Using intra-brain drug infusion to investigate neural mechanisms underlying reward-seeking behavior in mice

Brain-region-specific drug infusion is a key way to investigate neural mechanisms underlying behavior and neurological diseases. Here, we present a detailed protocol for cannula implantation, intra-brain drug infusion, and two reward-seeking-related behavioral paradigms in mice: the light/dark box t...

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Detalles Bibliográficos
Autores principales: Xu, Chang, Peng, Beibei, Liu, Shuai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8908249/
https://www.ncbi.nlm.nih.gov/pubmed/35284840
http://dx.doi.org/10.1016/j.xpro.2022.101221
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author Xu, Chang
Peng, Beibei
Liu, Shuai
author_facet Xu, Chang
Peng, Beibei
Liu, Shuai
author_sort Xu, Chang
collection PubMed
description Brain-region-specific drug infusion is a key way to investigate neural mechanisms underlying behavior and neurological diseases. Here, we present a detailed protocol for cannula implantation, intra-brain drug infusion, and two reward-seeking-related behavioral paradigms in mice: the light/dark box test and touchscreen version of progressive ratio test. In addition, we provide a user-friendly Python-based tool for behavioral data analysis. This protocol can be easily adapted to address various research questions related to behavioral pharmacology. For complete details on the use and execution of this protocol, please refer to Peng et al. (2021).
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spelling pubmed-89082492022-03-11 Using intra-brain drug infusion to investigate neural mechanisms underlying reward-seeking behavior in mice Xu, Chang Peng, Beibei Liu, Shuai STAR Protoc Protocol Brain-region-specific drug infusion is a key way to investigate neural mechanisms underlying behavior and neurological diseases. Here, we present a detailed protocol for cannula implantation, intra-brain drug infusion, and two reward-seeking-related behavioral paradigms in mice: the light/dark box test and touchscreen version of progressive ratio test. In addition, we provide a user-friendly Python-based tool for behavioral data analysis. This protocol can be easily adapted to address various research questions related to behavioral pharmacology. For complete details on the use and execution of this protocol, please refer to Peng et al. (2021). Elsevier 2022-03-07 /pmc/articles/PMC8908249/ /pubmed/35284840 http://dx.doi.org/10.1016/j.xpro.2022.101221 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Xu, Chang
Peng, Beibei
Liu, Shuai
Using intra-brain drug infusion to investigate neural mechanisms underlying reward-seeking behavior in mice
title Using intra-brain drug infusion to investigate neural mechanisms underlying reward-seeking behavior in mice
title_full Using intra-brain drug infusion to investigate neural mechanisms underlying reward-seeking behavior in mice
title_fullStr Using intra-brain drug infusion to investigate neural mechanisms underlying reward-seeking behavior in mice
title_full_unstemmed Using intra-brain drug infusion to investigate neural mechanisms underlying reward-seeking behavior in mice
title_short Using intra-brain drug infusion to investigate neural mechanisms underlying reward-seeking behavior in mice
title_sort using intra-brain drug infusion to investigate neural mechanisms underlying reward-seeking behavior in mice
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8908249/
https://www.ncbi.nlm.nih.gov/pubmed/35284840
http://dx.doi.org/10.1016/j.xpro.2022.101221
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