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Characterization and Comparison of the Rumen Luminal and Epithelial Microbiome Profiles Using Metagenomic Sequencing Technique

Bacterial dysbiosis as a result of nutritional, bacterial, viral, and parasitic gastrointestinal infections can adversely affect the metabolism, productivity, and overall health of cattle. The purpose of this project was to characterize the commensal microbiota present in two locations of the rumen...

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Autores principales: Stockler, Ricardo M., Hallowell, Haley, Higgins, Keah V., Groover, Erin S., Hiltbold, Elizabeth M., Newcomer, Benjamin, Walz, Paul H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8907629/
https://www.ncbi.nlm.nih.gov/pubmed/35280141
http://dx.doi.org/10.3389/fvets.2022.799063
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author Stockler, Ricardo M.
Hallowell, Haley
Higgins, Keah V.
Groover, Erin S.
Hiltbold, Elizabeth M.
Newcomer, Benjamin
Walz, Paul H.
author_facet Stockler, Ricardo M.
Hallowell, Haley
Higgins, Keah V.
Groover, Erin S.
Hiltbold, Elizabeth M.
Newcomer, Benjamin
Walz, Paul H.
author_sort Stockler, Ricardo M.
collection PubMed
description Bacterial dysbiosis as a result of nutritional, bacterial, viral, and parasitic gastrointestinal infections can adversely affect the metabolism, productivity, and overall health of cattle. The purpose of this project was to characterize the commensal microbiota present in two locations of the rumen concomitantly in vivo with the animals undergoing habitual husbandry, as it was hypothesized that there are major differences in the commensal microbiota present in the two locations of the adult bovine major forestomach. A surgically fitted rumen cannula was used to allow ruminal lumen contents and mucosal biopsies to be collected from six crossbred yearling steers. In order to assess as much environmental and individual steer microbiota variation as possible, each animal was randomly sampled three times over a 3 week period. 16S rRNA sequencing was performed to provide a detailed descriptive analysis from phylum to genus taxonomic level. Significant differences were observed between luminal and epimural bacterial populations in the bovine rumen. As expected, a core microbiome composed by Firmicutes and Bacteroidetes represented over 90% of the microbiome, however, further analysis showed distinct diversity and distribution of the microbiome between the two locations. Characterizing the gastrointestinal microbiome in vivo is imperative. The novelty and the contribution of this study to the literature is the use of live cattle which allowed real-time sample collections and analysis of the rumen microbiome providing an understanding of what is normal in the live animal.
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spelling pubmed-89076292022-03-11 Characterization and Comparison of the Rumen Luminal and Epithelial Microbiome Profiles Using Metagenomic Sequencing Technique Stockler, Ricardo M. Hallowell, Haley Higgins, Keah V. Groover, Erin S. Hiltbold, Elizabeth M. Newcomer, Benjamin Walz, Paul H. Front Vet Sci Veterinary Science Bacterial dysbiosis as a result of nutritional, bacterial, viral, and parasitic gastrointestinal infections can adversely affect the metabolism, productivity, and overall health of cattle. The purpose of this project was to characterize the commensal microbiota present in two locations of the rumen concomitantly in vivo with the animals undergoing habitual husbandry, as it was hypothesized that there are major differences in the commensal microbiota present in the two locations of the adult bovine major forestomach. A surgically fitted rumen cannula was used to allow ruminal lumen contents and mucosal biopsies to be collected from six crossbred yearling steers. In order to assess as much environmental and individual steer microbiota variation as possible, each animal was randomly sampled three times over a 3 week period. 16S rRNA sequencing was performed to provide a detailed descriptive analysis from phylum to genus taxonomic level. Significant differences were observed between luminal and epimural bacterial populations in the bovine rumen. As expected, a core microbiome composed by Firmicutes and Bacteroidetes represented over 90% of the microbiome, however, further analysis showed distinct diversity and distribution of the microbiome between the two locations. Characterizing the gastrointestinal microbiome in vivo is imperative. The novelty and the contribution of this study to the literature is the use of live cattle which allowed real-time sample collections and analysis of the rumen microbiome providing an understanding of what is normal in the live animal. Frontiers Media S.A. 2022-02-24 /pmc/articles/PMC8907629/ /pubmed/35280141 http://dx.doi.org/10.3389/fvets.2022.799063 Text en Copyright © 2022 Stockler, Hallowell, Higgins, Groover, Hiltbold, Newcomer and Walz. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Stockler, Ricardo M.
Hallowell, Haley
Higgins, Keah V.
Groover, Erin S.
Hiltbold, Elizabeth M.
Newcomer, Benjamin
Walz, Paul H.
Characterization and Comparison of the Rumen Luminal and Epithelial Microbiome Profiles Using Metagenomic Sequencing Technique
title Characterization and Comparison of the Rumen Luminal and Epithelial Microbiome Profiles Using Metagenomic Sequencing Technique
title_full Characterization and Comparison of the Rumen Luminal and Epithelial Microbiome Profiles Using Metagenomic Sequencing Technique
title_fullStr Characterization and Comparison of the Rumen Luminal and Epithelial Microbiome Profiles Using Metagenomic Sequencing Technique
title_full_unstemmed Characterization and Comparison of the Rumen Luminal and Epithelial Microbiome Profiles Using Metagenomic Sequencing Technique
title_short Characterization and Comparison of the Rumen Luminal and Epithelial Microbiome Profiles Using Metagenomic Sequencing Technique
title_sort characterization and comparison of the rumen luminal and epithelial microbiome profiles using metagenomic sequencing technique
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8907629/
https://www.ncbi.nlm.nih.gov/pubmed/35280141
http://dx.doi.org/10.3389/fvets.2022.799063
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