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Identification of the potential mechanism of Radix pueraria in colon cancer based on network pharmacology
Radix Puerariae (RP), a dry root of Pueraria lobata (Willd.) Ohwi, is used to treat a variety of diseases, including cancer. Several in vitro and in vivo studies have demonstrated the efficacy of RP in the treatment of colon cancer (CC). However, the biological mechanism of RP in the treatment of co...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8904787/ https://www.ncbi.nlm.nih.gov/pubmed/35260672 http://dx.doi.org/10.1038/s41598-022-07815-y |
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author | Li, Yi Zhang, Chunli Ma, Xiaohan Yang, Liuqing Ren, Huijun |
author_facet | Li, Yi Zhang, Chunli Ma, Xiaohan Yang, Liuqing Ren, Huijun |
author_sort | Li, Yi |
collection | PubMed |
description | Radix Puerariae (RP), a dry root of Pueraria lobata (Willd.) Ohwi, is used to treat a variety of diseases, including cancer. Several in vitro and in vivo studies have demonstrated the efficacy of RP in the treatment of colon cancer (CC). However, the biological mechanism of RP in the treatment of colon cancer remains unclear. In this study, the active component of RP and its potential molecular mechanism against CC were studied by network pharmacology and enrichment analysis. The methods adopted included screening active ingredients of Chinese medicine, predicting target genes of Chinese medicine and disease, constructing of a protein interaction network, and conducting GO and KEGG enrichment analysis. Finally, the results of network pharmacology were further validated by molecular docking experiments and cell experiments. Eight active constituents and 14 potential protein targets were screened from RP, including EGFR, JAK2 and SRC. The biological mechanism of RP against CC was analysed by studying the relationship between active components, targets, and enrichment pathways. These findings provide a basis for understanding the clinical application of RP in CC. |
format | Online Article Text |
id | pubmed-8904787 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-89047872022-03-10 Identification of the potential mechanism of Radix pueraria in colon cancer based on network pharmacology Li, Yi Zhang, Chunli Ma, Xiaohan Yang, Liuqing Ren, Huijun Sci Rep Article Radix Puerariae (RP), a dry root of Pueraria lobata (Willd.) Ohwi, is used to treat a variety of diseases, including cancer. Several in vitro and in vivo studies have demonstrated the efficacy of RP in the treatment of colon cancer (CC). However, the biological mechanism of RP in the treatment of colon cancer remains unclear. In this study, the active component of RP and its potential molecular mechanism against CC were studied by network pharmacology and enrichment analysis. The methods adopted included screening active ingredients of Chinese medicine, predicting target genes of Chinese medicine and disease, constructing of a protein interaction network, and conducting GO and KEGG enrichment analysis. Finally, the results of network pharmacology were further validated by molecular docking experiments and cell experiments. Eight active constituents and 14 potential protein targets were screened from RP, including EGFR, JAK2 and SRC. The biological mechanism of RP against CC was analysed by studying the relationship between active components, targets, and enrichment pathways. These findings provide a basis for understanding the clinical application of RP in CC. Nature Publishing Group UK 2022-03-08 /pmc/articles/PMC8904787/ /pubmed/35260672 http://dx.doi.org/10.1038/s41598-022-07815-y Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Li, Yi Zhang, Chunli Ma, Xiaohan Yang, Liuqing Ren, Huijun Identification of the potential mechanism of Radix pueraria in colon cancer based on network pharmacology |
title | Identification of the potential mechanism of Radix pueraria in colon cancer based on network pharmacology |
title_full | Identification of the potential mechanism of Radix pueraria in colon cancer based on network pharmacology |
title_fullStr | Identification of the potential mechanism of Radix pueraria in colon cancer based on network pharmacology |
title_full_unstemmed | Identification of the potential mechanism of Radix pueraria in colon cancer based on network pharmacology |
title_short | Identification of the potential mechanism of Radix pueraria in colon cancer based on network pharmacology |
title_sort | identification of the potential mechanism of radix pueraria in colon cancer based on network pharmacology |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8904787/ https://www.ncbi.nlm.nih.gov/pubmed/35260672 http://dx.doi.org/10.1038/s41598-022-07815-y |
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