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Research Note: Molecular characterization of antimicrobial resistance and virulence gene analysis of Enterococcus faecalis in poultry in Tai'an, China

Enterococcus faecalis (E. faecalis) is a zoonotic pathogen that causes severe economic losses in the poultry-breeding industry. In our study, cecal samples from broilers with cecal enlargement at slaughterhouses in Tai'an, China, were analyzed. The results revealed that the 61 E. faecalis strai...

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Autores principales: Yu, Lanping, Liu, Yu, Liu, Mengda, Li, Zixuan, Li, Lin, Wang, Fangkun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8904221/
https://www.ncbi.nlm.nih.gov/pubmed/35263706
http://dx.doi.org/10.1016/j.psj.2022.101763
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author Yu, Lanping
Liu, Yu
Liu, Mengda
Li, Zixuan
Li, Lin
Wang, Fangkun
author_facet Yu, Lanping
Liu, Yu
Liu, Mengda
Li, Zixuan
Li, Lin
Wang, Fangkun
author_sort Yu, Lanping
collection PubMed
description Enterococcus faecalis (E. faecalis) is a zoonotic pathogen that causes severe economic losses in the poultry-breeding industry. In our study, cecal samples from broilers with cecal enlargement at slaughterhouses in Tai'an, China, were analyzed. The results revealed that the 61 E. faecalis strains had drug resistance rates ranging from 96.72 to 8.20% against 11 antibiotics in 5 classes, of which erythromycin (96.72%) and tetracycline (96.72%) had the highest rates and vancomycin (8.20%) the lowest. The highest detection rate of multiple drug-resistant strains in 61 isolates was 72.13%. The results of polymerase chain reaction showed that, of the 12 virulence genes, ccf had the highest detection rate (80.33%), followed by asal and cob (both 78.69%), whereas hyl had the lowest (6.56%). Among 15 drug resistance genes, ermB had the highest detection rate (95.08%), followed by tetM (91.80%) and tetL (90.16%), whereas tetK (0.00%) and vanB (0.00%) remained undetected. Of the 34 sequence types found with multilocus sequence typing, the most predominant were ST631 (13.11%, 8/61) and ST634 (8.2%, 5/61). Our results provide a theoretical basis for guiding the rational use of antibiotics and preventing the spread of drug-resistant bacteria, along with epidemiological data for the risk analysis of food-borne bacteria and antimicrobial resistance in poultry farms in Shandong Province.
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spelling pubmed-89042212022-03-10 Research Note: Molecular characterization of antimicrobial resistance and virulence gene analysis of Enterococcus faecalis in poultry in Tai'an, China Yu, Lanping Liu, Yu Liu, Mengda Li, Zixuan Li, Lin Wang, Fangkun Poult Sci MICROBIOLOGY AND FOOD SAFETY Enterococcus faecalis (E. faecalis) is a zoonotic pathogen that causes severe economic losses in the poultry-breeding industry. In our study, cecal samples from broilers with cecal enlargement at slaughterhouses in Tai'an, China, were analyzed. The results revealed that the 61 E. faecalis strains had drug resistance rates ranging from 96.72 to 8.20% against 11 antibiotics in 5 classes, of which erythromycin (96.72%) and tetracycline (96.72%) had the highest rates and vancomycin (8.20%) the lowest. The highest detection rate of multiple drug-resistant strains in 61 isolates was 72.13%. The results of polymerase chain reaction showed that, of the 12 virulence genes, ccf had the highest detection rate (80.33%), followed by asal and cob (both 78.69%), whereas hyl had the lowest (6.56%). Among 15 drug resistance genes, ermB had the highest detection rate (95.08%), followed by tetM (91.80%) and tetL (90.16%), whereas tetK (0.00%) and vanB (0.00%) remained undetected. Of the 34 sequence types found with multilocus sequence typing, the most predominant were ST631 (13.11%, 8/61) and ST634 (8.2%, 5/61). Our results provide a theoretical basis for guiding the rational use of antibiotics and preventing the spread of drug-resistant bacteria, along with epidemiological data for the risk analysis of food-borne bacteria and antimicrobial resistance in poultry farms in Shandong Province. Elsevier 2022-01-30 /pmc/articles/PMC8904221/ /pubmed/35263706 http://dx.doi.org/10.1016/j.psj.2022.101763 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle MICROBIOLOGY AND FOOD SAFETY
Yu, Lanping
Liu, Yu
Liu, Mengda
Li, Zixuan
Li, Lin
Wang, Fangkun
Research Note: Molecular characterization of antimicrobial resistance and virulence gene analysis of Enterococcus faecalis in poultry in Tai'an, China
title Research Note: Molecular characterization of antimicrobial resistance and virulence gene analysis of Enterococcus faecalis in poultry in Tai'an, China
title_full Research Note: Molecular characterization of antimicrobial resistance and virulence gene analysis of Enterococcus faecalis in poultry in Tai'an, China
title_fullStr Research Note: Molecular characterization of antimicrobial resistance and virulence gene analysis of Enterococcus faecalis in poultry in Tai'an, China
title_full_unstemmed Research Note: Molecular characterization of antimicrobial resistance and virulence gene analysis of Enterococcus faecalis in poultry in Tai'an, China
title_short Research Note: Molecular characterization of antimicrobial resistance and virulence gene analysis of Enterococcus faecalis in poultry in Tai'an, China
title_sort research note: molecular characterization of antimicrobial resistance and virulence gene analysis of enterococcus faecalis in poultry in tai'an, china
topic MICROBIOLOGY AND FOOD SAFETY
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8904221/
https://www.ncbi.nlm.nih.gov/pubmed/35263706
http://dx.doi.org/10.1016/j.psj.2022.101763
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