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Neuroanatomical abnormalities in a nonhuman primate model of congenital Zika virus infection

We evaluated neuropathological consequences of fetal ZIKV exposure in rhesus monkeys, a translatable animal model for human neural development, by carrying out quantitative neuroanatomical analyses of the nearly full-term brains of fetuses infected with ZIKV and procedure-matched controls. For each...

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Autores principales: Beckman, Danielle, Seelke, Adele MH, Bennett, Jeffrey, Dougherty, Paige, Van Rompay, Koen KA, Keesler, Rebekah, Pesavento, Patricia A, Coffey, Lark LA, Morrison, John H, Bliss-Moreau, Eliza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8906804/
https://www.ncbi.nlm.nih.gov/pubmed/35261339
http://dx.doi.org/10.7554/eLife.64734
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author Beckman, Danielle
Seelke, Adele MH
Bennett, Jeffrey
Dougherty, Paige
Van Rompay, Koen KA
Keesler, Rebekah
Pesavento, Patricia A
Coffey, Lark LA
Morrison, John H
Bliss-Moreau, Eliza
author_facet Beckman, Danielle
Seelke, Adele MH
Bennett, Jeffrey
Dougherty, Paige
Van Rompay, Koen KA
Keesler, Rebekah
Pesavento, Patricia A
Coffey, Lark LA
Morrison, John H
Bliss-Moreau, Eliza
author_sort Beckman, Danielle
collection PubMed
description We evaluated neuropathological consequences of fetal ZIKV exposure in rhesus monkeys, a translatable animal model for human neural development, by carrying out quantitative neuroanatomical analyses of the nearly full-term brains of fetuses infected with ZIKV and procedure-matched controls. For each animal, a complete cerebral hemisphere was evaluated using immunohistochemical (IHC) and neuroanatomical techniques to detect virus, identify affected cell types, and evaluate gross neuroanatomical abnormalities. IHC staining revealed the presence of ZIKV in the frontal lobe, which contained activated microglia and showed increased apoptosis of immature neurons. ZIKV-infected animals exhibited macrostructural changes within the visual pathway. Regional differences tracked with the developmental timing of the brain, suggesting inflammatory processes related to viral infiltration swept through the cortex, followed by a wave of cell death resulting in morphological changes. These findings may help explain why some infants born with normal sized heads during the ZIKV epidemic manifest developmental challenges as they age.
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spelling pubmed-89068042022-03-10 Neuroanatomical abnormalities in a nonhuman primate model of congenital Zika virus infection Beckman, Danielle Seelke, Adele MH Bennett, Jeffrey Dougherty, Paige Van Rompay, Koen KA Keesler, Rebekah Pesavento, Patricia A Coffey, Lark LA Morrison, John H Bliss-Moreau, Eliza eLife Microbiology and Infectious Disease We evaluated neuropathological consequences of fetal ZIKV exposure in rhesus monkeys, a translatable animal model for human neural development, by carrying out quantitative neuroanatomical analyses of the nearly full-term brains of fetuses infected with ZIKV and procedure-matched controls. For each animal, a complete cerebral hemisphere was evaluated using immunohistochemical (IHC) and neuroanatomical techniques to detect virus, identify affected cell types, and evaluate gross neuroanatomical abnormalities. IHC staining revealed the presence of ZIKV in the frontal lobe, which contained activated microglia and showed increased apoptosis of immature neurons. ZIKV-infected animals exhibited macrostructural changes within the visual pathway. Regional differences tracked with the developmental timing of the brain, suggesting inflammatory processes related to viral infiltration swept through the cortex, followed by a wave of cell death resulting in morphological changes. These findings may help explain why some infants born with normal sized heads during the ZIKV epidemic manifest developmental challenges as they age. eLife Sciences Publications, Ltd 2022-03-09 /pmc/articles/PMC8906804/ /pubmed/35261339 http://dx.doi.org/10.7554/eLife.64734 Text en © 2022, Beckman et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Microbiology and Infectious Disease
Beckman, Danielle
Seelke, Adele MH
Bennett, Jeffrey
Dougherty, Paige
Van Rompay, Koen KA
Keesler, Rebekah
Pesavento, Patricia A
Coffey, Lark LA
Morrison, John H
Bliss-Moreau, Eliza
Neuroanatomical abnormalities in a nonhuman primate model of congenital Zika virus infection
title Neuroanatomical abnormalities in a nonhuman primate model of congenital Zika virus infection
title_full Neuroanatomical abnormalities in a nonhuman primate model of congenital Zika virus infection
title_fullStr Neuroanatomical abnormalities in a nonhuman primate model of congenital Zika virus infection
title_full_unstemmed Neuroanatomical abnormalities in a nonhuman primate model of congenital Zika virus infection
title_short Neuroanatomical abnormalities in a nonhuman primate model of congenital Zika virus infection
title_sort neuroanatomical abnormalities in a nonhuman primate model of congenital zika virus infection
topic Microbiology and Infectious Disease
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8906804/
https://www.ncbi.nlm.nih.gov/pubmed/35261339
http://dx.doi.org/10.7554/eLife.64734
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