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An interferon-related signature characterizes the whole blood transcriptome profile of insulin-resistant individuals—the CODAM study

BACKGROUND: Worldwide, the prevalence of obesity and insulin resistance has grown dramatically. Gene expression profiling in blood represents a powerful means to explore disease pathogenesis, but the potential impact of inter-individual differences in a cell-type profile is not always taken into acc...

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Autores principales: Kalafati, Marianthi, Kutmon, Martina, Evelo, Chris T., van der Kallen, Carla J. H., Schalkwijk, Casper G., Stehouwer, Coen D. A., Consortium, B. I. O. S., Blaak, Ellen E., van Greevenbroek, Marleen M. J., Adriaens, Michiel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8903498/
https://www.ncbi.nlm.nih.gov/pubmed/34886800
http://dx.doi.org/10.1186/s12263-021-00702-7
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author Kalafati, Marianthi
Kutmon, Martina
Evelo, Chris T.
van der Kallen, Carla J. H.
Schalkwijk, Casper G.
Stehouwer, Coen D. A.
Consortium, B. I. O. S.
Blaak, Ellen E.
van Greevenbroek, Marleen M. J.
Adriaens, Michiel
author_facet Kalafati, Marianthi
Kutmon, Martina
Evelo, Chris T.
van der Kallen, Carla J. H.
Schalkwijk, Casper G.
Stehouwer, Coen D. A.
Consortium, B. I. O. S.
Blaak, Ellen E.
van Greevenbroek, Marleen M. J.
Adriaens, Michiel
author_sort Kalafati, Marianthi
collection PubMed
description BACKGROUND: Worldwide, the prevalence of obesity and insulin resistance has grown dramatically. Gene expression profiling in blood represents a powerful means to explore disease pathogenesis, but the potential impact of inter-individual differences in a cell-type profile is not always taken into account. The objective of this project was to investigate the whole blood transcriptome profile of insulin-resistant as compared to insulin-sensitive individuals independent of inter-individual differences in white blood cell profile. RESULTS: We report a 3% higher relative amount of monocytes in the insulin-resistant individuals. Furthermore, independent of their white blood cell profile, insulin-resistant participants had (i) higher expression of interferon-stimulated genes and (ii) lower expression of genes involved in cellular differentiation and remodeling of the actin cytoskeleton. CONCLUSIONS: We present an approach to investigate the whole blood transcriptome of insulin-resistant individuals, independent of their DNA methylation-derived white blood cell profile. An interferon-related signature characterizes the whole blood transcriptome profile of the insulin-resistant individuals, independent of their white blood cell profile. The observed signature indicates increased systemic inflammation possibly due to an innate immune response and whole-body insulin resistance, which can be a cause or a consequence of insulin resistance. Altered gene expression in specific organs may be reflected in whole blood; hence, our results may reflect obesity and/or insulin resistance-related organ dysfunction in the insulin-resistant individuals. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12263-021-00702-7.
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spelling pubmed-89034982022-03-23 An interferon-related signature characterizes the whole blood transcriptome profile of insulin-resistant individuals—the CODAM study Kalafati, Marianthi Kutmon, Martina Evelo, Chris T. van der Kallen, Carla J. H. Schalkwijk, Casper G. Stehouwer, Coen D. A. Consortium, B. I. O. S. Blaak, Ellen E. van Greevenbroek, Marleen M. J. Adriaens, Michiel Genes Nutr Research BACKGROUND: Worldwide, the prevalence of obesity and insulin resistance has grown dramatically. Gene expression profiling in blood represents a powerful means to explore disease pathogenesis, but the potential impact of inter-individual differences in a cell-type profile is not always taken into account. The objective of this project was to investigate the whole blood transcriptome profile of insulin-resistant as compared to insulin-sensitive individuals independent of inter-individual differences in white blood cell profile. RESULTS: We report a 3% higher relative amount of monocytes in the insulin-resistant individuals. Furthermore, independent of their white blood cell profile, insulin-resistant participants had (i) higher expression of interferon-stimulated genes and (ii) lower expression of genes involved in cellular differentiation and remodeling of the actin cytoskeleton. CONCLUSIONS: We present an approach to investigate the whole blood transcriptome of insulin-resistant individuals, independent of their DNA methylation-derived white blood cell profile. An interferon-related signature characterizes the whole blood transcriptome profile of the insulin-resistant individuals, independent of their white blood cell profile. The observed signature indicates increased systemic inflammation possibly due to an innate immune response and whole-body insulin resistance, which can be a cause or a consequence of insulin resistance. Altered gene expression in specific organs may be reflected in whole blood; hence, our results may reflect obesity and/or insulin resistance-related organ dysfunction in the insulin-resistant individuals. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12263-021-00702-7. BioMed Central 2021-12-09 /pmc/articles/PMC8903498/ /pubmed/34886800 http://dx.doi.org/10.1186/s12263-021-00702-7 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research
Kalafati, Marianthi
Kutmon, Martina
Evelo, Chris T.
van der Kallen, Carla J. H.
Schalkwijk, Casper G.
Stehouwer, Coen D. A.
Consortium, B. I. O. S.
Blaak, Ellen E.
van Greevenbroek, Marleen M. J.
Adriaens, Michiel
An interferon-related signature characterizes the whole blood transcriptome profile of insulin-resistant individuals—the CODAM study
title An interferon-related signature characterizes the whole blood transcriptome profile of insulin-resistant individuals—the CODAM study
title_full An interferon-related signature characterizes the whole blood transcriptome profile of insulin-resistant individuals—the CODAM study
title_fullStr An interferon-related signature characterizes the whole blood transcriptome profile of insulin-resistant individuals—the CODAM study
title_full_unstemmed An interferon-related signature characterizes the whole blood transcriptome profile of insulin-resistant individuals—the CODAM study
title_short An interferon-related signature characterizes the whole blood transcriptome profile of insulin-resistant individuals—the CODAM study
title_sort interferon-related signature characterizes the whole blood transcriptome profile of insulin-resistant individuals—the codam study
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8903498/
https://www.ncbi.nlm.nih.gov/pubmed/34886800
http://dx.doi.org/10.1186/s12263-021-00702-7
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