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Crystal structure of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) frameshifting pseudoknot
SARS-CoV-2 produces two long viral protein precursors from one open reading frame using a highly conserved RNA pseudoknot that enhances programmed −1 ribosomal frameshifting. The 1.3 Å-resolution X-ray structure of the pseudoknot reveals three coaxially stacked helices buttressed by idiosyncratic ba...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8906546/ https://www.ncbi.nlm.nih.gov/pubmed/34845084 http://dx.doi.org/10.1261/rna.078825.121 |
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author | Jones, Christopher P. Ferré-D'Amaré, Adrian R. |
author_facet | Jones, Christopher P. Ferré-D'Amaré, Adrian R. |
author_sort | Jones, Christopher P. |
collection | PubMed |
description | SARS-CoV-2 produces two long viral protein precursors from one open reading frame using a highly conserved RNA pseudoknot that enhances programmed −1 ribosomal frameshifting. The 1.3 Å-resolution X-ray structure of the pseudoknot reveals three coaxially stacked helices buttressed by idiosyncratic base triples from loop residues. This structure represents a frameshift-stimulating state that must be deformed by the ribosome and exhibits base-triple-adjacent pockets that could be targeted by future small-molecule therapeutics. |
format | Online Article Text |
id | pubmed-8906546 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Cold Spring Harbor Laboratory Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-89065462023-02-01 Crystal structure of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) frameshifting pseudoknot Jones, Christopher P. Ferré-D'Amaré, Adrian R. RNA Article SARS-CoV-2 produces two long viral protein precursors from one open reading frame using a highly conserved RNA pseudoknot that enhances programmed −1 ribosomal frameshifting. The 1.3 Å-resolution X-ray structure of the pseudoknot reveals three coaxially stacked helices buttressed by idiosyncratic base triples from loop residues. This structure represents a frameshift-stimulating state that must be deformed by the ribosome and exhibits base-triple-adjacent pockets that could be targeted by future small-molecule therapeutics. Cold Spring Harbor Laboratory Press 2022-02 /pmc/articles/PMC8906546/ /pubmed/34845084 http://dx.doi.org/10.1261/rna.078825.121 Text en Published by Cold Spring Harbor Laboratory Press for the RNA Society https://creativecommons.org/licenses/by-nc/4.0/This is a work of the US Government. |
spellingShingle | Article Jones, Christopher P. Ferré-D'Amaré, Adrian R. Crystal structure of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) frameshifting pseudoknot |
title | Crystal structure of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) frameshifting pseudoknot |
title_full | Crystal structure of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) frameshifting pseudoknot |
title_fullStr | Crystal structure of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) frameshifting pseudoknot |
title_full_unstemmed | Crystal structure of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) frameshifting pseudoknot |
title_short | Crystal structure of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) frameshifting pseudoknot |
title_sort | crystal structure of the severe acute respiratory syndrome coronavirus 2 (sars-cov-2) frameshifting pseudoknot |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8906546/ https://www.ncbi.nlm.nih.gov/pubmed/34845084 http://dx.doi.org/10.1261/rna.078825.121 |
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