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Development of a CHO cell line for stable production of recombinant antibodies against human MMP9
BACKGROUND: Human matrix metalloproteinase 9 (hMMP9) is a biomarker in several diseases, including cancer, and the need for developing detectors and inhibitors of hMMP9 is increasing. As an antibody against hMMP9 can be selectively bound to hMMP9, the use of anti-MMP9 antibody presents new possibili...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8903741/ https://www.ncbi.nlm.nih.gov/pubmed/35255869 http://dx.doi.org/10.1186/s12896-022-00738-6 |
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author | Ryu, Jina Kim, Eun-Jung Kim, Joo-Kyung Park, Tai Hyun Kim, Byung-Gee Jeong, Hee-Jin |
author_facet | Ryu, Jina Kim, Eun-Jung Kim, Joo-Kyung Park, Tai Hyun Kim, Byung-Gee Jeong, Hee-Jin |
author_sort | Ryu, Jina |
collection | PubMed |
description | BACKGROUND: Human matrix metalloproteinase 9 (hMMP9) is a biomarker in several diseases, including cancer, and the need for developing detectors and inhibitors of hMMP9 is increasing. As an antibody against hMMP9 can be selectively bound to hMMP9, the use of anti-MMP9 antibody presents new possibilities to address hMMP9-related diseases. In this study, we aimed to establish a stable Chinese hamster ovary (CHO) cell line for the stable production of antibodies against hMMP9. RESULTS: Weconstructed recombinant anti-hMMP9 antibody fragment-expressing genes and transfected these to CHO cells. We chose a single clone, and successfully produced a full-sized antibody against hMMP9 with high purity, sensitivity, and reproducibility. Subsequently, we confirmed the antigen-binding efficiency of the antibody. CONCLUSIONS: We developed a novel recombinant anti-hMMP9 antibody via a CHO cell-based mammalian expression system, which has a high potential to be used in a broad range of medical and industrial areas. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12896-022-00738-6. |
format | Online Article Text |
id | pubmed-8903741 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-89037412022-03-18 Development of a CHO cell line for stable production of recombinant antibodies against human MMP9 Ryu, Jina Kim, Eun-Jung Kim, Joo-Kyung Park, Tai Hyun Kim, Byung-Gee Jeong, Hee-Jin BMC Biotechnol Research BACKGROUND: Human matrix metalloproteinase 9 (hMMP9) is a biomarker in several diseases, including cancer, and the need for developing detectors and inhibitors of hMMP9 is increasing. As an antibody against hMMP9 can be selectively bound to hMMP9, the use of anti-MMP9 antibody presents new possibilities to address hMMP9-related diseases. In this study, we aimed to establish a stable Chinese hamster ovary (CHO) cell line for the stable production of antibodies against hMMP9. RESULTS: Weconstructed recombinant anti-hMMP9 antibody fragment-expressing genes and transfected these to CHO cells. We chose a single clone, and successfully produced a full-sized antibody against hMMP9 with high purity, sensitivity, and reproducibility. Subsequently, we confirmed the antigen-binding efficiency of the antibody. CONCLUSIONS: We developed a novel recombinant anti-hMMP9 antibody via a CHO cell-based mammalian expression system, which has a high potential to be used in a broad range of medical and industrial areas. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12896-022-00738-6. BioMed Central 2022-03-07 /pmc/articles/PMC8903741/ /pubmed/35255869 http://dx.doi.org/10.1186/s12896-022-00738-6 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Ryu, Jina Kim, Eun-Jung Kim, Joo-Kyung Park, Tai Hyun Kim, Byung-Gee Jeong, Hee-Jin Development of a CHO cell line for stable production of recombinant antibodies against human MMP9 |
title | Development of a CHO cell line for stable production of recombinant antibodies against human MMP9 |
title_full | Development of a CHO cell line for stable production of recombinant antibodies against human MMP9 |
title_fullStr | Development of a CHO cell line for stable production of recombinant antibodies against human MMP9 |
title_full_unstemmed | Development of a CHO cell line for stable production of recombinant antibodies against human MMP9 |
title_short | Development of a CHO cell line for stable production of recombinant antibodies against human MMP9 |
title_sort | development of a cho cell line for stable production of recombinant antibodies against human mmp9 |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8903741/ https://www.ncbi.nlm.nih.gov/pubmed/35255869 http://dx.doi.org/10.1186/s12896-022-00738-6 |
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