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Conserved Regulatory Pathways for Stock-Scion Healing Revealed by Comparative Analysis of Arabidopsis and Tomato Grafting Transcriptomes

Many plants can successfully join root and shoot sections at cut surfaces when severed at the stem. Graft healing is complex and conserved in diverse taxonomic groups with different vascular structures. Herein, we compared transcriptome data from autografted and separated stem sections of Arabidopsi...

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Autores principales: Xie, Lulu, Tian, Jianfan, Peng, Lixin, Cui, Qingqing, Liu, Yang, Liu, Jiyang, Li, Fu, Zhang, Siyuan, Gao, Jianchang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8908109/
https://www.ncbi.nlm.nih.gov/pubmed/35281699
http://dx.doi.org/10.3389/fpls.2021.810465
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author Xie, Lulu
Tian, Jianfan
Peng, Lixin
Cui, Qingqing
Liu, Yang
Liu, Jiyang
Li, Fu
Zhang, Siyuan
Gao, Jianchang
author_facet Xie, Lulu
Tian, Jianfan
Peng, Lixin
Cui, Qingqing
Liu, Yang
Liu, Jiyang
Li, Fu
Zhang, Siyuan
Gao, Jianchang
author_sort Xie, Lulu
collection PubMed
description Many plants can successfully join root and shoot sections at cut surfaces when severed at the stem. Graft healing is complex and conserved in diverse taxonomic groups with different vascular structures. Herein, we compared transcriptome data from autografted and separated stem sections of Arabidopsis thaliana and tomato (Solanum lycopersicum) to explore changes related to graft healing. Using orthologous gene pairs identified between the two species, temperal expression patterns of evolutionary associated genes in grafted top and bottom, separated top and bottom, and intact stems were exhibited. Genes with expression preference indicate functional diversification of genes related to anatomical structure and cellular development in the two species. Expression profiles of the variable genes revealed common pathways operating during graft healing, including phenylpropanoid metabolism, response to oxygen-containing compounds, xylan, and cell wall biogenesis, mitosis and the cell cycle, carboxylic acid catabolism, and meristem structural organization. In addition, vascular differentiation related NAC domain transcription factors and genome-wide members in Arabidopsis and tomato were used for phylogenetic and expression analysis. Expression differences were largely consistent with sequence differences, reflecting high similarity for protein-coding and regulatory regions of individual clades. NAC proteins mainly clustered in accordance with their reported functions in xylem differentiation or cambium formation. The putative conserved mechanisms suggested by conserved genes and functions could help to expand graft healing theory to a wider range of species, and temporal fluctuations in common pathways imply conserved biological processes during graft healing.
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spelling pubmed-89081092022-03-11 Conserved Regulatory Pathways for Stock-Scion Healing Revealed by Comparative Analysis of Arabidopsis and Tomato Grafting Transcriptomes Xie, Lulu Tian, Jianfan Peng, Lixin Cui, Qingqing Liu, Yang Liu, Jiyang Li, Fu Zhang, Siyuan Gao, Jianchang Front Plant Sci Plant Science Many plants can successfully join root and shoot sections at cut surfaces when severed at the stem. Graft healing is complex and conserved in diverse taxonomic groups with different vascular structures. Herein, we compared transcriptome data from autografted and separated stem sections of Arabidopsis thaliana and tomato (Solanum lycopersicum) to explore changes related to graft healing. Using orthologous gene pairs identified between the two species, temperal expression patterns of evolutionary associated genes in grafted top and bottom, separated top and bottom, and intact stems were exhibited. Genes with expression preference indicate functional diversification of genes related to anatomical structure and cellular development in the two species. Expression profiles of the variable genes revealed common pathways operating during graft healing, including phenylpropanoid metabolism, response to oxygen-containing compounds, xylan, and cell wall biogenesis, mitosis and the cell cycle, carboxylic acid catabolism, and meristem structural organization. In addition, vascular differentiation related NAC domain transcription factors and genome-wide members in Arabidopsis and tomato were used for phylogenetic and expression analysis. Expression differences were largely consistent with sequence differences, reflecting high similarity for protein-coding and regulatory regions of individual clades. NAC proteins mainly clustered in accordance with their reported functions in xylem differentiation or cambium formation. The putative conserved mechanisms suggested by conserved genes and functions could help to expand graft healing theory to a wider range of species, and temporal fluctuations in common pathways imply conserved biological processes during graft healing. Frontiers Media S.A. 2022-02-24 /pmc/articles/PMC8908109/ /pubmed/35281699 http://dx.doi.org/10.3389/fpls.2021.810465 Text en Copyright © 2022 Xie, Tian, Peng, Cui, Liu, Liu, Li, Zhang and Gao. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Xie, Lulu
Tian, Jianfan
Peng, Lixin
Cui, Qingqing
Liu, Yang
Liu, Jiyang
Li, Fu
Zhang, Siyuan
Gao, Jianchang
Conserved Regulatory Pathways for Stock-Scion Healing Revealed by Comparative Analysis of Arabidopsis and Tomato Grafting Transcriptomes
title Conserved Regulatory Pathways for Stock-Scion Healing Revealed by Comparative Analysis of Arabidopsis and Tomato Grafting Transcriptomes
title_full Conserved Regulatory Pathways for Stock-Scion Healing Revealed by Comparative Analysis of Arabidopsis and Tomato Grafting Transcriptomes
title_fullStr Conserved Regulatory Pathways for Stock-Scion Healing Revealed by Comparative Analysis of Arabidopsis and Tomato Grafting Transcriptomes
title_full_unstemmed Conserved Regulatory Pathways for Stock-Scion Healing Revealed by Comparative Analysis of Arabidopsis and Tomato Grafting Transcriptomes
title_short Conserved Regulatory Pathways for Stock-Scion Healing Revealed by Comparative Analysis of Arabidopsis and Tomato Grafting Transcriptomes
title_sort conserved regulatory pathways for stock-scion healing revealed by comparative analysis of arabidopsis and tomato grafting transcriptomes
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8908109/
https://www.ncbi.nlm.nih.gov/pubmed/35281699
http://dx.doi.org/10.3389/fpls.2021.810465
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