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Development of a minimally invasive pulmonary porcine embolism model via endobronchial ultrasound

BACKGROUND: Current massive pulmonary embolism (PE) animal models use central venous access to deliver blood clots, which have features of random clot distribution and potentially fatal hemodynamic compromise. A clinically relevant preclinical model for generating pulmonary emboli in a more controll...

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Autores principales: Inage, Terunaga, Fujino, Kosuke, Motooka, Yamato, Ishiwata, Tsukasa, Ujiie, Hideki, Gregor, Alexander, Bernards, Nicholas, Chan, Harley H. L., Chen, Zhenchian, Aragaki, Masato, Kinoshita, Tomonari, Effat, Andrew, Yoshino, Ichiro, Yasufuku, Kazuhiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AME Publishing Company 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8902127/
https://www.ncbi.nlm.nih.gov/pubmed/35280485
http://dx.doi.org/10.21037/jtd-21-1242
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author Inage, Terunaga
Fujino, Kosuke
Motooka, Yamato
Ishiwata, Tsukasa
Ujiie, Hideki
Gregor, Alexander
Bernards, Nicholas
Chan, Harley H. L.
Chen, Zhenchian
Aragaki, Masato
Kinoshita, Tomonari
Effat, Andrew
Yoshino, Ichiro
Yasufuku, Kazuhiro
author_facet Inage, Terunaga
Fujino, Kosuke
Motooka, Yamato
Ishiwata, Tsukasa
Ujiie, Hideki
Gregor, Alexander
Bernards, Nicholas
Chan, Harley H. L.
Chen, Zhenchian
Aragaki, Masato
Kinoshita, Tomonari
Effat, Andrew
Yoshino, Ichiro
Yasufuku, Kazuhiro
author_sort Inage, Terunaga
collection PubMed
description BACKGROUND: Current massive pulmonary embolism (PE) animal models use central venous access to deliver blood clots, which have features of random clot distribution and potentially fatal hemodynamic compromise. A clinically relevant preclinical model for generating pulmonary emboli in a more controlled fashion would be of value for a variety of research studies, including initial evaluation of novel therapeutic approaches. Endobronchial ultrasound-guided transbronchial needle injection (EBUS-TBNI) is a newly established approach for peri-tracheal/bronchial targets. The purpose of the present work was to establish a minimally invasive PE model in swine via a transbronchial approach. METHODS: In anesthetized Yorkshire pigs, a 21-G EBUS-guided transbronchial needle aspiration (EBUS-TBNA) needle was introduced into the pulmonary artery under EBUS guidance. Autologous blood clots were administered into the right and left lower pulmonary arteries sequentially (PE1 and PE2, respectively). Hemodynamic and biochemical responses were evaluated. RESULTS: Ten pigs were evaluated; all 20 blood clots (6.3±1.9 mL) were successfully injected. After injection, mean pulmonary artery pressure (mPAP; mmHg) increased (baseline: 16.6±5.6 vs. PE1: 24.5±7.6, P<0.0001 vs. PE2: 26.9±6.7, P<0.0001), and a positive correlation was observed between clot volume and change in mPAP (PE1: r=0.69, P=0.025; PE1 + PE2: r=0.60, P=0.063). Mean arterial pressure (MAP; mmHg) (baseline: 57.5±5.1 vs. PE1: 59.0±9.1, P=0.918 vs. PE2: 60.9±9.6, P=0.664) remained stable. No complications were observed. CONCLUSIONS: EBUS allows minimally invasive, precise, and reliable generation of pulmonary emboli in pigs. This model may serve as an important tool for new PE-related diagnostic and therapeutic research.
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spelling pubmed-89021272022-03-10 Development of a minimally invasive pulmonary porcine embolism model via endobronchial ultrasound Inage, Terunaga Fujino, Kosuke Motooka, Yamato Ishiwata, Tsukasa Ujiie, Hideki Gregor, Alexander Bernards, Nicholas Chan, Harley H. L. Chen, Zhenchian Aragaki, Masato Kinoshita, Tomonari Effat, Andrew Yoshino, Ichiro Yasufuku, Kazuhiro J Thorac Dis Original Article BACKGROUND: Current massive pulmonary embolism (PE) animal models use central venous access to deliver blood clots, which have features of random clot distribution and potentially fatal hemodynamic compromise. A clinically relevant preclinical model for generating pulmonary emboli in a more controlled fashion would be of value for a variety of research studies, including initial evaluation of novel therapeutic approaches. Endobronchial ultrasound-guided transbronchial needle injection (EBUS-TBNI) is a newly established approach for peri-tracheal/bronchial targets. The purpose of the present work was to establish a minimally invasive PE model in swine via a transbronchial approach. METHODS: In anesthetized Yorkshire pigs, a 21-G EBUS-guided transbronchial needle aspiration (EBUS-TBNA) needle was introduced into the pulmonary artery under EBUS guidance. Autologous blood clots were administered into the right and left lower pulmonary arteries sequentially (PE1 and PE2, respectively). Hemodynamic and biochemical responses were evaluated. RESULTS: Ten pigs were evaluated; all 20 blood clots (6.3±1.9 mL) were successfully injected. After injection, mean pulmonary artery pressure (mPAP; mmHg) increased (baseline: 16.6±5.6 vs. PE1: 24.5±7.6, P<0.0001 vs. PE2: 26.9±6.7, P<0.0001), and a positive correlation was observed between clot volume and change in mPAP (PE1: r=0.69, P=0.025; PE1 + PE2: r=0.60, P=0.063). Mean arterial pressure (MAP; mmHg) (baseline: 57.5±5.1 vs. PE1: 59.0±9.1, P=0.918 vs. PE2: 60.9±9.6, P=0.664) remained stable. No complications were observed. CONCLUSIONS: EBUS allows minimally invasive, precise, and reliable generation of pulmonary emboli in pigs. This model may serve as an important tool for new PE-related diagnostic and therapeutic research. AME Publishing Company 2022-02 /pmc/articles/PMC8902127/ /pubmed/35280485 http://dx.doi.org/10.21037/jtd-21-1242 Text en 2022 Journal of Thoracic Disease. All rights reserved. https://creativecommons.org/licenses/by-nc-nd/4.0/Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Original Article
Inage, Terunaga
Fujino, Kosuke
Motooka, Yamato
Ishiwata, Tsukasa
Ujiie, Hideki
Gregor, Alexander
Bernards, Nicholas
Chan, Harley H. L.
Chen, Zhenchian
Aragaki, Masato
Kinoshita, Tomonari
Effat, Andrew
Yoshino, Ichiro
Yasufuku, Kazuhiro
Development of a minimally invasive pulmonary porcine embolism model via endobronchial ultrasound
title Development of a minimally invasive pulmonary porcine embolism model via endobronchial ultrasound
title_full Development of a minimally invasive pulmonary porcine embolism model via endobronchial ultrasound
title_fullStr Development of a minimally invasive pulmonary porcine embolism model via endobronchial ultrasound
title_full_unstemmed Development of a minimally invasive pulmonary porcine embolism model via endobronchial ultrasound
title_short Development of a minimally invasive pulmonary porcine embolism model via endobronchial ultrasound
title_sort development of a minimally invasive pulmonary porcine embolism model via endobronchial ultrasound
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8902127/
https://www.ncbi.nlm.nih.gov/pubmed/35280485
http://dx.doi.org/10.21037/jtd-21-1242
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