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Chemogenetics of cell surface receptors: beyond genetic and pharmacological approaches

Cell surface receptors transmit extracellular information into cells. Spatiotemporal regulation of receptor signaling is crucial for cellular functions, and dysregulation of signaling causes various diseases. Thus, it is highly desired to control receptor functions with high spatial and/or temporal...

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Detalles Bibliográficos
Autores principales: Miura, Yuta, Senoo, Akinobu, Doura, Tomohiro, Kiyonaka, Shigeki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: RSC 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8905536/
https://www.ncbi.nlm.nih.gov/pubmed/35359495
http://dx.doi.org/10.1039/d1cb00195g
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author Miura, Yuta
Senoo, Akinobu
Doura, Tomohiro
Kiyonaka, Shigeki
author_facet Miura, Yuta
Senoo, Akinobu
Doura, Tomohiro
Kiyonaka, Shigeki
author_sort Miura, Yuta
collection PubMed
description Cell surface receptors transmit extracellular information into cells. Spatiotemporal regulation of receptor signaling is crucial for cellular functions, and dysregulation of signaling causes various diseases. Thus, it is highly desired to control receptor functions with high spatial and/or temporal resolution. Conventionally, genetic engineering or chemical ligands have been used to control receptor functions in cells. As the alternative, chemogenetics has been proposed, in which target proteins are genetically engineered to interact with a designed chemical partner with high selectivity. The engineered receptor dissects the function of one receptor member among a highly homologous receptor family in a cell-specific manner. Notably, some chemogenetic strategies have been used to reveal the receptor signaling of target cells in living animals. In this review, we summarize the developing chemogenetic methods of transmembrane receptors for cell-specific regulation of receptor signaling. We also discuss the prospects of chemogenetics for clinical applications.
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spelling pubmed-89055362022-03-30 Chemogenetics of cell surface receptors: beyond genetic and pharmacological approaches Miura, Yuta Senoo, Akinobu Doura, Tomohiro Kiyonaka, Shigeki RSC Chem Biol Chemistry Cell surface receptors transmit extracellular information into cells. Spatiotemporal regulation of receptor signaling is crucial for cellular functions, and dysregulation of signaling causes various diseases. Thus, it is highly desired to control receptor functions with high spatial and/or temporal resolution. Conventionally, genetic engineering or chemical ligands have been used to control receptor functions in cells. As the alternative, chemogenetics has been proposed, in which target proteins are genetically engineered to interact with a designed chemical partner with high selectivity. The engineered receptor dissects the function of one receptor member among a highly homologous receptor family in a cell-specific manner. Notably, some chemogenetic strategies have been used to reveal the receptor signaling of target cells in living animals. In this review, we summarize the developing chemogenetic methods of transmembrane receptors for cell-specific regulation of receptor signaling. We also discuss the prospects of chemogenetics for clinical applications. RSC 2022-01-27 /pmc/articles/PMC8905536/ /pubmed/35359495 http://dx.doi.org/10.1039/d1cb00195g Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Miura, Yuta
Senoo, Akinobu
Doura, Tomohiro
Kiyonaka, Shigeki
Chemogenetics of cell surface receptors: beyond genetic and pharmacological approaches
title Chemogenetics of cell surface receptors: beyond genetic and pharmacological approaches
title_full Chemogenetics of cell surface receptors: beyond genetic and pharmacological approaches
title_fullStr Chemogenetics of cell surface receptors: beyond genetic and pharmacological approaches
title_full_unstemmed Chemogenetics of cell surface receptors: beyond genetic and pharmacological approaches
title_short Chemogenetics of cell surface receptors: beyond genetic and pharmacological approaches
title_sort chemogenetics of cell surface receptors: beyond genetic and pharmacological approaches
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8905536/
https://www.ncbi.nlm.nih.gov/pubmed/35359495
http://dx.doi.org/10.1039/d1cb00195g
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