Relevance of Aquaporins for Gamete Function and Cryopreservation

SIMPLE SUMMARY: The interaction between cells and the extracellular medium is of great importance; changes in medium composition can drive water movement across plasma membranes. Aquaporins (AQPs) are membrane channels involved in the transport of water and some solutes across membranes. When sperm enter the female reproductive tract after ejaculation, they encounter a drastic change in extracellular composition, which leads to water flowing across the plasma membrane. This triggers a series of events that are crucial to allowing fertilization to take place, such as regulation of sperm motility. In the context of assisted reproduction techniques (ART), long-term storage of gametes is sometimes required, and, during cryopreservation, these cells undergo drastic changes in extracellular medium composition. As a result, AQPs are crucial in both sperm and oocytes during this process. Cryopreservation is of considerable importance for fertility preservation in livestock, endangered species and for individuals undergoing certain medical treatments that compromise their fertility. Further research to fully elucidate the roles and underlying mechanisms of AQPs in mammalian sperm is therefore warranted. ABSTRACT: The interaction between cells and the extracellular medium is of great importance, and drastic changes in extracellular solute concentrations drive water movement across the plasma membrane. Aquaporins (AQPs) are a family of transmembrane channels that allow the transport of water and small solutes across cell membranes. Different members of this family have been identified in gametes. In sperm, they are relevant to osmoadaptation after entering the female reproductive tract, which is crucial for sperm motility activation and capacitation and, thus, for their fertilizing ability. In addition, they are relevant during the cryopreservation process, since some members of this family are also permeable to glycerol, one of the most frequently used cryoprotective agents in livestock. Regarding oocytes, AQPs are very important in their maturation but also during cryopreservation. Further research to define the exact sets of AQPs that are present in oocytes from different species is needed, since the available literature envisages certain AQPs and their roles but does not provide complete information on the whole set of AQPs. This is of considerable importance because, in sperm, specific AQPs are known to compensate the role of non-functional members.