Cargando…

Notoginsenoside R1 (NG-R1) Promoted Lymphatic Drainage Function to Ameliorating Rheumatoid Arthritis in TNF-Tg Mice by Suppressing NF-κB Signaling Pathway

Rheumatoid arthritis (RA) is a chronic autoimmune disease that is primarily characterized by synovial inflammation. Our previous studies demonstrated that the lymphatic system is critical for the development and maintenance of RA disease, and sufficient lymph drainage helps to improve joint inflamma...

Descripción completa

Detalles Bibliográficos
Autores principales: Jiao, Danli, Liu, Yang, Hou, Tong, Xu, Hao, Wang, Xiaoyun, Shi, Qi, Wang, Yongjun, Xing, Qiujuan, Liang, Qianqian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8909130/
https://www.ncbi.nlm.nih.gov/pubmed/35280253
http://dx.doi.org/10.3389/fphar.2021.730579
_version_ 1784666047382552576
author Jiao, Danli
Liu, Yang
Hou, Tong
Xu, Hao
Wang, Xiaoyun
Shi, Qi
Wang, Yongjun
Xing, Qiujuan
Liang, Qianqian
author_facet Jiao, Danli
Liu, Yang
Hou, Tong
Xu, Hao
Wang, Xiaoyun
Shi, Qi
Wang, Yongjun
Xing, Qiujuan
Liang, Qianqian
author_sort Jiao, Danli
collection PubMed
description Rheumatoid arthritis (RA) is a chronic autoimmune disease that is primarily characterized by synovial inflammation. Our previous studies demonstrated that the lymphatic system is critical for the development and maintenance of RA disease, and sufficient lymph drainage helps to improve joint inflammation. In this study, we found that NG-R1, the main active component in the traditional Chinese medicinal herb Sanchi, activating lymphatic function can attenuate synovial inflammation. According to histopathological staining of ankle sections, NG-R1 significantly decreased the area of inflammation and reduced bone destruction of ankle joints in TNF-Tg mice. Near infrared-indocyanine green (NIR-ICG) lymphatic imaging system has shown that NG-R1 significantly improved the lymphatic drainage function. However, the molecular mechanism of its activity is not properly understood. Our in-depth study demonstrates that NG-R1 reduced the inflammatory cytokine production of lymphatic endothelial cells (LECs) stimulated by TNF-α, and the mechanism ameliorated the phosphorylation of IKKα/β and p65, and the translocation of p65 into the nucleus. In summary, this study proved that NG-R1 promoted lymphatic drainage function to ameliorating rheumatoid arthritis in TNF-Tg mice by suppressing NF-κB signaling pathway.
format Online
Article
Text
id pubmed-8909130
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-89091302022-03-11 Notoginsenoside R1 (NG-R1) Promoted Lymphatic Drainage Function to Ameliorating Rheumatoid Arthritis in TNF-Tg Mice by Suppressing NF-κB Signaling Pathway Jiao, Danli Liu, Yang Hou, Tong Xu, Hao Wang, Xiaoyun Shi, Qi Wang, Yongjun Xing, Qiujuan Liang, Qianqian Front Pharmacol Pharmacology Rheumatoid arthritis (RA) is a chronic autoimmune disease that is primarily characterized by synovial inflammation. Our previous studies demonstrated that the lymphatic system is critical for the development and maintenance of RA disease, and sufficient lymph drainage helps to improve joint inflammation. In this study, we found that NG-R1, the main active component in the traditional Chinese medicinal herb Sanchi, activating lymphatic function can attenuate synovial inflammation. According to histopathological staining of ankle sections, NG-R1 significantly decreased the area of inflammation and reduced bone destruction of ankle joints in TNF-Tg mice. Near infrared-indocyanine green (NIR-ICG) lymphatic imaging system