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YB1 associates with oncogenetic roles and poor prognosis in nasopharyngeal carcinoma
Nasopharyngeal carcinoma (NPC) is the malignant tumor arising from the nasopharynx epithelium with ethnic and geographical distribution preference. Y-box binding protein-1 (YB1) is the highly expressed DNA/RNA-binding protein with cold shock domain, and enhanced YB1 expression was proved to be assoc...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8904596/ https://www.ncbi.nlm.nih.gov/pubmed/35260638 http://dx.doi.org/10.1038/s41598-022-07636-z |
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author | Zhan, Yuting Chen, Xianyong Zheng, Hongmei Luo, Jiadi Yang, Yang Ning, Yue Wang, Haihua Zhang, Yuting Zhou, Ming Wang, Weiyuan Fan, Songqing |
author_facet | Zhan, Yuting Chen, Xianyong Zheng, Hongmei Luo, Jiadi Yang, Yang Ning, Yue Wang, Haihua Zhang, Yuting Zhou, Ming Wang, Weiyuan Fan, Songqing |
author_sort | Zhan, Yuting |
collection | PubMed |
description | Nasopharyngeal carcinoma (NPC) is the malignant tumor arising from the nasopharynx epithelium with ethnic and geographical distribution preference. Y-box binding protein-1 (YB1) is the highly expressed DNA/RNA-binding protein with cold shock domain, and enhanced YB1 expression was proved to be associated with many kinds of malignant tumors. There is no systematic study about the regulation of YB1 and cell proliferation, migration, invasion and stress granules (SGs) in NPC, and the relationship between YB1 expression and clinical characteristics and prognosis of NPC patients. We analyzed the mRNA expression of YBX1 in head and neck squamous carcinoma (HNSC) and NPC in databases, investigated the functions of YB1 in cell proliferation, migration and invasion and SGs formation of NPC cells, and detected expression of YB1 protein in a large scale of NPC samples and analyzed their association with clinicopathological features and prognostic significance of NPC patients. YBX1 mRNA was significantly high expression in HNSC and NPC by bioinformatic analysis, and higher expression of YBX1 mRNA indicated poorer prognosis of HNSC patients. Clinically, the expression of YB1 in NPC tissues was significantly higher than these in the control nasopharyngeal epithelial tissues. We further found that the expression of YB1 had an evidently positive relation with advanced clinical stages of patients with NPC. The overall survival rates (OS) were significantly lower for NPC patients with positive expression of YB1. Multivariate analysis confirmed that positive expression of YB1 was the independent poorer prognostic factor for patients with NPC. Moreover, compared with the immortalized nasopharyngeal epithelial cell line (NP69), the basal level of YB1 in NPC cell lines was significantly higher. Knocking down YB1 may inhibit Akt/mTOR pathway in NPC cells. Knocking down YB1 by small interfering RNAs can reduce the ability of proliferation, migration, invasion and SGs formation of NPC cells. The expression of YB1 in NPC cell lines or patients with NPC was significantly higher. The high expression of YB1 protein may act as one valuable independent biomarker to predict poor prognosis for patients with NPC. Knocking down YB1 may release the malignant phenotype of NPC cells. |
format | Online Article Text |
id | pubmed-8904596 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-89045962022-03-09 YB1 associates with oncogenetic roles and poor prognosis in nasopharyngeal carcinoma Zhan, Yuting Chen, Xianyong Zheng, Hongmei Luo, Jiadi Yang, Yang Ning, Yue Wang, Haihua Zhang, Yuting Zhou, Ming Wang, Weiyuan Fan, Songqing Sci Rep Article Nasopharyngeal carcinoma (NPC) is the malignant tumor arising from the nasopharynx epithelium with ethnic and geographical distribution preference. Y-box binding protein-1 (YB1) is the highly expressed DNA/RNA-binding protein with cold shock domain, and enhanced YB1 expression was proved to be associated with many kinds of malignant tumors. There is no systematic study about the regulation of YB1 and cell proliferation, migration, invasion and stress granules (SGs) in NPC, and the relationship between YB1 expression and clinical characteristics and prognosis of NPC patients. We analyzed the mRNA expression of YBX1 in head and neck squamous carcinoma (HNSC) and NPC in databases, investigated the functions of YB1 in cell proliferation, migration and invasion and SGs formation of NPC cells, and detected expression of YB1 protein in a large scale of NPC samples and analyzed their association with clinicopathological features and prognostic significance of NPC patients. YBX1 mRNA was significantly high expression in HNSC and NPC by bioinformatic analysis, and higher expression of YBX1 mRNA indicated poorer prognosis of HNSC patients. Clinically, the expression of YB1 in NPC tissues was significantly higher than these in the control nasopharyngeal epithelial tissues. We further found that the expression of YB1 had an evidently positive relation with advanced clinical stages of patients with NPC. The overall survival rates (OS) were significantly lower for NPC patients with positive expression of YB1. Multivariate analysis confirmed that positive expression of YB1 was the independent poorer prognostic factor for patients with NPC. Moreover, compared with the immortalized nasopharyngeal epithelial cell line (NP69), the basal level of YB1 in NPC cell lines was significantly higher. Knocking down YB1 may inhibit Akt/mTOR pathway in NPC cells. Knocking down YB1 by small interfering RNAs can reduce the ability of proliferation, migration, invasion and SGs formation of NPC cells. The expression of YB1 in NPC cell lines or patients with NPC was significantly higher. The high expression of YB1 protein may act as one valuable independent biomarker to predict poor prognosis for patients with NPC. Knocking down YB1 may release the malignant phenotype of NPC cells. Nature Publishing Group UK 2022-03-08 /pmc/articles/PMC8904596/ /pubmed/35260638 http://dx.doi.org/10.1038/s41598-022-07636-z Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Zhan, Yuting Chen, Xianyong Zheng, Hongmei Luo, Jiadi Yang, Yang Ning, Yue Wang, Haihua Zhang, Yuting Zhou, Ming Wang, Weiyuan Fan, Songqing YB1 associates with oncogenetic roles and poor prognosis in nasopharyngeal carcinoma |
title | YB1 associates with oncogenetic roles and poor prognosis in nasopharyngeal carcinoma |
title_full | YB1 associates with oncogenetic roles and poor prognosis in nasopharyngeal carcinoma |
title_fullStr | YB1 associates with oncogenetic roles and poor prognosis in nasopharyngeal carcinoma |
title_full_unstemmed | YB1 associates with oncogenetic roles and poor prognosis in nasopharyngeal carcinoma |
title_short | YB1 associates with oncogenetic roles and poor prognosis in nasopharyngeal carcinoma |
title_sort | yb1 associates with oncogenetic roles and poor prognosis in nasopharyngeal carcinoma |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8904596/ https://www.ncbi.nlm.nih.gov/pubmed/35260638 http://dx.doi.org/10.1038/s41598-022-07636-z |
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