Not just a background: pH buffers do interact with lanthanide ions—a Europium(III) case study

The interaction between Eu(III) ion and different pH buffers, popular in biology and biochemistry, viz. HEPES, PIPES, MES, MOPS, and TRIS, has been studied by solution nuclear magnetic resonance spectroscopy (NMR) and time-resolved laser-induced fluorescence spectroscopy (TRLFS) techniques. The Good...

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Detalles Bibliográficos
Autores principales: Mandal, Poulami, Kretzschmar, Jerome, Drobot, Björn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2022
Materias:
Original Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8907096/
https://www.ncbi.nlm.nih.gov/pubmed/35150337
http://dx.doi.org/10.1007/s00775-022-01930-x
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author Mandal, Poulami
Kretzschmar, Jerome
Drobot, Björn
author_facet Mandal, Poulami
Kretzschmar, Jerome
Drobot, Björn
author_sort Mandal, Poulami
collection PubMed
description The interaction between Eu(III) ion and different pH buffers, popular in biology and biochemistry, viz. HEPES, PIPES, MES, MOPS, and TRIS, has been studied by solution nuclear magnetic resonance spectroscopy (NMR) and time-resolved laser-induced fluorescence spectroscopy (TRLFS) techniques. The Good’s buffers reveal non-negligible interaction with Eu(III) as determined from their complex stability constants, where the sites of interaction are the morpholine and piperazine nitrogen atoms, respectively. In contrast, TRIS buffer shows practically no affinity towards Eu(III). Therefore, when investigating lanthanides, TRIS buffer should be preferred over Good’s buffers. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00775-022-01930-x.
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spelling pubmed-89070962022-03-15 Not just a background: pH buffers do interact with lanthanide ions—a Europium(III) case study Mandal, Poulami Kretzschmar, Jerome Drobot, Björn J Biol Inorg Chem Original Paper The interaction between Eu(III) ion and different pH buffers, popular in biology and biochemistry, viz. HEPES, PIPES, MES, MOPS, and TRIS, has been studied by solution nuclear magnetic resonance spectroscopy (NMR) and time-resolved laser-induced fluorescence spectroscopy (TRLFS) techniques. The Good’s buffers reveal non-negligible interaction with Eu(III) as determined from their complex stability constants, where the sites of interaction are the morpholine and piperazine nitrogen atoms, respectively. In contrast, TRIS buffer shows practically no affinity towards Eu(III). Therefore, when investigating lanthanides, TRIS buffer should be preferred over Good’s buffers. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00775-022-01930-x. Springer International Publishing 2022-02-12 2022 /pmc/articles/PMC8907096/ /pubmed/35150337 http://dx.doi.org/10.1007/s00775-022-01930-x Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Paper
Mandal, Poulami
Kretzschmar, Jerome
Drobot, Björn
Not just a background: pH buffers do interact with lanthanide ions—a Europium(III) case study
title Not just a background: pH buffers do interact with lanthanide ions—a Europium(III) case study
title_full Not just a background: pH buffers do interact with lanthanide ions—a Europium(III) case study
title_fullStr Not just a background: pH buffers do interact with lanthanide ions—a Europium(III) case study
title_full_unstemmed Not just a background: pH buffers do interact with lanthanide ions—a Europium(III) case study
title_short Not just a background: pH buffers do interact with lanthanide ions—a Europium(III) case study
title_sort not just a background: ph buffers do interact with lanthanide ions—a europium(iii) case study
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8907096/
https://www.ncbi.nlm.nih.gov/pubmed/35150337
http://dx.doi.org/10.1007/s00775-022-01930-x
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