A NanoBiT assay to monitor membrane proteins trafficking for drug discovery and drug development

Internalization of membrane proteins plays a key role in many physiological functions; however, highly sensitive and versatile technologies are lacking to study such processes in real-time living systems. Here we describe an assay based on bioluminescence able to quantify membrane receptor trafficki...

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Detalles Bibliográficos
Autores principales: Reyes-Alcaraz, Arfaxad, Lucero Garcia-Rojas, Emilio Y., Merlinsky, Elizabeth A., Seong, Jae Young, Bond, Richard A., McConnell, Bradley K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8904512/
https://www.ncbi.nlm.nih.gov/pubmed/35260793
http://dx.doi.org/10.1038/s42003-022-03163-9
Descripción
Sumario:Internalization of membrane proteins plays a key role in many physiological functions; however, highly sensitive and versatile technologies are lacking to study such processes in real-time living systems. Here we describe an assay based on bioluminescence able to quantify membrane receptor trafficking for a wide variety of internalization mechanisms such as GPCR internalization/recycling, antibody-mediated internalization, and SARS-CoV2 viral infection. This study represents an alternative drug discovery tool to accelerate the drug development for a wide range of physiological processes, such as cancer, neurological, cardiopulmonary, metabolic, and infectious diseases including COVID-19.

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