A NanoBiT assay to monitor membrane proteins trafficking for drug discovery and drug development

Internalization of membrane proteins plays a key role in many physiological functions; however, highly sensitive and versatile technologies are lacking to study such processes in real-time living systems. Here we describe an assay based on bioluminescence able to quantify membrane receptor trafficki...

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Autores principales: Reyes-Alcaraz, Arfaxad, Lucero Garcia-Rojas, Emilio Y., Merlinsky, Elizabeth A., Seong, Jae Young, Bond, Richard A., McConnell, Bradley K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8904512/
https://www.ncbi.nlm.nih.gov/pubmed/35260793
http://dx.doi.org/10.1038/s42003-022-03163-9
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author Reyes-Alcaraz, Arfaxad
Lucero Garcia-Rojas, Emilio Y.
Merlinsky, Elizabeth A.
Seong, Jae Young
Bond, Richard A.
McConnell, Bradley K.
author_facet Reyes-Alcaraz, Arfaxad
Lucero Garcia-Rojas, Emilio Y.
Merlinsky, Elizabeth A.
Seong, Jae Young
Bond, Richard A.
McConnell, Bradley K.
author_sort Reyes-Alcaraz, Arfaxad
collection PubMed
description Internalization of membrane proteins plays a key role in many physiological functions; however, highly sensitive and versatile technologies are lacking to study such processes in real-time living systems. Here we describe an assay based on bioluminescence able to quantify membrane receptor trafficking for a wide variety of internalization mechanisms such as GPCR internalization/recycling, antibody-mediated internalization, and SARS-CoV2 viral infection. This study represents an alternative drug discovery tool to accelerate the drug development for a wide range of physiological processes, such as cancer, neurological, cardiopulmonary, metabolic, and infectious diseases including COVID-19.
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spelling pubmed-89045122022-03-23 A NanoBiT assay to monitor membrane proteins trafficking for drug discovery and drug development Reyes-Alcaraz, Arfaxad Lucero Garcia-Rojas, Emilio Y. Merlinsky, Elizabeth A. Seong, Jae Young Bond, Richard A. McConnell, Bradley K. Commun Biol Article Internalization of membrane proteins plays a key role in many physiological functions; however, highly sensitive and versatile technologies are lacking to study such processes in real-time living systems. Here we describe an assay based on bioluminescence able to quantify membrane receptor trafficking for a wide variety of internalization mechanisms such as GPCR internalization/recycling, antibody-mediated internalization, and SARS-CoV2 viral infection. This study represents an alternative drug discovery tool to accelerate the drug development for a wide range of physiological processes, such as cancer, neurological, cardiopulmonary, metabolic, and infectious diseases including COVID-19. Nature Publishing Group UK 2022-03-08 /pmc/articles/PMC8904512/ /pubmed/35260793 http://dx.doi.org/10.1038/s42003-022-03163-9 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Reyes-Alcaraz, Arfaxad
Lucero Garcia-Rojas, Emilio Y.
Merlinsky, Elizabeth A.
Seong, Jae Young
Bond, Richard A.
McConnell, Bradley K.
A NanoBiT assay to monitor membrane proteins trafficking for drug discovery and drug development
title A NanoBiT assay to monitor membrane proteins trafficking for drug discovery and drug development
title_full A NanoBiT assay to monitor membrane proteins trafficking for drug discovery and drug development
title_fullStr A NanoBiT assay to monitor membrane proteins trafficking for drug discovery and drug development
title_full_unstemmed A NanoBiT assay to monitor membrane proteins trafficking for drug discovery and drug development
title_short A NanoBiT assay to monitor membrane proteins trafficking for drug discovery and drug development
title_sort nanobit assay to monitor membrane proteins trafficking for drug discovery and drug development
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8904512/
https://www.ncbi.nlm.nih.gov/pubmed/35260793
http://dx.doi.org/10.1038/s42003-022-03163-9
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