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8C-like cells capture the human zygotic genome activation program in vitro

The activation of the embryonic genome marks the first major wave of transcription in the developing organism. Zygotic genome activation (ZGA) in mouse 2-cell embryos and 8-cell embryos in humans is crucial for development. Here, we report the discovery of human 8-cell-like cells (8CLCs) among naive...

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Autores principales: Taubenschmid-Stowers, Jasmin, Rostovskaya, Maria, Santos, Fátima, Ljung, Sebastian, Argelaguet, Ricard, Krueger, Felix, Nichols, Jennifer, Reik, Wolf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cell Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8901440/
https://www.ncbi.nlm.nih.gov/pubmed/35216671
http://dx.doi.org/10.1016/j.stem.2022.01.014
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author Taubenschmid-Stowers, Jasmin
Rostovskaya, Maria
Santos, Fátima
Ljung, Sebastian
Argelaguet, Ricard
Krueger, Felix
Nichols, Jennifer
Reik, Wolf
author_facet Taubenschmid-Stowers, Jasmin
Rostovskaya, Maria
Santos, Fátima
Ljung, Sebastian
Argelaguet, Ricard
Krueger, Felix
Nichols, Jennifer
Reik, Wolf
author_sort Taubenschmid-Stowers, Jasmin
collection PubMed
description The activation of the embryonic genome marks the first major wave of transcription in the developing organism. Zygotic genome activation (ZGA) in mouse 2-cell embryos and 8-cell embryos in humans is crucial for development. Here, we report the discovery of human 8-cell-like cells (8CLCs) among naive embryonic stem cells, which transcriptionally resemble the 8-cell human embryo. They express ZGA markers, including ZSCAN4 and LEUTX, and transposable elements, such as HERVL and MLT2A1. 8CLCs show reduced SOX2 levels and can be identified using TPRX1 and H3.Y marker proteins in vitro. Overexpression of the transcription factor DUX4 and spliceosome inhibition increase human ZGA-like transcription. Excitingly, the 8CLC markers TPRX1 and H3.Y are also expressed in ZGA-stage 8-cell human embryos and may thus be relevant in vivo. 8CLCs provide a unique opportunity to characterize human ZGA-like transcription and might provide critical insights into early events in embryogenesis in humans.
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spelling pubmed-89014402022-03-11 8C-like cells capture the human zygotic genome activation program in vitro Taubenschmid-Stowers, Jasmin Rostovskaya, Maria Santos, Fátima Ljung, Sebastian Argelaguet, Ricard Krueger, Felix Nichols, Jennifer Reik, Wolf Cell Stem Cell Short Article The activation of the embryonic genome marks the first major wave of transcription in the developing organism. Zygotic genome activation (ZGA) in mouse 2-cell embryos and 8-cell embryos in humans is crucial for development. Here, we report the discovery of human 8-cell-like cells (8CLCs) among naive embryonic stem cells, which transcriptionally resemble the 8-cell human embryo. They express ZGA markers, including ZSCAN4 and LEUTX, and transposable elements, such as HERVL and MLT2A1. 8CLCs show reduced SOX2 levels and can be identified using TPRX1 and H3.Y marker proteins in vitro. Overexpression of the transcription factor DUX4 and spliceosome inhibition increase human ZGA-like transcription. Excitingly, the 8CLC markers TPRX1 and H3.Y are also expressed in ZGA-stage 8-cell human embryos and may thus be relevant in vivo. 8CLCs provide a unique opportunity to characterize human ZGA-like transcription and might provide critical insights into early events in embryogenesis in humans. Cell Press 2022-03-03 /pmc/articles/PMC8901440/ /pubmed/35216671 http://dx.doi.org/10.1016/j.stem.2022.01.014 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Short Article
Taubenschmid-Stowers, Jasmin
Rostovskaya, Maria
Santos, Fátima
Ljung, Sebastian
Argelaguet, Ricard
Krueger, Felix
Nichols, Jennifer
Reik, Wolf
8C-like cells capture the human zygotic genome activation program in vitro
title 8C-like cells capture the human zygotic genome activation program in vitro
title_full 8C-like cells capture the human zygotic genome activation program in vitro
title_fullStr 8C-like cells capture the human zygotic genome activation program in vitro
title_full_unstemmed 8C-like cells capture the human zygotic genome activation program in vitro
title_short 8C-like cells capture the human zygotic genome activation program in vitro
title_sort 8c-like cells capture the human zygotic genome activation program in vitro
topic Short Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8901440/
https://www.ncbi.nlm.nih.gov/pubmed/35216671
http://dx.doi.org/10.1016/j.stem.2022.01.014
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