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Immunomodulatory activity of aqueous extract from Crassostrea sikamea in the splenocytes of Sprague‐Dawley rats
Crassostrea sikamea (C. sikamea) is used as an important edible and medicinal seafood in China. In the present study, an aqueous extract of C. sikamea (AECs) was prepared, and its immunomodulatory effects on rat splenocytes were studied. 3‐(4,5‐dimethylthiazol‐2‐yl)‐5‐(3‐carboxymethoxyphenyl)‐2‐(4‐s...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8907723/ https://www.ncbi.nlm.nih.gov/pubmed/35282010 http://dx.doi.org/10.1002/fsn3.2710 |
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author | Guo, Guannan Kong, Ying Su, Jie Wang, Geng Zhang, Muqing Wang, Shuyue Song, Zhenbo |
author_facet | Guo, Guannan Kong, Ying Su, Jie Wang, Geng Zhang, Muqing Wang, Shuyue Song, Zhenbo |
author_sort | Guo, Guannan |
collection | PubMed |
description | Crassostrea sikamea (C. sikamea) is used as an important edible and medicinal seafood in China. In the present study, an aqueous extract of C. sikamea (AECs) was prepared, and its immunomodulatory effects on rat splenocytes were studied. 3‐(4,5‐dimethylthiazol‐2‐yl)‐5‐(3‐carboxymethoxyphenyl)‐2‐(4‐sulfophenyl)‐2H‐tetrazolium (MTS) assay revealed that AECs was able to promote splenocyte proliferation. Moreover, flow cytometry revealed that AECs treatment markedly altered the populations of splenic lymphocyte subtypes. Data from real‐time quantitative PCR (RT‐qPCR) and enzyme‐linked immunosorbent assay (ELISA) showed that AECs promoted the mRNA expression and secretion of TNF‐α, IL‐2, IL‐6, IL‐12, and IFN‐γ. Mechanistically, p38 MAPK phosphorylation in splenocytes was significantly upregulated under AECs treatment and p38 MAPK inhibitor reversed the promoting effect of AECs on the expression of inflammatory cytokines. Collectively, our novel evidence suggests that AECs exhibits immunomodulatory activity in vitro, supporting the further application of C. sikamea as a potential functional food. |
format | Online Article Text |
id | pubmed-8907723 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-89077232022-03-10 Immunomodulatory activity of aqueous extract from Crassostrea sikamea in the splenocytes of Sprague‐Dawley rats Guo, Guannan Kong, Ying Su, Jie Wang, Geng Zhang, Muqing Wang, Shuyue Song, Zhenbo Food Sci Nutr Original Articles Crassostrea sikamea (C. sikamea) is used as an important edible and medicinal seafood in China. In the present study, an aqueous extract of C. sikamea (AECs) was prepared, and its immunomodulatory effects on rat splenocytes were studied. 3‐(4,5‐dimethylthiazol‐2‐yl)‐5‐(3‐carboxymethoxyphenyl)‐2‐(4‐sulfophenyl)‐2H‐tetrazolium (MTS) assay revealed that AECs was able to promote splenocyte proliferation. Moreover, flow cytometry revealed that AECs treatment markedly altered the populations of splenic lymphocyte subtypes. Data from real‐time quantitative PCR (RT‐qPCR) and enzyme‐linked immunosorbent assay (ELISA) showed that AECs promoted the mRNA expression and secretion of TNF‐α, IL‐2, IL‐6, IL‐12, and IFN‐γ. Mechanistically, p38 MAPK phosphorylation in splenocytes was significantly upregulated under AECs treatment and p38 MAPK inhibitor reversed the promoting effect of AECs on the expression of inflammatory cytokines. Collectively, our novel evidence suggests that AECs exhibits immunomodulatory activity in vitro, supporting the further application of C. sikamea as a potential functional food. John Wiley and Sons Inc. 2022-01-05 /pmc/articles/PMC8907723/ /pubmed/35282010 http://dx.doi.org/10.1002/fsn3.2710 Text en © 2022 The Authors. Food Science & Nutrition published by Wiley Periodicals LLC. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Guo, Guannan Kong, Ying Su, Jie Wang, Geng Zhang, Muqing Wang, Shuyue Song, Zhenbo Immunomodulatory activity of aqueous extract from Crassostrea sikamea in the splenocytes of Sprague‐Dawley rats |
title | Immunomodulatory activity of aqueous extract from Crassostrea sikamea in the splenocytes of Sprague‐Dawley rats |
title_full | Immunomodulatory activity of aqueous extract from Crassostrea sikamea in the splenocytes of Sprague‐Dawley rats |
title_fullStr | Immunomodulatory activity of aqueous extract from Crassostrea sikamea in the splenocytes of Sprague‐Dawley rats |
title_full_unstemmed | Immunomodulatory activity of aqueous extract from Crassostrea sikamea in the splenocytes of Sprague‐Dawley rats |
title_short | Immunomodulatory activity of aqueous extract from Crassostrea sikamea in the splenocytes of Sprague‐Dawley rats |
title_sort | immunomodulatory activity of aqueous extract from crassostrea sikamea in the splenocytes of sprague‐dawley rats |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8907723/ https://www.ncbi.nlm.nih.gov/pubmed/35282010 http://dx.doi.org/10.1002/fsn3.2710 |
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