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Human Mesenchymal Stem Cells as a Carrier for a Cell-Mediated Drug Delivery

A number of preclinical and clinical studies have demonstrated the efficiency of mesenchymal stromal cells to serve as an excellent base for a cell-mediated drug delivery system. Cell-based targeted drug delivery has received much attention as a system to facilitate the uptake a nd transfer of activ...

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Autores principales: Litvinova, L. S., Shupletsova, V. V., Khaziakhmatova, O. G., Daminova, A. G., Kudryavtseva, V. L., Yurova, K. A., Malashchenko, V. V., Todosenko, N. M., Popova, V., Litvinov, R. I., Korotkova, E. I., Sukhorukov, G. B., Gow, A. J., Weissman, D., Atochina-Vasserman, E. N., Khlusov, I. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8909129/
https://www.ncbi.nlm.nih.gov/pubmed/35284410
http://dx.doi.org/10.3389/fbioe.2022.796111
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author Litvinova, L. S.
Shupletsova, V. V.
Khaziakhmatova, O. G.
Daminova, A. G.
Kudryavtseva, V. L.
Yurova, K. A.
Malashchenko, V. V.
Todosenko, N. M.
Popova, V.
Litvinov, R. I.
Korotkova, E. I.
Sukhorukov, G. B.
Gow, A. J.
Weissman, D.
Atochina-Vasserman, E. N.
Khlusov, I. A.
author_facet Litvinova, L. S.
Shupletsova, V. V.
Khaziakhmatova, O. G.
Daminova, A. G.
Kudryavtseva, V. L.
Yurova, K. A.
Malashchenko, V. V.
Todosenko, N. M.
Popova, V.
Litvinov, R. I.
Korotkova, E. I.
Sukhorukov, G. B.
Gow, A. J.
Weissman, D.
Atochina-Vasserman, E. N.
Khlusov, I. A.
author_sort Litvinova, L. S.
collection PubMed
description A number of preclinical and clinical studies have demonstrated the efficiency of mesenchymal stromal cells to serve as an excellent base for a cell-mediated drug delivery system. Cell-based targeted drug delivery has received much attention as a system to facilitate the uptake a nd transfer of active substances to specific organs and tissues with high efficiency. Human mesenchymal stem cells (MSCs) are attracting increased interest as a promising tool for cell-based therapy due to their high proliferative capacity, multi-potency, and anti-inflammatory and immunomodulatory properties. In particular, these cells are potentially suitable for use as encapsulated drug transporters to sites of inflammation. Here, we studied the in vitro effects of incorporating synthetic polymer microcapsules at various microcapsule-to-cell ratios on the morphology, ultrastructure, cytokine profile, and migration ability of human adipose-derived MSCs at various time points post-phagocytosis. The data show that under appropriate conditions, human MSCs can be efficiently loaded with synthesized microcapsules without damaging the cell’s structural integrity with unexpressed cytokine secretion, retained motility, and ability to migrate through 8 μm pores. Thus, the strategy of using human MSCs as a delivery vehicle for transferring microcapsules, containing bioactive material, across the tissue–blood or tumor–blood barriers to facilitate the treatment of stroke, cancer, or inflammatory diseases may open a new therapeutic perspective.
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spelling pubmed-89091292022-03-11 Human Mesenchymal Stem Cells as a Carrier for a Cell-Mediated Drug Delivery Litvinova, L. S. Shupletsova, V. V. Khaziakhmatova, O. G. Daminova, A. G. Kudryavtseva, V. L. Yurova, K. A. Malashchenko, V. V. Todosenko, N. M. Popova, V. Litvinov, R. I. Korotkova, E. I. Sukhorukov, G. B. Gow, A. J. Weissman, D. Atochina-Vasserman, E. N. Khlusov, I. A. Front Bioeng Biotechnol Bioengineering and Biotechnology A number of preclinical and clinical studies have demonstrated the efficiency of mesenchymal stromal cells to serve as an excellent base for a cell-mediated drug delivery system. Cell-based targeted drug delivery has received much attention as a system to facilitate the uptake a nd transfer of active substances to specific organs and tissues with high efficiency. Human mesenchymal stem cells (MSCs) are attracting increased interest as a promising tool for cell-based therapy due to their high proliferative capacity, multi-potency, and anti-inflammatory and immunomodulatory properties. In particular, these cells are potentially suitable for use as encapsulated drug transporters to sites of inflammation. Here, we studied the in vitro effects of incorporating synthetic polymer microcapsules at various microcapsule-to-cell ratios on the morphology, ultrastructure, cytokine profile, and migration ability of human adipose-derived MSCs at various time points post-phagocytosis. The data show that under appropriate conditions, human MSCs can be efficiently loaded with synthesized microcapsules without damaging the cell’s structural integrity with unexpressed cytokine secretion, retained motility, and ability to migrate through 8 μm pores. Thus, the strategy of using human MSCs as a delivery vehicle for transferring microcapsules, containing bioactive material, across the tissue–blood or tumor–blood barriers to facilitate the treatment of stroke, cancer, or inflammatory diseases may open a new therapeutic perspective. Frontiers Media S.A. 2022-02-24 /pmc/articles/PMC8909129/ /pubmed/35284410 http://dx.doi.org/10.3389/fbioe.2022.796111 Text en Copyright © 2022 Litvinova, Shupletsova, Khaziakhmatova, Daminova, Kudryavtseva, Yurova, Malashchenko, Todosenko, Popova, Litvinov, Korotkova, Sukhorukov, Gow, Weissman, Atochina-Vasserman and Khlusov. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Litvinova, L. S.
Shupletsova, V. V.
Khaziakhmatova, O. G.
Daminova, A. G.
Kudryavtseva, V. L.
Yurova, K. A.
Malashchenko, V. V.
Todosenko, N. M.
Popova, V.
Litvinov, R. I.
Korotkova, E. I.
Sukhorukov, G. B.
Gow, A. J.
Weissman, D.
Atochina-Vasserman, E. N.
Khlusov, I. A.
Human Mesenchymal Stem Cells as a Carrier for a Cell-Mediated Drug Delivery
title Human Mesenchymal Stem Cells as a Carrier for a Cell-Mediated Drug Delivery
title_full Human Mesenchymal Stem Cells as a Carrier for a Cell-Mediated Drug Delivery
title_fullStr Human Mesenchymal Stem Cells as a Carrier for a Cell-Mediated Drug Delivery
title_full_unstemmed Human Mesenchymal Stem Cells as a Carrier for a Cell-Mediated Drug Delivery
title_short Human Mesenchymal Stem Cells as a Carrier for a Cell-Mediated Drug Delivery
title_sort human mesenchymal stem cells as a carrier for a cell-mediated drug delivery
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8909129/
https://www.ncbi.nlm.nih.gov/pubmed/35284410
http://dx.doi.org/10.3389/fbioe.2022.796111
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