has shown that NG-R1 significantly improved the lymphatic drainage function. However, the molecular mechanism of its activity is not properly understood. Our in-depth study demonstrates that NG-R1 reduced the inflammatory cytokine production of lymphatic endothelial cells (LECs) stimulated by TNF-α, and the mechanism ameliorated the phosphorylation of IKKα/β and p65, and the translocation of p65 into the nucleus. In summary, this study proved that NG-R1 promoted lymphatic drainage function to ameliorating rheumatoid arthritis in TNF-Tg mice by suppressing NF-κB signaling pathway. Frontiers Media S.A. 2022-02-24 /pmc/articles/PMC8909130/ /pubmed/35280253 http://dx.doi.org/10.3389/fphar.2021.730579 Text en Copyright © 2022 Jiao, Liu, Hou, Xu, Wang, Shi, Wang, Xing and Liang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Jiao, Danli
Liu, Yang
Hou, Tong
Xu, Hao
Wang, Xiaoyun
Shi, Qi
Wang, Yongjun
Xing, Qiujuan
Liang, Qianqian
Notoginsenoside R1 (NG-R1) Promoted Lymphatic Drainage Function to Ameliorating Rheumatoid Arthritis in TNF-Tg Mice by Suppressing NF-κB Signaling Pathway
title Notoginsenoside R1 (NG-R1) Promoted Lymphatic Drainage Function to Ameliorating Rheumatoid Arthritis in TNF-Tg Mice by Suppressing NF-κB Signaling Pathway
title_full Notoginsenoside R1 (NG-R1) Promoted Lymphatic Drainage Function to Ameliorating Rheumatoid Arthritis in TNF-Tg Mice by Suppressing NF-κB Signaling Pathway
title_fullStr Notoginsenoside R1 (NG-R1) Promoted Lymphatic Drainage Function to Ameliorating Rheumatoid Arthritis in TNF-Tg Mice by Suppressing NF-κB Signaling Pathway
title_full_unstemmed Notoginsenoside R1 (NG-R1) Promoted Lymphatic Drainage Function to Ameliorating Rheumatoid Arthritis in TNF-Tg Mice by Suppressing NF-κB Signaling Pathway
title_short Notoginsenoside R1 (NG-R1) Promoted Lymphatic Drainage Function to Ameliorating Rheumatoid Arthritis in TNF-Tg Mice by Suppressing NF-κB Signaling Pathway
title_sort notoginsenoside r1 (ng-r1) promoted lymphatic drainage function to ameliorating rheumatoid arthritis in tnf-tg mice by suppressing nf-κb signaling pathway
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8909130/
https://www.ncbi.nlm.nih.gov/pubmed/35280253
http://dx.doi.org/10.3389/fphar.2021.730579
work_keys_str_mv AT jiaodanli notoginsenosider1ngr1promotedlymphaticdrainagefunctiontoamelioratingrheumatoidarthritisintnftgmicebysuppressingnfkbsignalingpathway
AT liuyang notoginsenosider1ngr1promotedlymphaticdrainagefunctiontoamelioratingrheumatoidarthritisintnftgmicebysuppressingnfkbsignalingpathway
AT houtong notoginsenosider1ngr1promotedlymphaticdrainagefunctiontoamelioratingrheumatoidarthritisintnftgmicebysuppressingnfkbsignalingpathway
AT xuhao notoginsenosider1ngr1promotedlymphaticdrainagefunctiontoamelioratingrheumatoidarthritisintnftgmicebysuppressingnfkbsignalingpathway
AT wangxiaoyun notoginsenosider1ngr1promotedlymphaticdrainagefunctiontoamelioratingrheumatoidarthritisintnftgmicebysuppressingnfkbsignalingpathway
AT shiqi notoginsenosider1ngr1promotedlymphaticdrainagefunctiontoamelioratingrheumatoidarthritisintnftgmicebysuppressingnfkbsignalingpathway
AT wangyongjun notoginsenosider1ngr1promotedlymphaticdrainagefunctiontoamelioratingrheumatoidarthritisintnftgmicebysuppressingnfkbsignalingpathway
AT xingqiujuan notoginsenosider1ngr1promotedlymphaticdrainagefunctiontoamelioratingrheumatoidarthritisintnftgmicebysuppressingnfkbsignalingpathway
AT liangqianqian notoginsenosider1ngr1promotedlymphaticdrainagefunctiontoamelioratingrheumatoidarthritisintnftgmicebysuppressingnfkbsignalingpathway