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Single 3′-exonuclease-based multifragment DNA assembly method (SENAX)

DNA assembly is a vital process in biotechnology and synthetic biology research, during which DNA plasmids are designed and constructed using bioparts to engineer microorganisms for a wide range of applications. Here, we present an enzymatic homology-based DNA assembly method, SENAX (Stellar ExoNucl...

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Detalles Bibliográficos
Autores principales: Dao, Viet Linh, Chan, Sheena, Zhang, Jingyun, Ngo, Russell Kai Jie, Poh, Chueh Loo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8901738/
https://www.ncbi.nlm.nih.gov/pubmed/35256704
http://dx.doi.org/10.1038/s41598-022-07878-x