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DNA barcode reveals candidate species of Scinax and Ololygon (Anura: Hylidae) in Atlantic Forest
Molecular species delimitation methods are efficient tools to identify species, including the discovery of new taxa and cryptic organisms, thus being useful to biodiversity studies. In the present work, 16S mitochondrial sequences and cytochrome oxidase I (COI) were used to evaluate the richness of...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Sociedade Brasileira de Genética
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8905458/ https://www.ncbi.nlm.nih.gov/pubmed/35262169 http://dx.doi.org/10.1590/1678-4685-GMB-2021-0177 |
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author | Nogueira, Lídia Rodrigues, Luís Fernando da Silva Solé, Mirco Affonso, Paulo Roberto Antunes de Mello Siqueira, Sergio Sampaio, Iracilda |
author_facet | Nogueira, Lídia Rodrigues, Luís Fernando da Silva Solé, Mirco Affonso, Paulo Roberto Antunes de Mello Siqueira, Sergio Sampaio, Iracilda |
author_sort | Nogueira, Lídia |
collection | PubMed |
description | Molecular species delimitation methods are efficient tools to identify species, including the discovery of new taxa and cryptic organisms, thus being useful to biodiversity studies. In the present work, 16S mitochondrial sequences and cytochrome oxidase I (COI) were used to evaluate the richness of species in the genus Scinax and Ololygon from a biodiversity hotspot in Atlantic Forest. A total of 109 specimens formally belonging to eight species of Scinax and three species of Ololygon were collected in 13 localities along the state of Bahia (northeastern Brazil) and one site in Espírito Santo (southeastern Brazil). Of the Scinax species collected in this study, three were morphologically differentiated from other described species and identified as putative new species (Scinax sp.1, Scinax sp.2 and Scinax sp.3). The species delimitations were inferred using three different methods: ABGD, PTP and mPTP which allowed recognizing 11 Scinax species and five Ololygon species. Scinax sp. 1, Scinax sp. 2 and Scinax sp. 3, have been confirmed as new putative species and Ololygon argyreornata possibly contains cryptic species. We suggest additional studies, including morphological and bioacoustic data to validate these new putative species. |
format | Online Article Text |
id | pubmed-8905458 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Sociedade Brasileira de Genética |
record_format | MEDLINE/PubMed |
spelling | pubmed-89054582022-03-17 DNA barcode reveals candidate species of Scinax and Ololygon (Anura: Hylidae) in Atlantic Forest Nogueira, Lídia Rodrigues, Luís Fernando da Silva Solé, Mirco Affonso, Paulo Roberto Antunes de Mello Siqueira, Sergio Sampaio, Iracilda Genet Mol Biol Animal Genetics Molecular species delimitation methods are efficient tools to identify species, including the discovery of new taxa and cryptic organisms, thus being useful to biodiversity studies. In the present work, 16S mitochondrial sequences and cytochrome oxidase I (COI) were used to evaluate the richness of species in the genus Scinax and Ololygon from a biodiversity hotspot in Atlantic Forest. A total of 109 specimens formally belonging to eight species of Scinax and three species of Ololygon were collected in 13 localities along the state of Bahia (northeastern Brazil) and one site in Espírito Santo (southeastern Brazil). Of the Scinax species collected in this study, three were morphologically differentiated from other described species and identified as putative new species (Scinax sp.1, Scinax sp.2 and Scinax sp.3). The species delimitations were inferred using three different methods: ABGD, PTP and mPTP which allowed recognizing 11 Scinax species and five Ololygon species. Scinax sp. 1, Scinax sp. 2 and Scinax sp. 3, have been confirmed as new putative species and Ololygon argyreornata possibly contains cryptic species. We suggest additional studies, including morphological and bioacoustic data to validate these new putative species. Sociedade Brasileira de Genética 2022-03-07 /pmc/articles/PMC8905458/ /pubmed/35262169 http://dx.doi.org/10.1590/1678-4685-GMB-2021-0177 Text en https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License |
spellingShingle | Animal Genetics Nogueira, Lídia Rodrigues, Luís Fernando da Silva Solé, Mirco Affonso, Paulo Roberto Antunes de Mello Siqueira, Sergio Sampaio, Iracilda DNA barcode reveals candidate species of Scinax and Ololygon (Anura: Hylidae) in Atlantic Forest |
title | DNA barcode reveals candidate species of Scinax and Ololygon (Anura: Hylidae) in Atlantic Forest |
title_full | DNA barcode reveals candidate species of Scinax and Ololygon (Anura: Hylidae) in Atlantic Forest |
title_fullStr | DNA barcode reveals candidate species of Scinax and Ololygon (Anura: Hylidae) in Atlantic Forest |
title_full_unstemmed | DNA barcode reveals candidate species of Scinax and Ololygon (Anura: Hylidae) in Atlantic Forest |
title_short | DNA barcode reveals candidate species of Scinax and Ololygon (Anura: Hylidae) in Atlantic Forest |
title_sort | dna barcode reveals candidate species of scinax and ololygon (anura: hylidae) in atlantic forest |
topic | Animal Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8905458/ https://www.ncbi.nlm.nih.gov/pubmed/35262169 http://dx.doi.org/10.1590/1678-4685-GMB-2021-0177 |
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