Cargando…

Soil Properties Interacting With Microbial Metagenome in Decreasing CH(4) Emission From Seasonally Flooded Marshland Following Different Stages of Afforestation

Wetlands are the largest natural source of terrestrial CH(4) emissions. Afforestation can enhance soil CH(4) oxidation and decrease methanogenesis, yet the driving mechanisms leading to these effects remain unclear. We analyzed the structures of communities of methanogenic and methanotrophic microbe...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Qian, Tang, Jie, Angel, Roey, Wang, Dong, Hu, Xingyi, Gao, Shenghua, Zhang, Lei, Tang, Yuxi, Zhang, Xudong, Koide, Roger T., Yang, Haishui, Sun, Qixiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8905362/
https://www.ncbi.nlm.nih.gov/pubmed/35283824
http://dx.doi.org/10.3389/fmicb.2022.830019
_version_ 1784665169178132480
author Zhang, Qian
Tang, Jie
Angel, Roey
Wang, Dong
Hu, Xingyi
Gao, Shenghua
Zhang, Lei
Tang, Yuxi
Zhang, Xudong
Koide, Roger T.
Yang, Haishui
Sun, Qixiang
author_facet Zhang, Qian
Tang, Jie
Angel, Roey
Wang, Dong
Hu, Xingyi
Gao, Shenghua
Zhang, Lei
Tang, Yuxi
Zhang, Xudong
Koide, Roger T.
Yang, Haishui
Sun, Qixiang
author_sort Zhang, Qian
collection PubMed
description Wetlands are the largest natural source of terrestrial CH(4) emissions. Afforestation can enhance soil CH(4) oxidation and decrease methanogenesis, yet the driving mechanisms leading to these effects remain unclear. We analyzed the structures of communities of methanogenic and methanotrophic microbes, quantification of mcrA and pmoA genes, the soil microbial metagenome, soil properties and CH(4) fluxes in afforested and non-afforested areas in the marshland of the Yangtze River. Compared to the non-afforested land use types, net CH(4) emission decreased from bare land, natural vegetation and 5-year forest plantation and transitioned to net CH(4) sinks in the 10- and 20-year forest plantations. Both abundances of mcrA and pmoA genes decreased significantly with increasing plantation age. By combining random forest analysis and structural equation modeling, our results provide evidence for an important role of the abundance of functional genes related to methane production in explaining the net CH(4) flux in this ecosystem. The structures of methanogenic and methanotrophic microbial communities were of lower importance as explanatory factors than functional genes in terms of in situ CH(4) flux. We also found a substantial interaction between functional genes and soil properties in the control of CH(4) flux, particularly soil particle size. Our study provides empirical evidence that microbial community function has more explanatory power than taxonomic microbial community structure with respect to in situ CH(4) fluxes. This suggests that focusing on gene abundances obtained, e.g., through metagenomics or quantitative/digital PCR could be more effective than community profiling in predicting CH(4) fluxes, and such data should be considered for ecosystem modeling.
format Online
Article
Text
id pubmed-8905362
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-89053622022-03-10 Soil Properties Interacting With Microbial Metagenome in Decreasing CH(4) Emission From Seasonally Flooded Marshland Following Different Stages of Afforestation Zhang, Qian Tang, Jie Angel, Roey Wang, Dong Hu, Xingyi Gao, Shenghua Zhang, Lei Tang, Yuxi Zhang, Xudong Koide, Roger T. Yang, Haishui Sun, Qixiang Front Microbiol Microbiology Wetlands are the largest natural source of terrestrial CH(4) emissions. Afforestation can enhance soil CH(4) oxidation and decrease methanogenesis, yet the driving mechanisms leading to these effects remain unclear. We analyzed the structures of communities of methanogenic and methanotrophic microbes, quantification of mcrA and pmoA genes, the soil microbial metagenome, soil properties and CH(4) fluxes in afforested and non-afforested areas in the marshland of the Yangtze River. Compared to the non-afforested land use types, net CH(4) emission decreased from bare land, natural vegetation and 5-year forest plantation and transitioned to net CH(4) sinks in the 10- and 20-year forest plantations. Both abundances of mcrA and pmoA genes decreased significantly with increasing plantation age. By combining random forest analysis and structural equation modeling, our results provide evidence for an important role of the abundance of functional genes related to methane production in explaining the net CH(4) flux in this ecosystem. The structures of methanogenic and methanotrophic microbial communities were of lower importance as explanatory factors than functional genes in terms of in situ CH(4) flux. We also found a substantial interaction between functional genes and soil properties in the control of CH(4) flux, particularly soil particle size. Our study provides empirical evidence that microbial community function has more explanatory power than taxonomic microbial community structure with respect to in situ CH(4) fluxes. This suggests that focusing on gene abundances obtained, e.g., through metagenomics or quantitative/digital PCR could be more effective than community profiling in predicting CH(4) fluxes, and such data should be considered for ecosystem modeling. Frontiers Media S.A. 2022-02-23 /pmc/articles/PMC8905362/ /pubmed/35283824 http://dx.doi.org/10.3389/fmicb.2022.830019 Text en Copyright © 2022 Zhang, Tang, Angel, Wang, Hu, Gao, Zhang, Tang, Zhang, Koide, Yang and Sun. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Zhang, Qian
Tang, Jie
Angel, Roey
Wang, Dong
Hu, Xingyi
Gao, Shenghua
Zhang, Lei
Tang, Yuxi
Zhang, Xudong
Koide, Roger T.
