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Antifatigue Effect of Panax Notoginseng Leaves Fermented With Microorganisms: In-vitro and In-vivo Evaluation

Fatigue is a common physiological phenomenon caused by many complicated factors. Excessive fatigue will lead to a series of uncomfortable reactions and damage body health. Panax notoginseng leaves (PNL) is a new resource food that good for soothing nerves, nourishing the heart, and strengthening the...

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Autores principales: Yang, Min, Tao, Liang, Zhao, Cun-Chao, Wang, Zi-Lin, Yu, Zhi-Jin, Zhou, Wen, Wen, Yan-Long, Li, Ling-Fei, Tian, Yang, Sheng, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8904179/
https://www.ncbi.nlm.nih.gov/pubmed/35273989
http://dx.doi.org/10.3389/fnut.2022.824525
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author Yang, Min
Tao, Liang
Zhao, Cun-Chao
Wang, Zi-Lin
Yu, Zhi-Jin
Zhou, Wen
Wen, Yan-Long
Li, Ling-Fei
Tian, Yang
Sheng, Jun
author_facet Yang, Min
Tao, Liang
Zhao, Cun-Chao
Wang, Zi-Lin
Yu, Zhi-Jin
Zhou, Wen
Wen, Yan-Long
Li, Ling-Fei
Tian, Yang
Sheng, Jun
author_sort Yang, Min
collection PubMed
description Fatigue is a common physiological phenomenon caused by many complicated factors. Excessive fatigue will lead to a series of uncomfortable reactions and damage body health. Panax notoginseng leaves (PNL) is a new resource food that good for soothing nerves, nourishing the heart, and strengthening the spleen. Microbial fermentation could increase the content of bio-ingredients and produce new active ingredients. However, the effect of fermented P. notoginseng leaves (FPNL) on antifatigue and the molecular mechanisms remain to be elucidated. Thus, in this study, we evaluated the antifatigue effect of co-fermented P. notoginseng leaves by Saccharomyces cerevisiae and Bacillus subtilis in-vitro and in-vivo, and its mechanism was further elucidated. The results showed that FPNL exhibited higher saponins, organic phenolic acids content, and antioxidant activity than PNL. FPNL improved ISO-induced H9c2 myocardial cell damage by alleviating apoptosis (modulating Bax and Bcl-2 protein expression) and reducing antioxidant activity in-vitro. Moreover, in-vivo experiment showed that FPNL significantly prolonged the weight-loading swimming time of mice. After gavaged FPNL, the levels of liver glycogen (LG) and serum lactate dehydrogenase (LDH) activity were increased in mice. In contrast, the levels of blood urea nitrogen (BUN), lactate acid, and malondialdehyde (MDA) were decreased. In summary, our results indicated that FPNL showed a good antifatigue effect in-vivo and in-vitro.
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spelling pubmed-89041792022-03-09 Antifatigue Effect of Panax Notoginseng Leaves Fermented With Microorganisms: In-vitro and In-vivo Evaluation Yang, Min Tao, Liang Zhao, Cun-Chao Wang, Zi-Lin Yu, Zhi-Jin Zhou, Wen Wen, Yan-Long Li, Ling-Fei Tian, Yang Sheng, Jun Front Nutr Nutrition Fatigue is a common physiological phenomenon caused by many complicated factors. Excessive fatigue will lead to a series of uncomfortable reactions and damage body health. Panax notoginseng leaves (PNL) is a new resource food that good for soothing nerves, nourishing the heart, and strengthening the spleen. Microbial fermentation could increase the content of bio-ingredients and produce new active ingredients. However, the effect of fermented P. notoginseng leaves (FPNL) on antifatigue and the molecular mechanisms remain to be elucidated. Thus, in this study, we evaluated the antifatigue effect of co-fermented P. notoginseng leaves by Saccharomyces cerevisiae and Bacillus subtilis in-vitro and in-vivo, and its mechanism was further elucidated. The results showed that FPNL exhibited higher saponins, organic phenolic acids content, and antioxidant activity than PNL. FPNL improved ISO-induced H9c2 myocardial cell damage by alleviating apoptosis (modulating Bax and Bcl-2 protein expression) and reducing antioxidant activity in-vitro. Moreover, in-vivo experiment showed that FPNL significantly prolonged the weight-loading swimming time of mice. After gavaged FPNL, the levels of liver glycogen (LG) and serum lactate dehydrogenase (LDH) activity were increased in mice. In contrast, the levels of blood urea nitrogen (BUN), lactate acid, and malondialdehyde (MDA) were decreased. In summary, our results indicated that FPNL showed a good antifatigue effect in-vivo and in-vitro. Frontiers Media S.A. 2022-02-22 /pmc/articles/PMC8904179/ /pubmed/35273989 http://dx.doi.org/10.3389/fnut.2022.824525 Text en Copyright © 2022 Yang, Tao, Zhao, Wang, Yu, Zhou, Wen, Li, Tian and Sheng. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Nutrition
Yang, Min
Tao, Liang
Zhao, Cun-Chao
Wang, Zi-Lin
Yu, Zhi-Jin
Zhou, Wen
Wen, Yan-Long
Li, Ling-Fei
Tian, Yang
Sheng, Jun
Antifatigue Effect of Panax Notoginseng Leaves Fermented With Microorganisms: In-vitro and In-vivo Evaluation
title Antifatigue Effect of Panax Notoginseng Leaves Fermented With Microorganisms: In-vitro and In-vivo Evaluation
title_full Antifatigue Effect of Panax Notoginseng Leaves Fermented With Microorganisms: In-vitro and In-vivo Evaluation
title_fullStr Antifatigue Effect of Panax Notoginseng Leaves Fermented With Microorganisms: In-vitro and In-vivo Evaluation
title_full_unstemmed Antifatigue Effect of Panax Notoginseng Leaves Fermented With Microorganisms: In-vitro and In-vivo Evaluation
title_short Antifatigue Effect of Panax Notoginseng Leaves Fermented With Microorganisms: In-vitro and In-vivo Evaluation
title_sort antifatigue effect of panax notoginseng leaves fermented with microorganisms: in-vitro and in-vivo evaluation
topic Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8904179/
https://www.ncbi.nlm.nih.gov/pubmed/35273989
http://dx.doi.org/10.3389/fnut.2022.824525
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