Cargando…
Increasing Severity of Spinal Cord Injury Results in Microglia/Macrophages With Annular-Shaped Morphology and No Change in Expression of CD40 and Tumor Growth Factor-β During the Chronic Post-injury Stage
Determination of the quantitative composition of phenotypically and morphologically different populations of resident microglia and infiltrating macrophages in spinal cord injury (SCI) of various degrees of severity could lead to much needed novel therapeutic interventions in neurotrauma. In this re...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8908449/ https://www.ncbi.nlm.nih.gov/pubmed/35282656 http://dx.doi.org/10.3389/fnmol.2021.802558 |
_version_ | 1784665879858905088 |
---|---|
author | Akhmetzyanova, Elvira Ruslanovna Timofeeva, Anna Viktorovna Sabirov, Davran Khudaishukurovich Kostennikov, Alexander Alexandrovich Rogozhin, Alexander Alexandrovich James, Victoria Arkhipova, S. S. Rizvanov, Albert Anatolevich Mukhamedshina, Yana Olegovna |
author_facet | Akhmetzyanova, Elvira Ruslanovna Timofeeva, Anna Viktorovna Sabirov, Davran Khudaishukurovich Kostennikov, Alexander Alexandrovich Rogozhin, Alexander Alexandrovich James, Victoria Arkhipova, S. S. Rizvanov, Albert Anatolevich Mukhamedshina, Yana Olegovna |
author_sort | Akhmetzyanova, Elvira Ruslanovna |
collection | PubMed |
description | Determination of the quantitative composition of phenotypically and morphologically different populations of resident microglia and infiltrating macrophages in spinal cord injury (SCI) of various degrees of severity could lead to much needed novel therapeutic interventions in neurotrauma. In this regard, we investigated the CD40 and TGF-β expressing populations of microglia/macrophages and their morphological states in a rat model of SCI of varying severity. We are the first to describe the annular-shaped microglia/macrophages, the morphology of which was formed due to the spatial orientation of the processes that form round or oval micro-territories, which include disintegrating myelin fibers. This type of cell morphology was found only in the injured spinal cord and mainly in the white matter. At the same time, an assessment of the number of annular-shaped microglia/macrophages and the diameter of micro-territories formed by their processes showed an elevation in these indicators as the severity of SCI increased. While we did not find significant quantitative changes in the populations of Iba1(+)/CD40(+) and Iba1(+)/TGF-β(+) microglia/macrophages with increased severity of SCI in the chronic period (60 dpi), we did determine changes in the expression of cytokines and mRNAs of genes-encoding microglial marker proteins, finding the greatest changes on days 7 and 14 after SCI between experimental groups with varying severity. |
format | Online Article Text |
id | pubmed-8908449 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-89084492022-03-11 Increasing Severity of Spinal Cord Injury Results in Microglia/Macrophages With Annular-Shaped Morphology and No Change in Expression of CD40 and Tumor Growth Factor-β During the Chronic Post-injury Stage Akhmetzyanova, Elvira Ruslanovna Timofeeva, Anna Viktorovna Sabirov, Davran Khudaishukurovich Kostennikov, Alexander Alexandrovich Rogozhin, Alexander Alexandrovich James, Victoria Arkhipova, S. S. Rizvanov, Albert Anatolevich Mukhamedshina, Yana Olegovna Front Mol Neurosci Neuroscience Determination of the quantitative composition of phenotypically and morphologically different populations of resident microglia and infiltrating macrophages in spinal cord injury (SCI) of various degrees of severity could lead to much needed novel therapeutic interventions in neurotrauma. In this regard, we investigated the CD40 and TGF-β expressing populations of microglia/macrophages and their morphological states in a rat model of SCI of varying severity. We are the first to describe the annular-shaped microglia/macrophages, the morphology of which was formed due to the spatial orientation of the processes that form round or oval micro-territories, which include disintegrating myelin fibers. This type of cell morphology was found only in the injured spinal cord and mainly in the white matter. At the same time, an assessment of the number of annular-shaped microglia/macrophages and the diameter of micro-territories formed by their processes showed an elevation in these indicators as the severity of SCI increased. While we did not find significant quantitative changes in the populations of Iba1(+)/CD40(+) and Iba1(+)/TGF-β(+) microglia/macrophages with increased severity of SCI in the chronic period (60 dpi), we did determine changes in the expression of cytokines and mRNAs of genes-encoding microglial marker proteins, finding the greatest changes on days 7 and 14 after SCI between experimental groups with varying severity. Frontiers Media S.A. 2022-02-24 /pmc/articles/PMC8908449/ /pubmed/35282656 http://dx.doi.org/10.3389/fnmol.2021.802558 Text en Copyright © 2022 Akhmetzyanova, Timofeeva, Sabirov, Kostennikov, Rogozhin, James, Arkhipova, Rizvanov and Mukhamedshina. