Cargando…

Immunomodulatory activity of aqueous extract from Crassostrea sikamea in the splenocytes of Sprague‐Dawley rats

Crassostrea sikamea (C. sikamea) is used as an important edible and medicinal seafood in China. In the present study, an aqueous extract of C. sikamea (AECs) was prepared, and its immunomodulatory effects on rat splenocytes were studied. 3‐(4,5‐dimethylthiazol‐2‐yl)‐5‐(3‐carboxymethoxyphenyl)‐2‐(4‐s...

Descripción completa

Detalles Bibliográficos
Autores principales: Guo, Guannan, Kong, Ying, Su, Jie, Wang, Geng, Zhang, Muqing, Wang, Shuyue, Song, Zhenbo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8907723/
https://www.ncbi.nlm.nih.gov/pubmed/35282010
http://dx.doi.org/10.1002/fsn3.2710
_version_ 1784665714339086336
author Guo, Guannan
Kong, Ying
Su, Jie
Wang, Geng
Zhang, Muqing
Wang, Shuyue
Song, Zhenbo
author_facet Guo, Guannan
Kong, Ying
Su, Jie
Wang, Geng
Zhang, Muqing
Wang, Shuyue
Song, Zhenbo
author_sort Guo, Guannan
collection PubMed
description Crassostrea sikamea (C. sikamea) is used as an important edible and medicinal seafood in China. In the present study, an aqueous extract of C. sikamea (AECs) was prepared, and its immunomodulatory effects on rat splenocytes were studied. 3‐(4,5‐dimethylthiazol‐2‐yl)‐5‐(3‐carboxymethoxyphenyl)‐2‐(4‐sulfophenyl)‐2H‐tetrazolium (MTS) assay revealed that AECs was able to promote splenocyte proliferation. Moreover, flow cytometry revealed that AECs treatment markedly altered the populations of splenic lymphocyte subtypes. Data from real‐time quantitative PCR (RT‐qPCR) and enzyme‐linked immunosorbent assay (ELISA) showed that AECs promoted the mRNA expression and secretion of TNF‐α, IL‐2, IL‐6, IL‐12, and IFN‐γ. Mechanistically, p38 MAPK phosphorylation in splenocytes was significantly upregulated under AECs treatment and p38 MAPK inhibitor reversed the promoting effect of AECs on the expression of inflammatory cytokines. Collectively, our novel evidence suggests that AECs exhibits immunomodulatory activity in vitro, supporting the further application of C. sikamea as a potential functional food.
format Online
Article
Text
id pubmed-8907723
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-89077232022-03-10 Immunomodulatory activity of aqueous extract from Crassostrea sikamea in the splenocytes of Sprague‐Dawley rats Guo, Guannan Kong, Ying Su, Jie Wang, Geng Zhang, Muqing Wang, Shuyue Song, Zhenbo Food Sci Nutr Original Articles Crassostrea sikamea (C. sikamea) is used as an important edible and medicinal seafood in China. In the present study, an aqueous extract of C. sikamea (AECs) was prepared, and its immunomodulatory effects on rat splenocytes were studied. 3‐(4,5‐dimethylthiazol‐2‐yl)‐5‐(3‐carboxymethoxyphenyl)‐2‐(4‐sulfophenyl)‐2H‐tetrazolium (MTS) assay revealed that AECs was able to promote splenocyte proliferation. Moreover, flow cytometry revealed that AECs treatment markedly altered the populations of splenic lymphocyte subtypes. Data from real‐time quantitative PCR (RT‐qPCR) and enzyme‐linked immunosorbent assay (ELISA) showed that AECs promoted the mRNA expression and secretion of TNF‐α, IL‐2, IL‐6, IL‐12, and IFN‐γ. Mechanistically, p38 MAPK phosphorylation in splenocytes was significantly upregulated under AECs treatment and p38 MAPK inhibitor reversed the promoting effect of AECs on the expression of inflammatory cytokines. Collectively, our novel evidence suggests that AECs exhibits immunomodulatory activity in vitro, supporting the further application of C. sikamea as a potential functional food. John Wiley and Sons Inc. 2022-01-05 /pmc/articles/PMC8907723/ /pubmed/35282010 http://dx.doi.org/10.1002/fsn3.2710 Text en © 2022 The Authors. Food Science & Nutrition published by Wiley Periodicals LLC. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Guo, Guannan
Kong, Ying
Su, Jie
Wang, Geng
Zhang, Muqing
Wang, Shuyue
Song, Zhenbo
Immunomodulatory activity of aqueous extract from Crassostrea sikamea in the splenocytes of Sprague‐Dawley rats
title Immunomodulatory activity of aqueous extract from Crassostrea sikamea in the splenocytes of Sprague‐Dawley rats
title_full Immunomodulatory activity of aqueous extract from Crassostrea sikamea in the splenocytes of Sprague‐Dawley rats
title_fullStr Immunomodulatory activity of aqueous extract from Crassostrea sikamea in the splenocytes of Sprague‐Dawley rats
title_full_unstemmed Immunomodulatory activity of aqueous extract from Crassostrea sikamea in the splenocytes of Sprague‐Dawley rats
title_short Immunomodulatory activity of aqueous extract from Crassostrea sikamea in the splenocytes of Sprague‐Dawley rats
title_sort immunomodulatory activity of aqueous extract from crassostrea sikamea in the splenocytes of sprague‐dawley rats
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8907723/
https://www.ncbi.nlm.nih.gov/pubmed/35282010
http://dx.doi.org/10.1002/fsn3.2710
work_keys_str_mv AT guoguannan immunomodulatoryactivityofaqueousextractfromcrassostreasikameainthesplenocytesofspraguedawleyrats
AT kongying immunomodulatoryactivityofaqueousextractfromcrassostreasikameainthesplenocytesofspraguedawleyrats
AT sujie immunomodulatoryactivityofaqueousextractfromcrassostreasikameainthesplenocytesofspraguedawleyrats
AT wanggeng immunomodulatoryactivityofaqueousextractfromcrassostreasikameainthesplenocytesofspraguedawleyrats
AT zhangmuqing immunomodulatoryactivityofaqueousextractfromcrassostreasikameainthesplenocytesofspraguedawleyrats
AT wangshuyue immunomodulatoryactivityofaqueousextractfromcrassostreasikameainthesplenocytesofspraguedawleyrats
AT songzhenbo immunomodulatoryactivityofaqueousextractfromcrassostreasikameainthesplenocytesofspraguedawleyrats