Knock down of target genes by RNA interference in the embryos of lepidopteran insect, Bombyx mori

RNA interference (RNAi) is a technique used for posttranscriptional gene silencing, but lepidopteran insects are not sensitive to RNAi. Here, we present a protocol for knocking down the expression level of target genes by RNAi in Bombyx mori embryos. We describe the preparation of double-stranded RN...

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Detalles Bibliográficos
Autores principales: Xu, Guanfeng, Tian, Yulin, Peng, Yuling, Zheng, Sichun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8908253/
https://www.ncbi.nlm.nih.gov/pubmed/35284831
http://dx.doi.org/10.1016/j.xpro.2022.101219
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author Xu, Guanfeng
Tian, Yulin
Peng, Yuling
Zheng, Sichun
author_facet Xu, Guanfeng
Tian, Yulin
Peng, Yuling
Zheng, Sichun
author_sort Xu, Guanfeng
collection PubMed
description RNA interference (RNAi) is a technique used for posttranscriptional gene silencing, but lepidopteran insects are not sensitive to RNAi. Here, we present a protocol for knocking down the expression level of target genes by RNAi in Bombyx mori embryos. We describe the preparation of double-stranded RNAs (dsRNAs) of target genes, followed by microinjection of embryos at different developmental stages, with single or mixed dsRNA. Finally, we use RT-qPCR to verify RNAi efficiency. For complete details on the use and execution of this protocol, please refer to Xu et al. (2021)
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spelling pubmed-89082532022-03-11 Knock down of target genes by RNA interference in the embryos of lepidopteran insect, Bombyx mori Xu, Guanfeng Tian, Yulin Peng, Yuling Zheng, Sichun STAR Protoc Protocol RNA interference (RNAi) is a technique used for posttranscriptional gene silencing, but lepidopteran insects are not sensitive to RNAi. Here, we present a protocol for knocking down the expression level of target genes by RNAi in Bombyx mori embryos. We describe the preparation of double-stranded RNAs (dsRNAs) of target genes, followed by microinjection of embryos at different developmental stages, with single or mixed dsRNA. Finally, we use RT-qPCR to verify RNAi efficiency. For complete details on the use and execution of this protocol, please refer to Xu et al. (2021) Elsevier 2022-03-07 /pmc/articles/PMC8908253/ /pubmed/35284831 http://dx.doi.org/10.1016/j.xpro.2022.101219 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Xu, Guanfeng
Tian, Yulin
Peng, Yuling
Zheng, Sichun
Knock down of target genes by RNA interference in the embryos of lepidopteran insect, Bombyx mori
title Knock down of target genes by RNA interference in the embryos of lepidopteran insect, Bombyx mori
title_full Knock down of target genes by RNA interference in the embryos of lepidopteran insect, Bombyx mori
title_fullStr Knock down of target genes by RNA interference in the embryos of lepidopteran insect, Bombyx mori
title_full_unstemmed Knock down of target genes by RNA interference in the embryos of lepidopteran insect, Bombyx mori
title_short Knock down of target genes by RNA interference in the embryos of lepidopteran insect, Bombyx mori
title_sort knock down of target genes by rna interference in the embryos of lepidopteran insect, bombyx mori
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8908253/
https://www.ncbi.nlm.nih.gov/pubmed/35284831
http://dx.doi.org/10.1016/j.xpro.2022.101219
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