Cargando…
Neuroanatomical abnormalities in a nonhuman primate model of congenital Zika virus infection
We evaluated neuropathological consequences of fetal ZIKV exposure in rhesus monkeys, a translatable animal model for human neural development, by carrying out quantitative neuroanatomical analyses of the nearly full-term brains of fetuses infected with ZIKV and procedure-matched controls. For each...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8906804/ https://www.ncbi.nlm.nih.gov/pubmed/35261339 http://dx.doi.org/10.7554/eLife.64734 |
_version_ | 1784665466370785280 |
---|---|
author | Beckman, Danielle Seelke, Adele MH Bennett, Jeffrey Dougherty, Paige Van Rompay, Koen KA Keesler, Rebekah Pesavento, Patricia A Coffey, Lark LA Morrison, John H Bliss-Moreau, Eliza |
author_facet | Beckman, Danielle Seelke, Adele MH Bennett, Jeffrey Dougherty, Paige Van Rompay, Koen KA Keesler, Rebekah Pesavento, Patricia A Coffey, Lark LA Morrison, John H Bliss-Moreau, Eliza |
author_sort | Beckman, Danielle |
collection | PubMed |
description | We evaluated neuropathological consequences of fetal ZIKV exposure in rhesus monkeys, a translatable animal model for human neural development, by carrying out quantitative neuroanatomical analyses of the nearly full-term brains of fetuses infected with ZIKV and procedure-matched controls. For each animal, a complete cerebral hemisphere was evaluated using immunohistochemical (IHC) and neuroanatomical techniques to detect virus, identify affected cell types, and evaluate gross neuroanatomical abnormalities. IHC staining revealed the presence of ZIKV in the frontal lobe, which contained activated microglia and showed increased apoptosis of immature neurons. ZIKV-infected animals exhibited macrostructural changes within the visual pathway. Regional differences tracked with the developmental timing of the brain, suggesting inflammatory processes related to viral infiltration swept through the cortex, followed by a wave of cell death resulting in morphological changes. These findings may help explain why some infants born with normal sized heads during the ZIKV epidemic manifest developmental challenges as they age. |
format | Online Article Text |
id | pubmed-8906804 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-89068042022-03-10 Neuroanatomical abnormalities in a nonhuman primate model of congenital Zika virus infection Beckman, Danielle Seelke, Adele MH Bennett, Jeffrey Dougherty, Paige Van Rompay, Koen KA Keesler, Rebekah Pesavento, Patricia A Coffey, Lark LA Morrison, John H Bliss-Moreau, Eliza eLife Microbiology and Infectious Disease We evaluated neuropathological consequences of fetal ZIKV exposure in rhesus monkeys, a translatable animal model for human neural development, by carrying out quantitative neuroanatomical analyses of the nearly full-term brains of fetuses infected with ZIKV and procedure-matched controls. For each animal, a complete cerebral hemisphere was evaluated using immunohistochemical (IHC) and neuroanatomical techniques to detect virus, identify affected cell types, and evaluate gross neuroanatomical abnormalities. IHC staining revealed the presence of ZIKV in the frontal lobe, which contained activated microglia and showed increased apoptosis of immature neurons. ZIKV-infected animals exhibited macrostructural changes within the visual pathway. Regional differences tracked with the developmental timing of the brain, suggesting inflammatory processes related to viral infiltration swept through the cortex, followed by a wave of cell death resulting in morphological changes. These findings may help explain why some infants born with normal sized heads during the ZIKV epidemic manifest developmental challenges as they age. eLife Sciences Publications, Ltd 2022-03-09 /pmc/articles/PMC8906804/ /pubmed/35261339 http://dx.doi.org/10.7554/eLife.64734 Text en © 2022, Beckman et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Microbiology and Infectious Disease Beckman, Danielle Seelke, Adele MH Bennett, Jeffrey Dougherty, Paige Van Rompay, Koen KA Keesler, Rebekah Pesavento, Patricia A Coffey, Lark LA Morrison, John H Bliss-Moreau, Eliza Neuroanatomical abnormalities in a nonhuman primate model of congenital Zika virus infection |
title | Neuroanatomical abnormalities in a nonhuman primate model of congenital Zika virus infection |
title_full | Neuroanatomical abnormalities in a nonhuman primate model of congenital Zika virus infection |
title_fullStr | Neuroanatomical abnormalities in a nonhuman primate model of congenital Zika virus infection |
title_full_unstemmed | Neuroanatomical abnormalities in a nonhuman primate model of congenital Zika virus infection |
title_short | Neuroanatomical abnormalities in a nonhuman primate model of congenital Zika virus infection |
title_sort | neuroanatomical abnormalities in a nonhuman primate model of congenital zika virus infection |
topic | Microbiology and Infectious Disease |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8906804/ https://www.ncbi.nlm.nih.gov/pubmed/35261339 http://dx.doi.org/10.7554/eLife.64734 |
work_keys_str_mv | AT beckmandanielle neuroanatomicalabnormalitiesinanonhumanprimatemodelofcongenitalzikavirusinfection AT seelkeadelemh neuroanatomicalabnormalitiesinanonhumanprimatemodelofcongenitalzikavirusinfection AT bennettjeffrey neuroanatomicalabnormalitiesinanonhumanprimatemodelofcongenitalzikavirusinfection AT doughertypaige neuroanatomicalabnormalitiesinanonhumanprimatemodelofcongenitalzikavirusinfection AT vanrompaykoenka neuroanatomicalabnormalitiesinanonhumanprimatemodelofcongenitalzikavirusinfection AT keeslerrebekah neuroanatomicalabnormalitiesinanonhumanprimatemodelofcongenitalzikavirusinfection AT pesaventopatriciaa neuroanatomicalabnormalitiesinanonhumanprimatemodelofcongenitalzikavirusinfection AT coffeylarkla neuroanatomicalabnormalitiesinanonhumanprimatemodelofcongenitalzikavirusinfection AT morrisonjohnh neuroanatomicalabnormalitiesinanonhumanprimatemodelofcongenitalzikavirusinfection AT blissmoreaueliza neuroanatomicalabnormalitiesinanonhumanprimatemodelofcongenitalzikavirusinfection |