Cargando…

Anti-Dlx5 Retards the Progression of Osteoarthritis through Inhibiting Chondrocyte Hypertrophy and Apoptosis

Osteoarthritis is a common degenerative joint disease that can cause pain and disability in patients. There is still a lack of effective treatments to improve pathological changes of osteoarthritis cartilages and reverse the progression of osteoarthritis. Our study aimed to investigate the role of D...

Descripción completa

Detalles Bibliográficos
Autores principales: Lu, Ye, Zhang, Chengyuan, Jiang, Shilin, Yuan, Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8906946/
https://www.ncbi.nlm.nih.gov/pubmed/35280506
http://dx.doi.org/10.1155/2022/5019920
_version_ 1784665527041392640
author Lu, Ye
Zhang, Chengyuan
Jiang, Shilin
Yuan, Feng
author_facet Lu, Ye
Zhang, Chengyuan
Jiang, Shilin
Yuan, Feng
author_sort Lu, Ye
collection PubMed
description Osteoarthritis is a common degenerative joint disease that can cause pain and disability in patients. There is still a lack of effective treatments to improve pathological changes of osteoarthritis cartilages and reverse the progression of osteoarthritis. Our study aimed to investigate the role of Dlx5 in papain-induced osteoarthritis. Osteoarthritis was induced through intraarticular injection of papain. The pathological damage of cartilage tissues was analyzed by H&E staining. The apoptosis of cartilage tissues was detected by TUNEL assay. Immunohistochemical staining was performed to detect DLX5 and BMP-2. Western blot was performed to detect the expressions of SP7, caspase-3, and MYC. The results showed that administration of anti-Dlx5 improved pathological changes of osteoarthritis cartilages, characterized by decreased chondrocyte proliferation, chondrocyte hypertrophy, and matrix damage. Anti-Dlx5 treatment decreased the expressions of BMP-2 and SP7, which are positive regulators of chondrocyte hypertrophy. Moreover, MYC and caspase-3, the critical mediators for chondrocyte apoptosis, were both decreased after anti-Dlx5 treatment. In conclusion, anti-Dlx5 retarded the progression of osteoarthritis by downregulating chondrocyte hypertrophy and chondrocyte apoptosis-related genes. Our findings suggests that Dlx5 is a promising target for osteoarthritis treatment.
format Online
Article
Text
id pubmed-8906946
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-89069462022-03-10 Anti-Dlx5 Retards the Progression of Osteoarthritis through Inhibiting Chondrocyte Hypertrophy and Apoptosis Lu, Ye Zhang, Chengyuan Jiang, Shilin Yuan, Feng Evid Based Complement Alternat Med Research Article Osteoarthritis is a common degenerative joint disease that can cause pain and disability in patients. There is still a lack of effective treatments to improve pathological changes of osteoarthritis cartilages and reverse the progression of osteoarthritis. Our study aimed to investigate the role of Dlx5 in papain-induced osteoarthritis. Osteoarthritis was induced through intraarticular injection of papain. The pathological damage of cartilage tissues was analyzed by H&E staining. The apoptosis of cartilage tissues was detected by TUNEL assay. Immunohistochemical staining was performed to detect DLX5 and BMP-2. Western blot was performed to detect the expressions of SP7, caspase-3, and MYC. The results showed that administration of anti-Dlx5 improved pathological changes of osteoarthritis cartilages, characterized by decreased chondrocyte proliferation, chondrocyte hypertrophy, and matrix damage. Anti-Dlx5 treatment decreased the expressions of BMP-2 and SP7, which are positive regulators of chondrocyte hypertrophy. Moreover, MYC and caspase-3, the critical mediators for chondrocyte apoptosis, were both decreased after anti-Dlx5 treatment. In conclusion, anti-Dlx5 retarded the progression of osteoarthritis by downregulating chondrocyte hypertrophy and chondrocyte apoptosis-related genes. Our findings suggests that Dlx5 is a promising target for osteoarthritis treatment. Hindawi 2022-03-02 /pmc/articles/PMC8906946/ /pubmed/35280506 http://dx.doi.org/10.1155/2022/5019920 Text en Copyright © 2022 Ye Lu et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Lu, Ye
Zhang, Chengyuan
Jiang, Shilin
Yuan, Feng
Anti-Dlx5 Retards the Progression of Osteoarthritis through Inhibiting Chondrocyte Hypertrophy and Apoptosis
title Anti-Dlx5 Retards the Progression of Osteoarthritis through Inhibiting Chondrocyte Hypertrophy and Apoptosis
title_full Anti-Dlx5 Retards the Progression of Osteoarthritis through Inhibiting Chondrocyte Hypertrophy and Apoptosis
title_fullStr Anti-Dlx5 Retards the Progression of Osteoarthritis through Inhibiting Chondrocyte Hypertrophy and Apoptosis
title_full_unstemmed Anti-Dlx5 Retards the Progression of Osteoarthritis through Inhibiting Chondrocyte Hypertrophy and Apoptosis
title_short Anti-Dlx5 Retards the Progression of Osteoarthritis through Inhibiting Chondrocyte Hypertrophy and Apoptosis
title_sort anti-dlx5 retards the progression of osteoarthritis through inhibiting chondrocyte hypertrophy and apoptosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8906946/
https://www.ncbi.nlm.nih.gov/pubmed/35280506
http://dx.doi.org/10.1155/2022/5019920
work_keys_str_mv AT luye antidlx5retardstheprogressionofosteoarthritisthroughinhibitingchondrocytehypertrophyandapoptosis
AT zhangchengyuan antidlx5retardstheprogressionofosteoarthritisthroughinhibitingchondrocytehypertrophyandapoptosis
AT jiangshilin antidlx5retardstheprogressionofosteoarthritisthroughinhibitingchondrocytehypertrophyandapoptosis
AT yuanfeng antidlx5retardstheprogressionofosteoarthritisthroughinhibitingchondrocytehypertrophyandapoptosis