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An animal experiment study on the application of indocyanine green angiography in the harvest of multi-angiosome perforator flap

BACKGROUND: This study sought to explore the application value of indocyanine green angiography (ICGA) in the harvest of multi-angiosome perforator flap and the effect of low molecular weight heparin (LMWH) on the survival of postoperative flap. METHODS: Twenty-four SD male rats were selected to con...

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Autores principales: Wanyan, Chaojie, Wu, Zhongming, Zhang, Fengrui, Li, Huan, Yang, Zihui, Wang, Jun, Han, Xuejiao, Yang, Xiangming, Lei, Delin, Yang, Xinjie, Wei, Jianhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AME Publishing Company 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8908162/
https://www.ncbi.nlm.nih.gov/pubmed/35280416
http://dx.doi.org/10.21037/atm-22-220
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author Wanyan, Chaojie
Wu, Zhongming
Zhang, Fengrui
Li, Huan
Yang, Zihui
Wang, Jun
Han, Xuejiao
Yang, Xiangming
Lei, Delin
Yang, Xinjie
Wei, Jianhua
author_facet Wanyan, Chaojie
Wu, Zhongming
Zhang, Fengrui
Li, Huan
Yang, Zihui
Wang, Jun
Han, Xuejiao
Yang, Xiangming
Lei, Delin
Yang, Xinjie
Wei, Jianhua
author_sort Wanyan, Chaojie
collection PubMed
description BACKGROUND: This study sought to explore the application value of indocyanine green angiography (ICGA) in the harvest of multi-angiosome perforator flap and the effect of low molecular weight heparin (LMWH) on the survival of postoperative flap. METHODS: Twenty-four SD male rats were selected to construct a three-angiosome perforator flap model with the unilateral iliolumbar artery perforator. They were randomly divided into two groups: the control group was injected with indocyanine green (ICG) into the femoral vein during the operation, and the fluorescence signal was collected and quantitatively analyzed using Real-Time Image Guided System to determine the intraoperative fluorescence imaging length. The experimental group was injected subcutaneously with LMWH (400 U/kg) after 0.5 h postoperatively, and the control group was injected with the same amount of normal saline. The injection was repeated at the same time each day from 0 to 7 days postoperatively. After the flap was sutured in situ, ICGA was performed at 0, 1, 3, 5, and 7 days postoperatively to observe the vascular structure of the two groups of flaps. The flap survival length of the control group was counted at 7 days postoperatively, and the correlation between the intraoperative fluorescence imaging length and the survival length at 7 days postoperatively was calculated. The proportion of distal necrosis of the flaps between the two groups was compared at 7 days postoperatively. RESULTS: The average length of intraoperative fluorescence imaging in the control group was 6.29±0.50 cm, and the survival length of the flap at 7 days postoperatively was 8.24±0.52 cm. The actual survival length was higher than the intraoperative fluorescence imaging length, with a ratio of 1.31±0.08. The difference was statistically significant (P<0.05). At 7 days postoperatively, the flap necrosis ratio of experimental group and control group were 10.92%±1.30% and 19.11%±1.19%, and the flap necrosis ratio of experimental group was lower than that of control group (P<0.001). CONCLUSIONS: ICGA can locate the position of perforator, and can be used to predict and observe the length of distal survival of multi-angiosome perforator flap postoperatively. LMWH can promote the distal survival of flap and reduce flap necrosis.
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spelling pubmed-89081622022-03-11 An animal experiment study on the application of indocyanine green angiography in the harvest of multi-angiosome perforator flap Wanyan, Chaojie Wu, Zhongming Zhang, Fengrui Li, Huan Yang, Zihui Wang, Jun Han, Xuejiao Yang, Xiangming Lei, Delin Yang, Xinjie Wei, Jianhua Ann Transl Med Original Article BACKGROUND: This study sought to explore the application value of indocyanine green angiography (ICGA) in the harvest of multi-angiosome perforator flap and the effect of low molecular weight heparin (LMWH) on the survival of postoperative flap. METHODS: Twenty-four SD male rats were selected to construct a three-angiosome perforator flap model with the unilateral iliolumbar artery perforator. They were randomly divided into two groups: the control group was injected with indocyanine green (ICG) into the femoral vein during the operation, and the fluorescence signal was collected and quantitatively analyzed using Real-Time Image Guided System to determine the intraoperative fluorescence imaging length. The experimental group was injected subcutaneously with LMWH (400 U/kg) after 0.5 h postoperatively, and the control group was injected with the same amount of normal saline. The injection was repeated at the same time each day from 0 to 7 days postoperatively. After the flap was sutured in situ, ICGA was performed at 0, 1, 3, 5, and 7 days postoperatively to observe the vascular structure of the two groups of flaps. The flap survival length of the control group was counted at 7 days postoperatively, and the correlation between the intraoperative fluorescence imaging length and the survival length at 7 days postoperatively was calculated. The proportion of distal necrosis of the flaps between the two groups was compared at 7 days postoperatively. RESULTS: The average length of intraoperative fluorescence imaging in the control group was 6.29±0.50 cm, and the survival length of the flap at 7 days postoperatively was 8.24±0.52 cm. The actual survival length was higher than the intraoperative fluorescence imaging length, with a ratio of 1.31±0.08. The difference was statistically significant (P<0.05). At 7 days postoperatively, the flap necrosis ratio of experimental group and control group were 10.92%±1.30% and 19.11%±1.19%, and the flap necrosis ratio of experimental group was lower than that of control group (P<0.001). CONCLUSIONS: ICGA can locate the position of perforator, and can be used to predict and observe the length of distal survival of multi-angiosome perforator flap postoperatively. LMWH can promote the distal survival of flap and reduce flap necrosis. AME Publishing Company 2022-02 /pmc/articles/PMC8908162/ /pubmed/35280416 http://dx.doi.org/10.21037/atm-22-220 Text en 2022 Annals of Translational Medicine. All rights reserved. https://creativecommons.org/licenses/by-nc-nd/4.0/Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Original Article
Wanyan, Chaojie
Wu, Zhongming
Zhang, Fengrui
Li, Huan
Yang, Zihui
Wang, Jun
Han, Xuejiao
Yang, Xiangming
Lei, Delin
Yang, Xinjie
Wei, Jianhua
An animal experiment study on the application of indocyanine green angiography in the harvest of multi-angiosome perforator flap
title An animal experiment study on the application of indocyanine green angiography in the harvest of multi-angiosome perforator flap
title_full An animal experiment study on the application of indocyanine green angiography in the harvest of multi-angiosome perforator flap
title_fullStr An animal experiment study on the application of indocyanine green angiography in the harvest of multi-angiosome perforator flap
title_full_unstemmed An animal experiment study on the application of indocyanine green angiography in the harvest of multi-angiosome perforator flap
title_short An animal experiment study on the application of indocyanine green angiography in the harvest of multi-angiosome perforator flap
title_sort animal experiment study on the application of indocyanine green angiography in the harvest of multi-angiosome perforator flap
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8908162/
https://www.ncbi.nlm.nih.gov/pubmed/35280416
http://dx.doi.org/10.21037/atm-22-220
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