Solvent Front Position Extraction and some conventional sample preparation techniques for the determination of coccidiostats in poultry feed by LC–MS/MS

Solvent Front Position Extraction is a novel technique developed for effective sample preparation of biological samples containing coccidiostats prior to LC–MS/MS. In this study the technique was used for isolation and determination of seven coccidiostats, from both main groups being: ionophores and...

Descripción completa

Detalles Bibliográficos
Autores principales: Rybicki, Maciej Jan, Becue, Ilse, Daeseleire, Els, Klimek-Turek, Anna, Dzido, Tadeusz Henryk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8904545/
https://www.ncbi.nlm.nih.gov/pubmed/35260618
http://dx.doi.org/10.1038/s41598-022-07587-5
_version_ 1784664975741026304
author Rybicki, Maciej Jan
Becue, Ilse
Daeseleire, Els
Klimek-Turek, Anna
Dzido, Tadeusz Henryk
author_facet Rybicki, Maciej Jan
Becue, Ilse
Daeseleire, Els
Klimek-Turek, Anna
Dzido, Tadeusz Henryk
author_sort Rybicki, Maciej Jan
collection PubMed
description Solvent Front Position Extraction is a novel technique developed for effective sample preparation of biological samples containing coccidiostats prior to LC–MS/MS. In this study the technique was used for isolation and determination of seven coccidiostats, from both main groups being: ionophores and chemical coccidiostats. Its effectiveness was evaluated by comparing with other sample preparation procedures, used in European routine laboratories. Results obtained by Solvent Front Position Extraction were very satisfactory (linearity R(2) ≥ 0.971, recovery 90.1–111.1%, RSDr: 8.7–16.6%, RSD(R): 9.0–17.7%) and fulfilled requirements described in Commission Regulation (EU) 2021/808 of 22 March 2021, which showed great potential of the technique in sample preparation of coccidiostats in poultry feed.
format Online
Article
Text
id pubmed-8904545
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-89045452022-03-09 Solvent Front Position Extraction and some conventional sample preparation techniques for the determination of coccidiostats in poultry feed by LC–MS/MS Rybicki, Maciej Jan Becue, Ilse Daeseleire, Els Klimek-Turek, Anna Dzido, Tadeusz Henryk Sci Rep Article Solvent Front Position Extraction is a novel technique developed for effective sample preparation of biological samples containing coccidiostats prior to LC–MS/MS. In this study the technique was used for isolation and determination of seven coccidiostats, from both main groups being: ionophores and chemical coccidiostats. Its effectiveness was evaluated by comparing with other sample preparation procedures, used in European routine laboratories. Results obtained by Solvent Front Position Extraction were very satisfactory (linearity R(2) ≥ 0.971, recovery 90.1–111.1%, RSDr: 8.7–16.6%, RSD(R): 9.0–17.7%) and fulfilled requirements described in Commission Regulation (EU) 2021/808 of 22 March 2021, which showed great potential of the technique in sample preparation of coccidiostats in poultry feed. Nature Publishing Group UK 2022-03-08 /pmc/articles/PMC8904545/ /pubmed/35260618 http://dx.doi.org/10.1038/s41598-022-07587-5 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Rybicki, Maciej Jan
Becue, Ilse
Daeseleire, Els
Klimek-Turek, Anna
Dzido, Tadeusz Henryk
Solvent Front Position Extraction and some conventional sample preparation techniques for the determination of coccidiostats in poultry feed by LC–MS/MS
title Solvent Front Position Extraction and some conventional sample preparation techniques for the determination of coccidiostats in poultry feed by LC–MS/MS
title_full Solvent Front Position Extraction and some conventional sample preparation techniques for the determination of coccidiostats in poultry feed by LC–MS/MS
title_fullStr Solvent Front Position Extraction and some conventional sample preparation techniques for the determination of coccidiostats in poultry feed by LC–MS/MS
title_full_unstemmed Solvent Front Position Extraction and some conventional sample preparation techniques for the determination of coccidiostats in poultry feed by LC–MS/MS
title_short Solvent Front Position Extraction and some conventional sample preparation techniques for the determination of coccidiostats in poultry feed by LC–MS/MS
title_sort solvent front position extraction and some conventional sample preparation techniques for the determination of coccidiostats in poultry feed by lc–ms/ms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8904545/
https://www.ncbi.nlm.nih.gov/pubmed/35260618
http://dx.doi.org/10.1038/s41598-022-07587-5
work_keys_str_mv AT rybickimaciejjan solventfrontpositionextractionandsomeconventionalsamplepreparationtechniquesforthedeterminationofcoccidiostatsinpoultryfeedbylcmsms
AT becueilse solventfrontpositionextractionandsomeconventionalsamplepreparationtechniquesforthedeterminationofcoccidiostatsinpoultryfeedbylcmsms
AT daeseleireels solventfrontpositionextractionandsomeconventionalsamplepreparationtechniquesforthedeterminationofcoccidiostatsinpoultryfeedbylcmsms
AT klimekturekanna solventfrontpositionextractionandsomeconventionalsamplepreparationtechniquesforthedeterminationofcoccidiostatsinpoultryfeedbylcmsms
AT dzidotadeuszhenryk solventfrontpositionextractionandsomeconventionalsamplepreparationtechniquesforthedeterminationofcoccidiostatsinpoultryfeedbylcmsms

Ejemplares similares