Protocol to assess the impact of early-life antibiotic exposure on murine longevity

We present this protocol using a mouse model to assess the impact of early-life antibiotic exposure on mammalian lifespan and the composition of the gut microbiota over time. We describe longitudinal fecal sampling and health monitoring following early-life antibiotic exposure. We detail DNA extract...

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Detalles Bibliográficos
Autores principales: Lynn, Miriam A., Ryan, Feargal J., Tee, Yee C., Benson, Saoirse C., Lynn, David J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8904607/
https://www.ncbi.nlm.nih.gov/pubmed/35284839
http://dx.doi.org/10.1016/j.xpro.2022.101220
Descripción
Sumario:We present this protocol using a mouse model to assess the impact of early-life antibiotic exposure on mammalian lifespan and the composition of the gut microbiota over time. We describe longitudinal fecal sampling and health monitoring following early-life antibiotic exposure. We detail DNA extraction and 16S rRNA gene sequencing to longitudinally profile the composition of the fecal microbiota. Finally, we discuss how to address potential confounders such as the stochastic recolonization of the gut microbiota following antibiotic exposure. For complete details on the use and execution of this protocol, please refer to Lynn et al. (2021).

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