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Recent advances and application of whole genome amplification in molecular diagnosis and medicine
Whole genome amplification (WGA) is a technology for non‐selective amplification of the whole genome sequence, first appearing in 1992. Its primary purpose is to amplify and reflect the whole genome of trace tissues and single cells without sequence bias and to provide sufficient DNA template for su...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8906466/ https://www.ncbi.nlm.nih.gov/pubmed/35281794 http://dx.doi.org/10.1002/mco2.116 |
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author | Wang, Xiaoyu Liu, Yapeng Liu, Hongna Pan, Wenjing Ren, Jie Zheng, Xiangming Tan, Yimin Chen, Zhu Deng, Yan He, Nongyue Chen, Hui Li, Song |
author_facet | Wang, Xiaoyu Liu, Yapeng Liu, Hongna Pan, Wenjing Ren, Jie Zheng, Xiangming Tan, Yimin Chen, Zhu Deng, Yan He, Nongyue Chen, Hui Li, Song |
author_sort | Wang, Xiaoyu |
collection | PubMed |
description | Whole genome amplification (WGA) is a technology for non‐selective amplification of the whole genome sequence, first appearing in 1992. Its primary purpose is to amplify and reflect the whole genome of trace tissues and single cells without sequence bias and to provide sufficient DNA template for subsequent multigene and multilocus analysis, along with comprehensive genome research. WGA provides a method to obtain a large amount of genetic information from a small amount of DNA and provides a valuable tool for preserving limited samples in molecular biology. WGA technology is especially suitable for forensic identification and genetic disease research, along with new technologies such as next‐generation sequencing (NGS). In addition, WGA is also widely used in single‐cell sequencing. Due to the small amount of DNA in a single cell, it is often unable to meet the amount of samples needed for sequencing, so WGA is generally used to achieve the amplification of trace samples. This paper reviews WGA methods based on different principles, summarizes both amplification principle and amplification quality, and discusses the application prospects and challenges of WGA technology in molecular diagnosis and medicine. |
format | Online Article Text |
id | pubmed-8906466 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-89064662022-03-10 Recent advances and application of whole genome amplification in molecular diagnosis and medicine Wang, Xiaoyu Liu, Yapeng Liu, Hongna Pan, Wenjing Ren, Jie Zheng, Xiangming Tan, Yimin Chen, Zhu Deng, Yan He, Nongyue Chen, Hui Li, Song MedComm (2020) Reviews Whole genome amplification (WGA) is a technology for non‐selective amplification of the whole genome sequence, first appearing in 1992. Its primary purpose is to amplify and reflect the whole genome of trace tissues and single cells without sequence bias and to provide sufficient DNA template for subsequent multigene and multilocus analysis, along with comprehensive genome research. WGA provides a method to obtain a large amount of genetic information from a small amount of DNA and provides a valuable tool for preserving limited samples in molecular biology. WGA technology is especially suitable for forensic identification and genetic disease research, along with new technologies such as next‐generation sequencing (NGS). In addition, WGA is also widely used in single‐cell sequencing. Due to the small amount of DNA in a single cell, it is often unable to meet the amount of samples needed for sequencing, so WGA is generally used to achieve the amplification of trace samples. This paper reviews WGA methods based on different principles, summarizes both amplification principle and amplification quality, and discusses the application prospects and challenges of WGA technology in molecular diagnosis and medicine. John Wiley and Sons Inc. 2022-02-03 /pmc/articles/PMC8906466/ /pubmed/35281794 http://dx.doi.org/10.1002/mco2.116 Text en © 2022 The Authors. MedComm published by Sichuan International Medical Exchange & Promotion Association (SCIMEA) and John Wiley & Sons Australia, Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Reviews Wang, Xiaoyu Liu, Yapeng Liu, Hongna Pan, Wenjing Ren, Jie Zheng, Xiangming Tan, Yimin Chen, Zhu Deng, Yan He, Nongyue Chen, Hui Li, Song Recent advances and application of whole genome amplification in molecular diagnosis and medicine |
title | Recent advances and application of whole genome amplification in molecular diagnosis and medicine |
title_full | Recent advances and application of whole genome amplification in molecular diagnosis and medicine |
title_fullStr | Recent advances and application of whole genome amplification in molecular diagnosis and medicine |
title_full_unstemmed | Recent advances and application of whole genome amplification in molecular diagnosis and medicine |
title_short | Recent advances and application of whole genome amplification in molecular diagnosis and medicine |
title_sort | recent advances and application of whole genome amplification in molecular diagnosis and medicine |
topic | Reviews |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8906466/ https://www.ncbi.nlm.nih.gov/pubmed/35281794 http://dx.doi.org/10.1002/mco2.116 |
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