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Promoting role of pentraxin-3 in esophageal squamous cell carcinoma

Pentraxin 3 (PTX3) is an inflammatory molecule that is closely related to the proliferation, invasion, and metastasis of cancer. In order to explore the role of PTX3 in the occurrence and development of esophageal carcinoma (ESCA), we modified the PTX3 gene in ESCA cell lines to obtain the model of...

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Autores principales: Fan, Zhirui, Zheng, Yuanyuan, Li, Xiaoli, Deng, Xiaoming, Ba, Yan, Feng, Kun, Su, Jin, Wang, Hui, Suo, Zhenhe, Li, Lifeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Gene & Cell Therapy 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8908267/
https://www.ncbi.nlm.nih.gov/pubmed/35317523
http://dx.doi.org/10.1016/j.omto.2022.02.005
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author Fan, Zhirui
Zheng, Yuanyuan
Li, Xiaoli
Deng, Xiaoming
Ba, Yan
Feng, Kun
Su, Jin
Wang, Hui
Suo, Zhenhe
Li, Lifeng
author_facet Fan, Zhirui
Zheng, Yuanyuan
Li, Xiaoli
Deng, Xiaoming
Ba, Yan
Feng, Kun
Su, Jin
Wang, Hui
Suo, Zhenhe
Li, Lifeng
author_sort Fan, Zhirui
collection PubMed
description Pentraxin 3 (PTX3) is an inflammatory molecule that is closely related to the proliferation, invasion, and metastasis of cancer. In order to explore the role of PTX3 in the occurrence and development of esophageal carcinoma (ESCA), we modified the PTX3 gene in ESCA cell lines to obtain the model of gene knockout and overexpression and studied cell proliferation, cycle, apoptosis, migration ability, energy metabolism, and sensitivity to chemotherapy and radiotherapy. We observed the increase in cell proliferation, cycle, apoptosis, migration ability, and sensitivity to chemotherapy and radiotherapy in the PTX3 knockout model, while in the PTX3 overexpression model, these phenomena were significantly reduced. Knockout of the PTX3 also resulted in decreased cell glycolysis and increased oxidative phosphorylation, which is consistent with other findings that PTX3 affects the tumorigenic ability of cells and their sensitivity to docetaxel. In ESCA, SOX9 directly regulates the expression of PTX3, while human leukocyte antigen (HLA)-system-related genes are significantly up-regulated when lacking PTX3. These results indicate that SOX9 may play a crucial role in regulating PTX3 and affecting the HLA system in ESCA.
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spelling pubmed-89082672022-03-21 Promoting role of pentraxin-3 in esophageal squamous cell carcinoma Fan, Zhirui Zheng, Yuanyuan Li, Xiaoli Deng, Xiaoming Ba, Yan Feng, Kun Su, Jin Wang, Hui Suo, Zhenhe Li, Lifeng Mol Ther Oncolytics Original Article Pentraxin 3 (PTX3) is an inflammatory molecule that is closely related to the proliferation, invasion, and metastasis of cancer. In order to explore the role of PTX3 in the occurrence and development of esophageal carcinoma (ESCA), we modified the PTX3 gene in ESCA cell lines to obtain the model of gene knockout and overexpression and studied cell proliferation, cycle, apoptosis, migration ability, energy metabolism, and sensitivity to chemotherapy and radiotherapy. We observed the increase in cell proliferation, cycle, apoptosis, migration ability, and sensitivity to chemotherapy and radiotherapy in the PTX3 knockout model, while in the PTX3 overexpression model, these phenomena were significantly reduced. Knockout of the PTX3 also resulted in decreased cell glycolysis and increased oxidative phosphorylation, which is consistent with other findings that PTX3 affects the tumorigenic ability of cells and their sensitivity to docetaxel. In ESCA, SOX9 directly regulates the expression of PTX3, while human leukocyte antigen (HLA)-system-related genes are significantly up-regulated when lacking PTX3. These results indicate that SOX9 may play a crucial role in regulating PTX3 and affecting the HLA system in ESCA. American Society of Gene & Cell Therapy 2022-02-14 /pmc/articles/PMC8908267/ /pubmed/35317523 http://dx.doi.org/10.1016/j.omto.2022.02.005 Text en © 2022. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Fan, Zhirui
Zheng, Yuanyuan
Li, Xiaoli
Deng, Xiaoming
Ba, Yan
Feng, Kun
Su, Jin
Wang, Hui
Suo, Zhenhe
Li, Lifeng
Promoting role of pentraxin-3 in esophageal squamous cell carcinoma
title Promoting role of pentraxin-3 in esophageal squamous cell carcinoma
title_full Promoting role of pentraxin-3 in esophageal squamous cell carcinoma
title_fullStr Promoting role of pentraxin-3 in esophageal squamous cell carcinoma
title_full_unstemmed Promoting role of pentraxin-3 in esophageal squamous cell carcinoma
title_short Promoting role of pentraxin-3 in esophageal squamous cell carcinoma
title_sort promoting role of pentraxin-3 in esophageal squamous cell carcinoma
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8908267/
https://www.ncbi.nlm.nih.gov/pubmed/35317523
http://dx.doi.org/10.1016/j.omto.2022.02.005
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