix Nichols, Jennifer Reik, Wolf Cell Stem Cell Short Article The activation of the embryonic genome marks the first major wave of transcription in the developing organism. Zygotic genome activation (ZGA) in mouse 2-cell embryos and 8-cell embryos in humans is crucial for development. Here, we report the discovery of human 8-cell-like cells (8CLCs) among naive embryonic stem cells, which transcriptionally resemble the 8-cell human embryo. They express ZGA markers, including ZSCAN4 and LEUTX, and transposable elements, such as HERVL and MLT2A1. 8CLCs show reduced SOX2 levels and can be identified using TPRX1 and H3.Y marker proteins in vitro. Overexpression of the transcription factor DUX4 and spliceosome inhibition increase human ZGA-like transcription. Excitingly, the 8CLC markers TPRX1 and H3.Y are also expressed in ZGA-stage 8-cell human embryos and may thus be relevant in vivo. 8CLCs provide a unique opportunity to characterize human ZGA-like transcription and might provide critical insights into early events in embryogenesis in humans. Cell Press 2022-03-03 /pmc/articles/PMC8901440/ /pubmed/35216671 http://dx.doi.org/10.1016/j.stem.2022.01.014 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Short Article Taubenschmid-Stowers, Jasmin Rostovskaya, Maria Santos, Fátima Ljung, Sebastian Argelaguet, Ricard Krueger, Felix Nichols, Jennifer Reik, Wolf 8C-like cells capture the human zygotic genome activation program in vitro |
title | 8C-like cells capture the human zygotic genome activation program in vitro |
title_full | 8C-like cells capture the human zygotic genome activation program in vitro |
title_fullStr | 8C-like cells capture the human zygotic genome activation program in vitro |
title_full_unstemmed | 8C-like cells capture the human zygotic genome activation program in vitro |
title_short | 8C-like cells capture the human zygotic genome activation program in vitro |
title_sort | 8c-like cells capture the human zygotic genome activation program in vitro |
topic | Short Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8901440/ https://www.ncbi.nlm.nih.gov/pubmed/35216671 http://dx.doi.org/10.1016/j.stem.2022.01.014 |
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