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Neuroscience Akhmetzyanova, Elvira Ruslanovna Timofeeva, Anna Viktorovna Sabirov, Davran Khudaishukurovich Kostennikov, Alexander Alexandrovich Rogozhin, Alexander Alexandrovich James, Victoria Arkhipova, S. S. Rizvanov, Albert Anatolevich Mukhamedshina, Yana Olegovna Increasing Severity of Spinal Cord Injury Results in Microglia/Macrophages With Annular-Shaped Morphology and No Change in Expression of CD40 and Tumor Growth Factor-β During the Chronic Post-injury Stage |
title | Increasing Severity of Spinal Cord Injury Results in Microglia/Macrophages With Annular-Shaped Morphology and No Change in Expression of CD40 and Tumor Growth Factor-β During the Chronic Post-injury Stage |
title_full | Increasing Severity of Spinal Cord Injury Results in Microglia/Macrophages With Annular-Shaped Morphology and No Change in Expression of CD40 and Tumor Growth Factor-β During the Chronic Post-injury Stage |
title_fullStr | Increasing Severity of Spinal Cord Injury Results in Microglia/Macrophages With Annular-Shaped Morphology and No Change in Expression of CD40 and Tumor Growth Factor-β During the Chronic Post-injury Stage |
title_full_unstemmed | Increasing Severity of Spinal Cord Injury Results in Microglia/Macrophages With Annular-Shaped Morphology and No Change in Expression of CD40 and Tumor Growth Factor-β During the Chronic Post-injury Stage |
title_short | Increasing Severity of Spinal Cord Injury Results in Microglia/Macrophages With Annular-Shaped Morphology and No Change in Expression of CD40 and Tumor Growth Factor-β During the Chronic Post-injury Stage |
title_sort | increasing severity of spinal cord injury results in microglia/macrophages with annular-shaped morphology and no change in expression of cd40 and tumor growth factor-β during the chronic post-injury stage |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8908449/ https://www.ncbi.nlm.nih.gov/pubmed/35282656 http://dx.doi.org/10.3389/fnmol.2021.802558 |
work_keys_str_mv | AT akhmetzyanovaelviraruslanovna increasingseverityofspinalcordinjuryresultsinmicrogliamacrophageswithannularshapedmorphologyandnochangeinexpressionofcd40andtumorgrowthfactorbduringthechronicpostinjurystage AT timofeevaannaviktorovna increasingseverityofspinalcordinjuryresultsinmicrogliamacrophageswithannularshapedmorphologyandnochangeinexpressionofcd40andtumorgrowthfactorbduringthechronicpostinjurystage AT sabirovdavrankhudaishukurovich increasingseverityofspinalcordinjuryresultsinmicrogliamacrophageswithannularshapedmorphologyandnochangeinexpressionofcd40andtumorgrowthfactorbduringthechronicpostinjurystage AT kostennikovalexanderalexandrovich increasingseverityofspinalcordinjuryresultsinmicrogliamacrophageswithannularshapedmorphologyandnochangeinexpressionofcd40andtumorgrowthfactorbduringthechronicpostinjurystage AT rogozhinalexanderalexandrovich increasingseverityofspinalcordinjuryresultsinmicrogliamacrophageswithannularshapedmorphologyandnochangeinexpressionofcd40andtumorgrowthfactorbduringthechronicpostinjurystage AT jamesvictoria increasingseverityofspinalcordinjuryresultsinmicrogliamacrophageswithannularshapedmorphologyandnochangeinexpressionofcd40andtumorgrowthfactorbduringthechronicpostinjurystage AT arkhipovass increasingseverityofspinalcordinjuryresultsinmicrogliamacrophageswithannularshapedmorphologyandnochangeinexpressionofcd40andtumorgrowthfactorbduringthechronicpostinjurystage AT rizvanovalbertanatolevich increasingseverityofspinalcordinjuryresultsinmicrogliamacrophageswithannularshapedmorphologyandnochangeinexpressionofcd40andtumorgrowthfactorbduringthechronicpostinjurystage AT mukhamedshinayanaolegovna increasingseverityofspinalcordinjuryresultsinmicrogliamacrophageswithannularshapedmorphologyandnochangeinexpressionofcd40andtumorgrowthfactorbduringthechronicpostinjurystage |