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Ca(2+)-Permeable Channels/Ca(2+) Signaling in the Regulation of Ileal Na(+)/Gln Co-Transport in Mice
Oral glutamine (Gln) has been widely used in gastrointestinal (GI) clinical practice, but it is unclear if Ca(2+) regulates intestinal Gln transport, although both of them are essential nutrients for mammals. Chambers were used to determine Gln (25 mM)-induced I ( sc ) through Na(+)/Gln co-transport...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8905502/ https://www.ncbi.nlm.nih.gov/pubmed/35281933 http://dx.doi.org/10.3389/fphar.2022.816133 |
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author | Chu, Fenglan Wan, Hanxing Xiao, Weidong Dong, Hui Lü, Muhan |
author_facet | Chu, Fenglan Wan, Hanxing Xiao, Weidong Dong, Hui Lü, Muhan |
author_sort | Chu, Fenglan |
collection | PubMed |
description | Oral glutamine (Gln) has been widely used in gastrointestinal (GI) clinical practice, but it is unclear if Ca(2+) regulates intestinal Gln transport, although both of them are essential nutrients for mammals. Chambers were used to determine Gln (25 mM)-induced I ( sc ) through Na(+)/Gln co-transporters in the small intestine in the absence or the presence of selective activators or blockers of ion channels and transporters. Luminal but not serosal application of Gln induced marked intestinal I ( sc ), especially in the distal ileum. Lowering luminal Na(+) almost abolished the Gln-induced ileal I ( sc ), in which the calcium-sensitive receptor (CaSR) activation were not involved. Ca(2+) removal from both luminal and serosal sides of the ileum significantly reduced Gln- I ( sc ). Blocking either luminal Ca(2+) entry via the voltage-gated calcium channels (VGCC) or endoplasmic reticulum (ER) release via inositol 1,4,5-triphosphate receptor (IP(3)R) and ryanodine receptor (RyR) attenuated the Gln-induced ileal I ( sc ), Likewise, blocking serosal Ca(2+) entry via the store-operated Ca(2+) entry (SOCE), TRPV1/2 channels, and Na(+)/Ca(2+) exchangers (NCX) attenuated the Gln-induced ileal I ( sc ). In contrast, activating TRPV1/2 channels enhanced the Gln-induced ileal I ( sc ). We concluded that Ca(2+) signaling is critical for intestinal Gln transport, and multiple plasma membrane Ca(2+)-permeable channels and transporters play roles in this process. The Ca(2+) regulation of ileal Na(+)/Gln transport expands our understanding of intestinal nutrient uptake and may be significant in GI health and disease. |
format | Online Article Text |
id | pubmed-8905502 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-89055022022-03-10 Ca(2+)-Permeable Channels/Ca(2+) Signaling in the Regulation of Ileal Na(+)/Gln Co-Transport in Mice Chu, Fenglan Wan, Hanxing Xiao, Weidong Dong, Hui Lü, Muhan Front Pharmacol Pharmacology Oral glutamine (Gln) has been widely used in gastrointestinal (GI) clinical practice, but it is unclear if Ca(2+) regulates intestinal Gln transport, although both of them are essential nutrients for mammals. Chambers were used to determine Gln (25 mM)-induced I ( sc ) through Na(+)/Gln co-transporters in the small intestine in the absence or the presence of selective activators or blockers of ion channels and transporters. Luminal but not serosal application of Gln induced marked intestinal I ( sc ), especially in the distal ileum. Lowering luminal Na(+) almost abolished the Gln-induced ileal I ( sc ), in which the calcium-sensitive receptor (CaSR) activation were not involved. Ca(2+) removal from both luminal and serosal sides of the ileum significantly reduced Gln- I ( sc ). Blocking either luminal Ca(2+) entry via the voltage-gated calcium channels (VGCC) or endoplasmic reticulum (ER) release via inositol 1,4,5-triphosphate receptor (IP(3)R) and ryanodine receptor (RyR) attenuated the Gln-induced ileal I ( sc ), Likewise, blocking serosal Ca(2+) entry via the store-operated Ca(2+) entry (SOCE), TRPV1/2 channels, and Na(+)/Ca(2+) exchangers (NCX) attenuated the Gln-induced ileal I ( sc ). In contrast, activating TRPV1/2 channels enhanced the Gln-induced ileal I ( sc ). We concluded that Ca(2+) signaling is critical for intestinal Gln transport, and multiple plasma membrane Ca(2+)-permeable channels and transporters play roles in this process. The Ca(2+) regulation of ileal Na(+)/Gln transport expands our understanding of intestinal nutrient uptake and may be significant in GI health and disease. Frontiers Media S.A. 2022-02-23 /pmc/articles/PMC8905502/ /pubmed/35281933 http://dx.doi.org/10.3389/fphar.2022.816133 Text en Copyright © 2022 Chu, Wan, Xiao, Dong and Lü. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Pharmacology Chu, Fenglan Wan, Hanxing Xiao, Weidong Dong, Hui Lü, Muhan Ca(2+)-Permeable Channels/Ca(2+) Signaling in the Regulation of Ileal Na(+)/Gln Co-Transport in Mice |
title | Ca(2+)-Permeable Channels/Ca(2+) Signaling in the Regulation of Ileal Na(+)/Gln Co-Transport in Mice |
title_full | Ca(2+)-Permeable Channels/Ca(2+) Signaling in the Regulation of Ileal Na(+)/Gln Co-Transport in Mice |
title_fullStr | Ca(2+)-Permeable Channels/Ca(2+) Signaling in the Regulation of Ileal Na(+)/Gln Co-Transport in Mice |
title_full_unstemmed | Ca(2+)-Permeable Channels/Ca(2+) Signaling in the Regulation of Ileal Na(+)/Gln Co-Transport in Mice |
title_short | Ca(2+)-Permeable Channels/Ca(2+) Signaling in the Regulation of Ileal Na(+)/Gln Co-Transport in Mice |
title_sort | ca(2+)-permeable channels/ca(2+) signaling in the regulation of ileal na(+)/gln co-transport in mice |
topic | Pharmacology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8905502/ https://www.ncbi.nlm.nih.gov/pubmed/35281933 http://dx.doi.org/10.3389/fphar.2022.816133 |
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