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ROOT HAIR DEFECTIVE3 Is a Receptor for Selective Autophagy of the Endoplasmic Reticulum in Arabidopsis

ROOT HAIR DEFECTIVE3 (RHD3) is a plant member of atlastin GTPases, which belong to an evolutionally conserved family of proteins that mediate the homotypic fusion of the endoplasmic reticulum (ER). An atlastin in mammalian cells has recently been shown to act as an ER-phagy receptor for selective au...

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Autores principales: Sun, Jiaqi, Wang, Weina, Zheng, Huanquan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8907713/
https://www.ncbi.nlm.nih.gov/pubmed/35283874
http://dx.doi.org/10.3389/fpls.2022.817251
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author Sun, Jiaqi
Wang, Weina
Zheng, Huanquan
author_facet Sun, Jiaqi
Wang, Weina
Zheng, Huanquan
author_sort Sun, Jiaqi
collection PubMed
description ROOT HAIR DEFECTIVE3 (RHD3) is a plant member of atlastin GTPases, which belong to an evolutionally conserved family of proteins that mediate the homotypic fusion of the endoplasmic reticulum (ER). An atlastin in mammalian cells has recently been shown to act as an ER-phagy receptor for selective autophagy of the ER (ER-phagy) during nutrient starvation. Although RHD3 has been indicated to play a role in ER stress response, it is not very clear how RHD3 is involved in the process. In this study, we showed that the rhd3 mutant is hyposensitive to ER as well as salt stress. We employed an YFP-tagged ER membrane marker YFP-TMC to monitor the efficiency of ER-phagy microscopically and biochemically. We found that rhd3 is defective in ER-phagy under ER stress. Furthermore, there is an increased association of YFP-RHD3 with ATG8e-marked autophagosomes. YFP-RHD3 is also visible with ATG8e in the vacuole, and there is a breakdown of YFP-RHD3 under ER stress. RHD3 has two putative ATG8 interaction motifs (AIM1-2). We revealed that RHD3 but not RHD3(ΔAIM1) physically interacts with ATG8, a core autophagosomal component that interacts with various receptor proteins to recruit cargos for degradation by selective autophagy. Furthermore, their interaction is enhanced under ER stress. We thus propose that RHD3 acts as an ER-phagy receptor under ER stress to promote ER-phagy in Arabidopsis.
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spelling pubmed-89077132022-03-11 ROOT HAIR DEFECTIVE3 Is a Receptor for Selective Autophagy of the Endoplasmic Reticulum in Arabidopsis Sun, Jiaqi Wang, Weina Zheng, Huanquan Front Plant Sci Plant Science ROOT HAIR DEFECTIVE3 (RHD3) is a plant member of atlastin GTPases, which belong to an evolutionally conserved family of proteins that mediate the homotypic fusion of the endoplasmic reticulum (ER). An atlastin in mammalian cells has recently been shown to act as an ER-phagy receptor for selective autophagy of the ER (ER-phagy) during nutrient starvation. Although RHD3 has been indicated to play a role in ER stress response, it is not very clear how RHD3 is involved in the process. In this study, we showed that the rhd3 mutant is hyposensitive to ER as well as salt stress. We employed an YFP-tagged ER membrane marker YFP-TMC to monitor the efficiency of ER-phagy microscopically and biochemically. We found that rhd3 is defective in ER-phagy under ER stress. Furthermore, there is an increased association of YFP-RHD3 with ATG8e-marked autophagosomes. YFP-RHD3 is also visible with ATG8e in the vacuole, and there is a breakdown of YFP-RHD3 under ER stress. RHD3 has two putative ATG8 interaction motifs (AIM1-2). We revealed that RHD3 but not RHD3(ΔAIM1) physically interacts with ATG8, a core autophagosomal component that interacts with various receptor proteins to recruit cargos for degradation by selective autophagy. Furthermore, their interaction is enhanced under ER stress. We thus propose that RHD3 acts as an ER-phagy receptor under ER stress to promote ER-phagy in Arabidopsis. Frontiers Media S.A. 2022-02-24 /pmc/articles/PMC8907713/ /pubmed/35283874 http://dx.doi.org/10.3389/fpls.2022.817251 Text en Copyright © 2022 Sun, Wang and Zheng. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Sun, Jiaqi
Wang, Weina
Zheng, Huanquan
ROOT HAIR DEFECTIVE3 Is a Receptor for Selective Autophagy of the Endoplasmic Reticulum in Arabidopsis
title ROOT HAIR DEFECTIVE3 Is a Receptor for Selective Autophagy of the Endoplasmic Reticulum in Arabidopsis
title_full ROOT HAIR DEFECTIVE3 Is a Receptor for Selective Autophagy of the Endoplasmic Reticulum in Arabidopsis
title_fullStr ROOT HAIR DEFECTIVE3 Is a Receptor for Selective Autophagy of the Endoplasmic Reticulum in Arabidopsis
title_full_unstemmed ROOT HAIR DEFECTIVE3 Is a Receptor for Selective Autophagy of the Endoplasmic Reticulum in Arabidopsis
title_short ROOT HAIR DEFECTIVE3 Is a Receptor for Selective Autophagy of the Endoplasmic Reticulum in Arabidopsis
title_sort root hair defective3 is a receptor for selective autophagy of the endoplasmic reticulum in arabidopsis
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8907713/
https://www.ncbi.nlm.nih.gov/pubmed/35283874
http://dx.doi.org/10.3389/fpls.2022.817251
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AT zhenghuanquan roothairdefective3isareceptorforselectiveautophagyoftheendoplasmicreticuluminarabidopsis