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Using Home-Cage Monitoring to Determine the Impact of Timed Mating on Male Mouse Welfare

Some of our breeding programs include the use of Prm1 male Homozygous mice which are naturally sterile. This removes the need to use vasectomized males to induce pseudopregnancy in female mice. These males can be kept for up to 9 months and are housed with a companion female. During the timed mating...

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Autores principales: Moore, Joanna L., Brook, Eloisa I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8904386/
https://www.ncbi.nlm.nih.gov/pubmed/35281486
http://dx.doi.org/10.3389/fnins.2022.786652
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author Moore, Joanna L.
Brook, Eloisa I.
author_facet Moore, Joanna L.
Brook, Eloisa I.
author_sort Moore, Joanna L.
collection PubMed
description Some of our breeding programs include the use of Prm1 male Homozygous mice which are naturally sterile. This removes the need to use vasectomized males to induce pseudopregnancy in female mice. These males can be kept for up to 9 months and are housed with a companion female. During the timed mating period the companion female is replaced with a new female. This procedure can occur at regular intervals causing a significant increase in cage activity; one of our objectives was to determine whether this was as a result of timed mating. We wanted to investigate the disruption caused to mice during the day of the swap and how long it would take for the cage activity to return to pre-replacement baseline levels. We hypothesized that this impact would be reflected as a significant increase in cage activity, which in itself may not be a result of a negative experience but the potential of repeated disruption to their activity pattern should be considered. We used a well-known home-cage monitoring system to assess changes to the activity pattern in cages when a companion female is replaced. Data from our initial study showed that in the 2-h period after the female is replaced there is a significant increase in cage activity compared to the same time frame on the previous day. In the subsequent study, where no cage change occurred, an increase in activity was also observed when females were replaced; this returned to baseline after approximately 4 h. Prolonged activity during the rest period of mice (over 2 h) could lead to them being fatigued during their active period; therefore, as a refinement we propose that timed matings be performed later in the day, at a time when the animals are active.
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spelling pubmed-89043862022-03-10 Using Home-Cage Monitoring to Determine the Impact of Timed Mating on Male Mouse Welfare Moore, Joanna L. Brook, Eloisa I. Front Neurosci Neuroscience Some of our breeding programs include the use of Prm1 male Homozygous mice which are naturally sterile. This removes the need to use vasectomized males to induce pseudopregnancy in female mice. These males can be kept for up to 9 months and are housed with a companion female. During the timed mating period the companion female is replaced with a new female. This procedure can occur at regular intervals causing a significant increase in cage activity; one of our objectives was to determine whether this was as a result of timed mating. We wanted to investigate the disruption caused to mice during the day of the swap and how long it would take for the cage activity to return to pre-replacement baseline levels. We hypothesized that this impact would be reflected as a significant increase in cage activity, which in itself may not be a result of a negative experience but the potential of repeated disruption to their activity pattern should be considered. We used a well-known home-cage monitoring system to assess changes to the activity pattern in cages when a companion female is replaced. Data from our initial study showed that in the 2-h period after the female is replaced there is a significant increase in cage activity compared to the same time frame on the previous day. In the subsequent study, where no cage change occurred, an increase in activity was also observed when females were replaced; this returned to baseline after approximately 4 h. Prolonged activity during the rest period of mice (over 2 h) could lead to them being fatigued during their active period; therefore, as a refinement we propose that timed matings be performed later in the day, at a time when the animals are active. Frontiers Media S.A. 2022-02-23 /pmc/articles/PMC8904386/ /pubmed/35281486 http://dx.doi.org/10.3389/fnins.2022.786652 Text en Copyright © 2022 Moore and Brook. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Moore, Joanna L.
Brook, Eloisa I.
Using Home-Cage Monitoring to Determine the Impact of Timed Mating on Male Mouse Welfare
title Using Home-Cage Monitoring to Determine the Impact of Timed Mating on Male Mouse Welfare
title_full Using Home-Cage Monitoring to Determine the Impact of Timed Mating on Male Mouse Welfare
title_fullStr Using Home-Cage Monitoring to Determine the Impact of Timed Mating on Male Mouse Welfare
title_full_unstemmed Using Home-Cage Monitoring to Determine the Impact of Timed Mating on Male Mouse Welfare
title_short Using Home-Cage Monitoring to Determine the Impact of Timed Mating on Male Mouse Welfare
title_sort using home-cage monitoring to determine the impact of timed mating on male mouse welfare
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8904386/
https://www.ncbi.nlm.nih.gov/pubmed/35281486
http://dx.doi.org/10.3389/fnins.2022.786652
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