Yang, Haishui
Sun, Qixiang
Soil Properties Interacting With Microbial Metagenome in Decreasing CH(4) Emission From Seasonally Flooded Marshland Following Different Stages of Afforestation
title Soil Properties Interacting With Microbial Metagenome in Decreasing CH(4) Emission From Seasonally Flooded Marshland Following Different Stages of Afforestation
title_full Soil Properties Interacting With Microbial Metagenome in Decreasing CH(4) Emission From Seasonally Flooded Marshland Following Different Stages of Afforestation
title_fullStr Soil Properties Interacting With Microbial Metagenome in Decreasing CH(4) Emission From Seasonally Flooded Marshland Following Different Stages of Afforestation
title_full_unstemmed Soil Properties Interacting With Microbial Metagenome in Decreasing CH(4) Emission From Seasonally Flooded Marshland Following Different Stages of Afforestation
title_short Soil Properties Interacting With Microbial Metagenome in Decreasing CH(4) Emission From Seasonally Flooded Marshland Following Different Stages of Afforestation
title_sort soil properties interacting with microbial metagenome in decreasing ch(4) emission from seasonally flooded marshland following different stages of afforestation
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8905362/
https://www.ncbi.nlm.nih.gov/pubmed/35283824
http://dx.doi.org/10.3389/fmicb.2022.830019
work_keys_str_mv AT zhangqian soilpropertiesinteractingwithmicrobialmetagenomeindecreasingch4emissionfromseasonallyfloodedmarshlandfollowingdifferentstagesofafforestation
AT tangjie soilpropertiesinteractingwithmicrobialmetagenomeindecreasingch4emissionfromseasonallyfloodedmarshlandfollowingdifferentstagesofafforestation
AT angelroey soilpropertiesinteractingwithmicrobialmetagenomeindecreasingch4emissionfromseasonallyfloodedmarshlandfollowingdifferentstagesofafforestation
AT wangdong soilpropertiesinteractingwithmicrobialmetagenomeindecreasingch4emissionfromseasonallyfloodedmarshlandfollowingdifferentstagesofafforestation
AT huxingyi soilpropertiesinteractingwithmicrobialmetagenomeindecreasingch4emissionfromseasonallyfloodedmarshlandfollowingdifferentstagesofafforestation
AT gaoshenghua soilpropertiesinteractingwithmicrobialmetagenomeindecreasingch4emissionfromseasonallyfloodedmarshlandfollowingdifferentstagesofafforestation
AT zhanglei soilpropertiesinteractingwithmicrobialmetagenomeindecreasingch4emissionfromseasonallyfloodedmarshlandfollowingdifferentstagesofafforestation
AT tangyuxi soilpropertiesinteractingwithmicrobialmetagenomeindecreasingch4emissionfromseasonallyfloodedmarshlandfollowingdifferentstagesofafforestation
AT zhangxudong soilpropertiesinteractingwithmicrobialmetagenomeindecreasingch4emissionfromseasonallyfloodedmarshlandfollowingdifferentstagesofafforestation
AT koiderogert soilpropertiesinteractingwithmicrobialmetagenomeindecreasingch4emissionfromseasonallyfloodedmarshlandfollowingdifferentstagesofafforestation
AT yanghaishui soilpropertiesinteractingwithmicrobialmetagenomeindecreasingch4emissionfromseasonallyfloodedmarshlandfollowingdifferentstagesofafforestation
AT sunqixiang soilpropertiesinteractingwithmicrobialmetagenomeindecreasingch4emissionfromseasonallyfloodedmarshlandfollowingdifferentstagesofafforestation