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Crystal structure of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) frameshifting pseudoknot

SARS-CoV-2 produces two long viral protein precursors from one open reading frame using a highly conserved RNA pseudoknot that enhances programmed −1 ribosomal frameshifting. The 1.3 Å-resolution X-ray structure of the pseudoknot reveals three coaxially stacked helices buttressed by idiosyncratic ba...

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Autores principales: Jones, Christopher P., Ferré-D'Amaré, Adrian R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8906546/
https://www.ncbi.nlm.nih.gov/pubmed/34845084
http://dx.doi.org/10.1261/rna.078825.121
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author Jones, Christopher P.
Ferré-D'Amaré, Adrian R.
author_facet Jones, Christopher P.
Ferré-D'Amaré, Adrian R.
author_sort Jones, Christopher P.
collection PubMed
description SARS-CoV-2 produces two long viral protein precursors from one open reading frame using a highly conserved RNA pseudoknot that enhances programmed −1 ribosomal frameshifting. The 1.3 Å-resolution X-ray structure of the pseudoknot reveals three coaxially stacked helices buttressed by idiosyncratic base triples from loop residues. This structure represents a frameshift-stimulating state that must be deformed by the ribosome and exhibits base-triple-adjacent pockets that could be targeted by future small-molecule therapeutics.
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spelling pubmed-89065462023-02-01 Crystal structure of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) frameshifting pseudoknot Jones, Christopher P. Ferré-D'Amaré, Adrian R. RNA Article SARS-CoV-2 produces two long viral protein precursors from one open reading frame using a highly conserved RNA pseudoknot that enhances programmed −1 ribosomal frameshifting. The 1.3 Å-resolution X-ray structure of the pseudoknot reveals three coaxially stacked helices buttressed by idiosyncratic base triples from loop residues. This structure represents a frameshift-stimulating state that must be deformed by the ribosome and exhibits base-triple-adjacent pockets that could be targeted by future small-molecule therapeutics. Cold Spring Harbor Laboratory Press 2022-02 /pmc/articles/PMC8906546/ /pubmed/34845084 http://dx.doi.org/10.1261/rna.078825.121 Text en Published by Cold Spring Harbor Laboratory Press for the RNA Society https://creativecommons.org/licenses/by-nc/4.0/This is a work of the US Government.
spellingShingle Article
Jones, Christopher P.
Ferré-D'Amaré, Adrian R.
Crystal structure of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) frameshifting pseudoknot
title Crystal structure of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) frameshifting pseudoknot
title_full Crystal structure of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) frameshifting pseudoknot
title_fullStr Crystal structure of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) frameshifting pseudoknot
title_full_unstemmed Crystal structure of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) frameshifting pseudoknot
title_short Crystal structure of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) frameshifting pseudoknot
title_sort crystal structure of the severe acute respiratory syndrome coronavirus 2 (sars-cov-2) frameshifting pseudoknot
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8906546/
https://www.ncbi.nlm.nih.gov/pubmed/34845084
http://dx.doi.org/10.1261/rna.078825.121
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