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Screening and Verification of Differentially Expressed Long Non-coding RNAs in the Peripheral Blood of Patients With asthma

Growing evidence suggests that long non-coding RNAs (lncRNAs) play a key role in the pathogenesis of asthma. Although some differentially expressed lncRNAs have been identified in asthmatic patients, many asthma-related lncRNAs have not been annotated. In the present study, six patients and three he...

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Autores principales: Ma, Cheng, Wang, Shiyuan, Cao, Yuxue, Tang, Weifeng, Wuniqiemu, Tulake, Teng, Fangzhou, Zhu, Xueyi, Wei, Ying, Dong, Jingcheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8902465/
https://www.ncbi.nlm.nih.gov/pubmed/35273507
http://dx.doi.org/10.3389/fphar.2022.834009
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author Ma, Cheng
Wang, Shiyuan
Cao, Yuxue
Tang, Weifeng
Wuniqiemu, Tulake
Teng, Fangzhou
Zhu, Xueyi
Wei, Ying
Dong, Jingcheng
author_facet Ma, Cheng
Wang, Shiyuan
Cao, Yuxue
Tang, Weifeng
Wuniqiemu, Tulake
Teng, Fangzhou
Zhu, Xueyi
Wei, Ying
Dong, Jingcheng
author_sort Ma, Cheng
collection PubMed
description Growing evidence suggests that long non-coding RNAs (lncRNAs) play a key role in the pathogenesis of asthma. Although some differentially expressed lncRNAs have been identified in asthmatic patients, many asthma-related lncRNAs have not been annotated. In the present study, six patients and three healthy subjects were randomly selected from 34 asthmatic patients and 17 healthy subjects. Second-generation high-throughput sequencing was performed on their peripheral blood samples. There were 1,137 differentially expressed lncRNAs in the asthma patients compared to in the healthy controls, of which 485 were upregulated and 652 were downregulated. The top 30 enriched GO and KEGG terms were identified, and the cytosolic ribosome (GO:0022626) and ribosome (hsa03010) were associated with the most differentially expressed lncRNAs. The top 10 differentially expressed lncRNAs associated with asthma were verified by an lncRNA-mRNA co-expression network and RT-qPCR. Seven of the these (NONHSAT015495.2, MSTRG.71212.2, NONHSAT163272.1, NONHSAT181891.1, NONHSAT190964.1, ENST00000564809, and NONHSAT076890.2) were down-regulated in the peripheral blood of asthmatic patients, which was consistent with the sequencing results. Three patients and three healthy subjects were randomly selected from the remaining subjects to verify these seven lncRNAs by RT-qPCR, which further confirmed the sequencing results. Public database GSE106230 was also in agreement with the FPKM (Fragments Per kilobase of exon model per Million mapped reads) trends of ENST00000564809, NONHSAT015495.2, NONHSAT181891.1, and NONHSAT190964.1. In conclusion, the present study identified seven lncRNAs that may serve as potential biological markers for asthma.
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spelling pubmed-89024652022-03-09 Screening and Verification of Differentially Expressed Long Non-coding RNAs in the Peripheral Blood of Patients With asthma Ma, Cheng Wang, Shiyuan Cao, Yuxue Tang, Weifeng Wuniqiemu, Tulake Teng, Fangzhou Zhu, Xueyi Wei, Ying Dong, Jingcheng Front Pharmacol Pharmacology Growing evidence suggests that long non-coding RNAs (lncRNAs) play a key role in the pathogenesis of asthma. Although some differentially expressed lncRNAs have been identified in asthmatic patients, many asthma-related lncRNAs have not been annotated. In the present study, six patients and three healthy subjects were randomly selected from 34 asthmatic patients and 17 healthy subjects. Second-generation high-throughput sequencing was performed on their peripheral blood samples. There were 1,137 differentially expressed lncRNAs in the asthma patients compared to in the healthy controls, of which 485 were upregulated and 652 were downregulated. The top 30 enriched GO and KEGG terms were identified, and the cytosolic ribosome (GO:0022626) and ribosome (hsa03010) were associated with the most differentially expressed lncRNAs. The top 10 differentially expressed lncRNAs associated with asthma were verified by an lncRNA-mRNA co-expression network and RT-qPCR. Seven of the these (NONHSAT015495.2, MSTRG.71212.2, NONHSAT163272.1, NONHSAT181891.1, NONHSAT190964.1, ENST00000564809, and NONHSAT076890.2) were down-regulated in the peripheral blood of asthmatic patients, which was consistent with the sequencing results. Three patients and three healthy subjects were randomly selected from the remaining subjects to verify these seven lncRNAs by RT-qPCR, which further confirmed the sequencing results. Public database GSE106230 was also in agreement with the FPKM (Fragments Per kilobase of exon model per Million mapped reads) trends of ENST00000564809, NONHSAT015495.2, NONHSAT181891.1, and NONHSAT190964.1. In conclusion, the present study identified seven lncRNAs that may serve as potential biological markers for asthma. Frontiers Media S.A. 2022-02-22 /pmc/articles/PMC8902465/ /pubmed/35273507 http://dx.doi.org/10.3389/fphar.2022.834009 Text en Copyright © 2022 Ma, Wang, Cao, Tang, Wuniqiemu, Teng, Zhu, Wei and Dong. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Ma, Cheng
Wang, Shiyuan
Cao, Yuxue
Tang, Weifeng
Wuniqiemu, Tulake
Teng, Fangzhou
Zhu, Xueyi
Wei, Ying
Dong, Jingcheng
Screening and Verification of Differentially Expressed Long Non-coding RNAs in the Peripheral Blood of Patients With asthma
title Screening and Verification of Differentially Expressed Long Non-coding RNAs in the Peripheral Blood of Patients With asthma
title_full Screening and Verification of Differentially Expressed Long Non-coding RNAs in the Peripheral Blood of Patients With asthma
title_fullStr Screening and Verification of Differentially Expressed Long Non-coding RNAs in the Peripheral Blood of Patients With asthma
title_full_unstemmed Screening and Verification of Differentially Expressed Long Non-coding RNAs in the Peripheral Blood of Patients With asthma
title_short Screening and Verification of Differentially Expressed Long Non-coding RNAs in the Peripheral Blood of Patients With asthma
title_sort screening and verification of differentially expressed long non-coding rnas in the peripheral blood of patients with asthma
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8902465/
https://www.ncbi.nlm.nih.gov/pubmed/35273507
http://dx.doi.org/10.3389/fphar.2022.834009
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