Protocol to assess the impact of early-life antibiotic exposure on murine longevity

We present this protocol using a mouse model to assess the impact of early-life antibiotic exposure on mammalian lifespan and the composition of the gut microbiota over time. We describe longitudinal fecal sampling and health monitoring following early-life antibiotic exposure. We detail DNA extract...

Descripción completa

Detalles Bibliográficos
Autores principales: Lynn, Miriam A., Ryan, Feargal J., Tee, Yee C., Benson, Saoirse C., Lynn, David J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8904607/
https://www.ncbi.nlm.nih.gov/pubmed/35284839
http://dx.doi.org/10.1016/j.xpro.2022.101220
_version_ 1784664991885950976
author Lynn, Miriam A.
Ryan, Feargal J.
Tee, Yee C.
Benson, Saoirse C.
Lynn, David J.
author_facet Lynn, Miriam A.
Ryan, Feargal J.
Tee, Yee C.
Benson, Saoirse C.
Lynn, David J.
author_sort Lynn, Miriam A.
collection PubMed
description We present this protocol using a mouse model to assess the impact of early-life antibiotic exposure on mammalian lifespan and the composition of the gut microbiota over time. We describe longitudinal fecal sampling and health monitoring following early-life antibiotic exposure. We detail DNA extraction and 16S rRNA gene sequencing to longitudinally profile the composition of the fecal microbiota. Finally, we discuss how to address potential confounders such as the stochastic recolonization of the gut microbiota following antibiotic exposure. For complete details on the use and execution of this protocol, please refer to Lynn et al. (2021).
format Online
Article
Text
id pubmed-8904607
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-89046072022-03-10 Protocol to assess the impact of early-life antibiotic exposure on murine longevity Lynn, Miriam A. Ryan, Feargal J. Tee, Yee C. Benson, Saoirse C. Lynn, David J. STAR Protoc Protocol We present this protocol using a mouse model to assess the impact of early-life antibiotic exposure on mammalian lifespan and the composition of the gut microbiota over time. We describe longitudinal fecal sampling and health monitoring following early-life antibiotic exposure. We detail DNA extraction and 16S rRNA gene sequencing to longitudinally profile the composition of the fecal microbiota. Finally, we discuss how to address potential confounders such as the stochastic recolonization of the gut microbiota following antibiotic exposure. For complete details on the use and execution of this protocol, please refer to Lynn et al. (2021). Elsevier 2022-03-05 /pmc/articles/PMC8904607/ /pubmed/35284839 http://dx.doi.org/10.1016/j.xpro.2022.101220 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Lynn, Miriam A.
Ryan, Feargal J.
Tee, Yee C.
Benson, Saoirse C.
Lynn, David J.
Protocol to assess the impact of early-life antibiotic exposure on murine longevity
title Protocol to assess the impact of early-life antibiotic exposure on murine longevity
title_full Protocol to assess the impact of early-life antibiotic exposure on murine longevity
title_fullStr Protocol to assess the impact of early-life antibiotic exposure on murine longevity
title_full_unstemmed Protocol to assess the impact of early-life antibiotic exposure on murine longevity
title_short Protocol to assess the impact of early-life antibiotic exposure on murine longevity
title_sort protocol to assess the impact of early-life antibiotic exposure on murine longevity
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8904607/
https://www.ncbi.nlm.nih.gov/pubmed/35284839
http://dx.doi.org/10.1016/j.xpro.2022.101220
work_keys_str_mv AT lynnmiriama protocoltoassesstheimpactofearlylifeantibioticexposureonmurinelongevity
AT ryanfeargalj protocoltoassesstheimpactofearlylifeantibioticexposureonmurinelongevity
AT teeyeec protocoltoassesstheimpactofearlylifeantibioticexposureonmurinelongevity
AT bensonsaoirsec protocoltoassesstheimpactofearlylifeantibioticexposureonmurinelongevity
AT lynndavidj protocoltoassesstheimpactofearlylifeantibioticexposureonmurinelongevity

Ejemplares similares