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Maintenance of Chronological Aging Features in Culture of Normal Human Dermal Fibroblasts from Old Donors

Chronological aging is defined as a time-dependent decline of tissue homeostasis which severely impacts skin. Understanding the mechanisms of skin aging is an active research area limited by the lack of relevant in vitro models. Being a component of aging, replicative or stress-induced senescence is...

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Autores principales: Rorteau, Julie, Chevalier, Fabien P., Bonnet, Sébastien, Barthélemy, Théo, Lopez-Gaydon, Amandine, Martin, Lisa S., Bechetoille, Nicolas, Lamartine, Jérôme
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8909060/
https://www.ncbi.nlm.nih.gov/pubmed/35269480
http://dx.doi.org/10.3390/cells11050858
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author Rorteau, Julie
Chevalier, Fabien P.
Bonnet, Sébastien
Barthélemy, Théo
Lopez-Gaydon, Amandine
Martin, Lisa S.
Bechetoille, Nicolas
Lamartine, Jérôme
author_facet Rorteau, Julie
Chevalier, Fabien P.
Bonnet, Sébastien
Barthélemy, Théo
Lopez-Gaydon, Amandine
Martin, Lisa S.
Bechetoille, Nicolas
Lamartine, Jérôme
author_sort Rorteau, Julie
collection PubMed
description Chronological aging is defined as a time-dependent decline of tissue homeostasis which severely impacts skin. Understanding the mechanisms of skin aging is an active research area limited by the lack of relevant in vitro models. Being a component of aging, replicative or stress-induced senescence is repeatedly used to mimic skin aging in vitro, thus presenting only a partial view of the complexity of aging. Herein, we aimed to clarify whether primary normal human dermal fibroblasts retained age-related characteristics when cultured in 2D monolayer, and could be used as a relevant model for aging research. We compared three groups of fibroblasts isolated from different aged donors. We observed strongly decreased population doubling capacities, a reduced clonogenic ability, an impairment in extracellular matrix production together with modifications of respiratory metabolism with an increase in age. These disruptions were particularly marked when comparing fibroblasts isolated from old individuals (over 70 years old) to those isolated from young individuals (18–37 years old), while cells from middle-aged donors exhibited an intermediate profile. These alterations of cell features can be related to the signs of dermis aging, thus showing that cultured primary cells indeed retain some characteristics of the original tissue from which they were extracted.
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spelling pubmed-89090602022-03-11 Maintenance of Chronological Aging Features in Culture of Normal Human Dermal Fibroblasts from Old Donors Rorteau, Julie Chevalier, Fabien P. Bonnet, Sébastien Barthélemy, Théo Lopez-Gaydon, Amandine Martin, Lisa S. Bechetoille, Nicolas Lamartine, Jérôme Cells Article Chronological aging is defined as a time-dependent decline of tissue homeostasis which severely impacts skin. Understanding the mechanisms of skin aging is an active research area limited by the lack of relevant in vitro models. Being a component of aging, replicative or stress-induced senescence is repeatedly used to mimic skin aging in vitro, thus presenting only a partial view of the complexity of aging. Herein, we aimed to clarify whether primary normal human dermal fibroblasts retained age-related characteristics when cultured in 2D monolayer, and could be used as a relevant model for aging research. We compared three groups of fibroblasts isolated from different aged donors. We observed strongly decreased population doubling capacities, a reduced clonogenic ability, an impairment in extracellular matrix production together with modifications of respiratory metabolism with an increase in age. These disruptions were particularly marked when comparing fibroblasts isolated from old individuals (over 70 years old) to those isolated from young individuals (18–37 years old), while cells from middle-aged donors exhibited an intermediate profile. These alterations of cell features can be related to the signs of dermis aging, thus showing that cultured primary cells indeed retain some characteristics of the original tissue from which they were extracted. MDPI 2022-03-02 /pmc/articles/PMC8909060/ /pubmed/35269480 http://dx.doi.org/10.3390/cells11050858 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rorteau, Julie
Chevalier, Fabien P.
Bonnet, Sébastien
Barthélemy, Théo
Lopez-Gaydon, Amandine
Martin, Lisa S.
Bechetoille, Nicolas
Lamartine, Jérôme
Maintenance of Chronological Aging Features in Culture of Normal Human Dermal Fibroblasts from Old Donors
title Maintenance of Chronological Aging Features in Culture of Normal Human Dermal Fibroblasts from Old Donors
title_full Maintenance of Chronological Aging Features in Culture of Normal Human Dermal Fibroblasts from Old Donors
title_fullStr Maintenance of Chronological Aging Features in Culture of Normal Human Dermal Fibroblasts from Old Donors
title_full_unstemmed Maintenance of Chronological Aging Features in Culture of Normal Human Dermal Fibroblasts from Old Donors
title_short Maintenance of Chronological Aging Features in Culture of Normal Human Dermal Fibroblasts from Old Donors
title_sort maintenance of chronological aging features in culture of normal human dermal fibroblasts from old donors
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8909060/
https://www.ncbi.nlm.nih.gov/pubmed/35269480
http://dx.doi.org/10.3390/cells11050858
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