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NLRP3 Is Involved in Neutrophil Mobilization in Experimental Periodontitis

The NLRP3 inflammasome is overexpressed in gingiva of periodontitis patients but its role remains unclear. In our study, we use a periodontitis mouse model of ligature, impregnated or not with Porphyromonas gingivalis, in WT or NLRP3 KO mice. After 28 days of induction, ligature alone provoked exace...

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Autores principales: Cheat, Banndith, Torrens, Coralie, Foda, Asmaa, Baroukh, Brigitte, Sadoine, Jeremy, Slimani, Lotfi, Witko-Sarsat, Véronique, Huck, Olivier, Gosset, Marjolaine, Bouchet, Jérôme
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8905524/
https://www.ncbi.nlm.nih.gov/pubmed/35281020
http://dx.doi.org/10.3389/fimmu.2022.839929
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author Cheat, Banndith
Torrens, Coralie
Foda, Asmaa
Baroukh, Brigitte
Sadoine, Jeremy
Slimani, Lotfi
Witko-Sarsat, Véronique
Huck, Olivier
Gosset, Marjolaine
Bouchet, Jérôme
author_facet Cheat, Banndith
Torrens, Coralie
Foda, Asmaa
Baroukh, Brigitte
Sadoine, Jeremy
Slimani, Lotfi
Witko-Sarsat, Véronique
Huck, Olivier
Gosset, Marjolaine
Bouchet, Jérôme
author_sort Cheat, Banndith
collection PubMed
description The NLRP3 inflammasome is overexpressed in gingiva of periodontitis patients but its role remains unclear. In our study, we use a periodontitis mouse model of ligature, impregnated or not with Porphyromonas gingivalis, in WT or NLRP3 KO mice. After 28 days of induction, ligature alone provoked exacerbated periodontal destruction in KO mice, compared to WT mice, with an increase in activated osteoclasts. No difference was observed at 14 days, suggesting that NLRP3 is involved in regulatory pathways that limit periodontitis. In contrast, in the presence of P. gingivalis, this protective effect of NLRP3 was not observed. Overexpression of NLRP3 in connective tissue of WT mice increased the local production of mature IL−1β, together with a dramatic mobilization of neutrophils, bipartitely distributed between the site of periodontitis induction and the alveolar bone crest. P. gingivalis enhanced the targeting of NLRP3-positive neutrophils to the alveolar bone crest, suggesting a role for this subpopulation in bone loss. Conversely, in NLRP3 KO mice, mature IL-1β expression was lower and almost no neutrophils were mobilized. Our study sheds new light on the role of NLRP3 in periodontitis by highlighting the ambiguous role of neutrophils, and P. gingivalis which affects NLRP3 functions.
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spelling pubmed-89055242022-03-10 NLRP3 Is Involved in Neutrophil Mobilization in Experimental Periodontitis Cheat, Banndith Torrens, Coralie Foda, Asmaa Baroukh, Brigitte Sadoine, Jeremy Slimani, Lotfi Witko-Sarsat, Véronique Huck, Olivier Gosset, Marjolaine Bouchet, Jérôme Front Immunol Immunology The NLRP3 inflammasome is overexpressed in gingiva of periodontitis patients but its role remains unclear. In our study, we use a periodontitis mouse model of ligature, impregnated or not with Porphyromonas gingivalis, in WT or NLRP3 KO mice. After 28 days of induction, ligature alone provoked exacerbated periodontal destruction in KO mice, compared to WT mice, with an increase in activated osteoclasts. No difference was observed at 14 days, suggesting that NLRP3 is involved in regulatory pathways that limit periodontitis. In contrast, in the presence of P. gingivalis, this protective effect of NLRP3 was not observed. Overexpression of NLRP3 in connective tissue of WT mice increased the local production of mature IL−1β, together with a dramatic mobilization of neutrophils, bipartitely distributed between the site of periodontitis induction and the alveolar bone crest. P. gingivalis enhanced the targeting of NLRP3-positive neutrophils to the alveolar bone crest, suggesting a role for this subpopulation in bone loss. Conversely, in NLRP3 KO mice, mature IL-1β expression was lower and almost no neutrophils were mobilized. Our study sheds new light on the role of NLRP3 in periodontitis by highlighting the ambiguous role of neutrophils, and P. gingivalis which affects NLRP3 functions. Frontiers Media S.A. 2022-02-23 /pmc/articles/PMC8905524/ /pubmed/35281020 http://dx.doi.org/10.3389/fimmu.2022.839929 Text en Copyright © 2022 Cheat, Torrens, Foda, Baroukh, Sadoine, Slimani, Witko-Sarsat, Huck, Gosset and Bouchet https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Cheat, Banndith
Torrens, Coralie
Foda, Asmaa
Baroukh, Brigitte
Sadoine, Jeremy
Slimani, Lotfi
Witko-Sarsat, Véronique
Huck, Olivier
Gosset, Marjolaine
Bouchet, Jérôme
NLRP3 Is Involved in Neutrophil Mobilization in Experimental Periodontitis
title NLRP3 Is Involved in Neutrophil Mobilization in Experimental Periodontitis
title_full NLRP3 Is Involved in Neutrophil Mobilization in Experimental Periodontitis
title_fullStr NLRP3 Is Involved in Neutrophil Mobilization in Experimental Periodontitis
title_full_unstemmed NLRP3 Is Involved in Neutrophil Mobilization in Experimental Periodontitis
title_short NLRP3 Is Involved in Neutrophil Mobilization in Experimental Periodontitis
title_sort nlrp3 is involved in neutrophil mobilization in experimental periodontitis
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8905524/
https://www.ncbi.nlm.nih.gov/pubmed/35281020
http://dx.doi.org/10.3389/fimmu.2022.839929
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