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Application Progress of Organoids in Colorectal Cancer

Currently, colorectal cancer is still the third leading cause of cancer-related mortality, and the incidence is rising. It is a long time since the researchers used cancer cell lines and animals as the study subject. However, these models possess various limitations to reflect the cancer progression...

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Autores principales: Luo, Lianxiang, Ma, Yucui, Zheng, Yilin, Su, Jiating, Huang, Guoxin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8902504/
https://www.ncbi.nlm.nih.gov/pubmed/35273961
http://dx.doi.org/10.3389/fcell.2022.815067
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author Luo, Lianxiang
Ma, Yucui
Zheng, Yilin
Su, Jiating
Huang, Guoxin
author_facet Luo, Lianxiang
Ma, Yucui
Zheng, Yilin
Su, Jiating
Huang, Guoxin
author_sort Luo, Lianxiang
collection PubMed
description Currently, colorectal cancer is still the third leading cause of cancer-related mortality, and the incidence is rising. It is a long time since the researchers used cancer cell lines and animals as the study subject. However, these models possess various limitations to reflect the cancer progression in the human body. Organoids have more clinical significance than cell lines, and they also bridge the gap between animal models and humans. Patient-derived organoids are three-dimensional cultures that simulate the tumor characteristics in vivo and recapitulate tumor cell heterogeneity. Therefore, the emergence of colorectal cancer organoids provides an unprecedented opportunity for colorectal cancer research. It retains the molecular and cellular composition of the original tumor and has a high degree of homology and complexity with patient tissues. Patient-derived colorectal cancer organoids, as personalized tumor organoids, can more accurately simulate colorectal cancer patients’ occurrence, development, metastasis, and predict drug response in colorectal cancer patients. Colorectal cancer organoids show great potential for application, especially preclinical drug screening and prediction of patient response to selected treatment options. Here, we reviewed the application of colorectal cancer organoids in disease model construction, basic biological research, organoid biobank construction, drug screening and personalized medicine, drug development, drug toxicity and safety, and regenerative medicine. In addition, we also displayed the current limitations and challenges of organoids and discussed the future development direction of organoids in combination with other technologies. Finally, we summarized and analyzed the current clinical trial research of organoids, especially the clinical trials of colorectal cancer organoids. We hoped to lay a solid foundation for organoids used in colorectal cancer research.
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spelling pubmed-89025042022-03-09 Application Progress of Organoids in Colorectal Cancer Luo, Lianxiang Ma, Yucui Zheng, Yilin Su, Jiating Huang, Guoxin Front Cell Dev Biol Cell and Developmental Biology Currently, colorectal cancer is still the third leading cause of cancer-related mortality, and the incidence is rising. It is a long time since the researchers used cancer cell lines and animals as the study subject. However, these models possess various limitations to reflect the cancer progression in the human body. Organoids have more clinical significance than cell lines, and they also bridge the gap between animal models and humans. Patient-derived organoids are three-dimensional cultures that simulate the tumor characteristics in vivo and recapitulate tumor cell heterogeneity. Therefore, the emergence of colorectal cancer organoids provides an unprecedented opportunity for colorectal cancer research. It retains the molecular and cellular composition of the original tumor and has a high degree of homology and complexity with patient tissues. Patient-derived colorectal cancer organoids, as personalized tumor organoids, can more accurately simulate colorectal cancer patients’ occurrence, development, metastasis, and predict drug response in colorectal cancer patients. Colorectal cancer organoids show great potential for application, especially preclinical drug screening and prediction of patient response to selected treatment options. Here, we reviewed the application of colorectal cancer organoids in disease model construction, basic biological research, organoid biobank construction, drug screening and personalized medicine, drug development, drug toxicity and safety, and regenerative medicine. In addition, we also displayed the current limitations and challenges of organoids and discussed the future development direction of organoids in combination with other technologies. Finally, we summarized and analyzed the current clinical trial research of organoids, especially the clinical trials of colorectal cancer organoids. We hoped to lay a solid foundation for organoids used in colorectal cancer research. Frontiers Media S.A. 2022-02-22 /pmc/articles/PMC8902504/ /pubmed/35273961 http://dx.doi.org/10.3389/fcell.2022.815067 Text en Copyright © 2022 Luo, Ma, Zheng, Su and Huang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Luo, Lianxiang
Ma, Yucui
Zheng, Yilin
Su, Jiating
Huang, Guoxin
Application Progress of Organoids in Colorectal Cancer
title Application Progress of Organoids in Colorectal Cancer
title_full Application Progress of Organoids in Colorectal Cancer
title_fullStr Application Progress of Organoids in Colorectal Cancer
title_full_unstemmed Application Progress of Organoids in Colorectal Cancer
title_short Application Progress of Organoids in Colorectal Cancer
title_sort application progress of organoids in colorectal cancer
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8902504/
https://www.ncbi.nlm.nih.gov/pubmed/35273961
http://dx.doi.org/10.3389/fcell.2022.815067
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