Dependence of click-SELEX performance on the nature and average number of modified nucleotides

The click-SELEX procedure enables the identification of nucleobase-modified aptamers in which chemical entities are introduced by a copper(i)-catalysed alkyne-azide ‘click’ reaction. Here we report on the impact of modified nucleobases on PCR conditions and the average amount of modified nucleobases...

Descripción completa

Detalles Bibliográficos
Autores principales: Siegl, Julia, Plückthun, Olga, Mayer, Günter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: RSC 2022
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8905535/
https://www.ncbi.nlm.nih.gov/pubmed/35359492
http://dx.doi.org/10.1039/d2cb00012a
_version_ 1784665208472469504
author Siegl, Julia
Plückthun, Olga
Mayer, Günter
author_facet Siegl, Julia
Plückthun, Olga
Mayer, Günter
author_sort Siegl, Julia
collection PubMed
description The click-SELEX procedure enables the identification of nucleobase-modified aptamers in which chemical entities are introduced by a copper(i)-catalysed alkyne-azide ‘click’ reaction. Here we report on the impact of modified nucleobases on PCR conditions and the average amount of modified nucleobases on click-SELEX performance. We demonstrate click-SELEX being strongly dependent on which and on how many modifications are used. However, when using C3-GFP the number of modifications did not impact the overall success of the selection procedure.
format Online
Article
Text
id pubmed-8905535
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher RSC
record_format MEDLINE/PubMed
spelling pubmed-89055352022-03-30 Dependence of click-SELEX performance on the nature and average number of modified nucleotides Siegl, Julia Plückthun, Olga Mayer, Günter RSC Chem Biol Chemistry The click-SELEX procedure enables the identification of nucleobase-modified aptamers in which chemical entities are introduced by a copper(i)-catalysed alkyne-azide ‘click’ reaction. Here we report on the impact of modified nucleobases on PCR conditions and the average amount of modified nucleobases on click-SELEX performance. We demonstrate click-SELEX being strongly dependent on which and on how many modifications are used. However, when using C3-GFP the number of modifications did not impact the overall success of the selection procedure. RSC 2022-02-21 /pmc/articles/PMC8905535/ /pubmed/35359492 http://dx.doi.org/10.1039/d2cb00012a Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Siegl, Julia
Plückthun, Olga
Mayer, Günter
Dependence of click-SELEX performance on the nature and average number of modified nucleotides
title Dependence of click-SELEX performance on the nature and average number of modified nucleotides
title_full Dependence of click-SELEX performance on the nature and average number of modified nucleotides
title_fullStr Dependence of click-SELEX performance on the nature and average number of modified nucleotides
title_full_unstemmed Dependence of click-SELEX performance on the nature and average number of modified nucleotides
title_short Dependence of click-SELEX performance on the nature and average number of modified nucleotides
title_sort dependence of click-selex performance on the nature and average number of modified nucleotides
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8905535/
https://www.ncbi.nlm.nih.gov/pubmed/35359492
http://dx.doi.org/10.1039/d2cb00012a
work_keys_str_mv AT siegljulia dependenceofclickselexperformanceonthenatureandaveragenumberofmodifiednucleotides
AT pluckthunolga dependenceofclickselexperformanceonthenatureandaveragenumberofmodifiednucleotides
AT mayergunter dependenceofclickselexperformanceonthenatureandaveragenumberofmodifiednucleotides

Ejemplares similares