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lncRNA USP30-AS1 sponges miR-765 and modulates the progression of colon cancer

BACKGROUND: The incidence and mortality of colon cancer is increasing recently. It is necessary to identify effective biomarkers for the progression and prognosis of colon cancer. To assess the potential of lncRNA USP30-AS1 (USP30-AS1) in serving as the biomarker of colon cancer and unearth the unde...

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Autores principales: Li, Chengren, Liang, Xu, Liu, Yongguang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8905834/
https://www.ncbi.nlm.nih.gov/pubmed/35260141
http://dx.doi.org/10.1186/s12957-022-02529-x
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author Li, Chengren
Liang, Xu
Liu, Yongguang
author_facet Li, Chengren
Liang, Xu
Liu, Yongguang
author_sort Li, Chengren
collection PubMed
description BACKGROUND: The incidence and mortality of colon cancer is increasing recently. It is necessary to identify effective biomarkers for the progression and prognosis of colon cancer. To assess the potential of lncRNA USP30-AS1 (USP30-AS1) in serving as the biomarker of colon cancer and unearth the underlying mechanism. METHODS: There were 123 colon cancer patients enrolled. The expression of USP30-AS1 was evaluated with PCR in tissue and cell samples. The clinical significance of USP30-AS1 was assessed with a series of statistical methods, while the CCK8 and Transwell assay were conducted to estimate its biological effect on the colon cancer cellular processes. In mechanism, the interaction of USP30-AS1 with miR-765 was evaluated with the dual-luciferase reporter assay. RESULTS: In colon cancer tissues, the USP30-AS1 downregulation and the miR-765 upregulation were observed, and there was a negative correlation between the USP30-AS1 expression level and the miR-765 expression level. The downregulation of USP30-AS1 related to the malignant progression and served as an adverse prognostic indicator of colon cancer. The overexpression of USP30-AS1 dramatically suppressed colon cancer cellular processes, which was alleviated by miR-765. CONCLUSIONS: USP30-AS1 predicts the malignancy and prognosis of colon cancer patients. USP30-AS1 suppressed the progression of colon cancer through modulating miR-765.
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spelling pubmed-89058342022-03-18 lncRNA USP30-AS1 sponges miR-765 and modulates the progression of colon cancer Li, Chengren Liang, Xu Liu, Yongguang World J Surg Oncol Research BACKGROUND: The incidence and mortality of colon cancer is increasing recently. It is necessary to identify effective biomarkers for the progression and prognosis of colon cancer. To assess the potential of lncRNA USP30-AS1 (USP30-AS1) in serving as the biomarker of colon cancer and unearth the underlying mechanism. METHODS: There were 123 colon cancer patients enrolled. The expression of USP30-AS1 was evaluated with PCR in tissue and cell samples. The clinical significance of USP30-AS1 was assessed with a series of statistical methods, while the CCK8 and Transwell assay were conducted to estimate its biological effect on the colon cancer cellular processes. In mechanism, the interaction of USP30-AS1 with miR-765 was evaluated with the dual-luciferase reporter assay. RESULTS: In colon cancer tissues, the USP30-AS1 downregulation and the miR-765 upregulation were observed, and there was a negative correlation between the USP30-AS1 expression level and the miR-765 expression level. The downregulation of USP30-AS1 related to the malignant progression and served as an adverse prognostic indicator of colon cancer. The overexpression of USP30-AS1 dramatically suppressed colon cancer cellular processes, which was alleviated by miR-765. CONCLUSIONS: USP30-AS1 predicts the malignancy and prognosis of colon cancer patients. USP30-AS1 suppressed the progression of colon cancer through modulating miR-765. BioMed Central 2022-03-08 /pmc/articles/PMC8905834/ /pubmed/35260141 http://dx.doi.org/10.1186/s12957-022-02529-x Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Li, Chengren
Liang, Xu
Liu, Yongguang
lncRNA USP30-AS1 sponges miR-765 and modulates the progression of colon cancer
title lncRNA USP30-AS1 sponges miR-765 and modulates the progression of colon cancer
title_full lncRNA USP30-AS1 sponges miR-765 and modulates the progression of colon cancer
title_fullStr lncRNA USP30-AS1 sponges miR-765 and modulates the progression of colon cancer
title_full_unstemmed lncRNA USP30-AS1 sponges miR-765 and modulates the progression of colon cancer
title_short lncRNA USP30-AS1 sponges miR-765 and modulates the progression of colon cancer
title_sort lncrna usp30-as1 sponges mir-765 and modulates the progression of colon cancer
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8905834/
https://www.ncbi.nlm.nih.gov/pubmed/35260141
http://dx.doi.org/10.1186/s12957-022-02529-x
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