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Evaluation of Osteogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cell on Highly Porous Polycaprolactone Scaffold Reinforced With Layered Double Hydroxides Nanoclay

In recent decades, bone tissue engineering has had an effective role in introducing orthopedic implants. In this regard, polymeric scaffolds reinforced with bioactive nanomaterials can offer great potential in tissue engineering implants for replacing bone loss in patients. In this study, the therma...

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Autores principales: Enderami, Seyedeh Elnaz, Shafiei, Seyedeh Sara, Shamsara, Mehdi, Enderami, Seyed Ehsan, Rostamian Tabari, Abolfazl
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8909131/
https://www.ncbi.nlm.nih.gov/pubmed/35284421
http://dx.doi.org/10.3389/fbioe.2022.805969
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author Enderami, Seyedeh Elnaz
Shafiei, Seyedeh Sara
Shamsara, Mehdi
Enderami, Seyed Ehsan
Rostamian Tabari, Abolfazl
author_facet Enderami, Seyedeh Elnaz
Shafiei, Seyedeh Sara
Shamsara, Mehdi
Enderami, Seyed Ehsan
Rostamian Tabari, Abolfazl
author_sort Enderami, Seyedeh Elnaz
collection PubMed
description In recent decades, bone tissue engineering has had an effective role in introducing orthopedic implants. In this regard, polymeric scaffolds reinforced with bioactive nanomaterials can offer great potential in tissue engineering implants for replacing bone loss in patients. In this study, the thermally induced phase separation method was used to fabricate three-dimensional highly porous scaffolds made of layered double hydroxide (LDH)/polycaprolactone (PCL) nanocomposites with varied LDH contents ranging from 0.1 wt.% to 10 wt.%. The Phase identification, morphology, and elemental composition were studied using X-ray diffraction, scanning electron microscopy, and energy-dispersive X-ray spectroscopy, respectively. Interconnected pores ranging from 5 to 150 µm were detected in all samples. The results revealed that the inclusion of LDH to PCL scaffold reinforced mechanical strength and compressive modulus increased from 0.6418 to 1.3251 for the pure PCL and PCL + LDH (1 Wt.%) scaffolds, respectively. Also, thermal stability, degradation rate, and biomineralization especially in comparison with the pure PCL were enhanced. Adhesion, viability, and proliferation of human bone marrow-derived mesenchymal stem cells (hBMSCs) seeded on PCL + LDH scaffolds were improved as compared to the pure PCL. Furthermore, the addition of LDH resulted in the increased mineral deposition as well as expression of ALP and RUNX2 osteogenic genes in terms of differentiation. All in all, our findings revealed that PCL + LDH (1 Wt.%) scaffold might be an ideal choice for 3D scaffold design in bone tissue engineering approaches.
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spelling pubmed-89091312022-03-11 Evaluation of Osteogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cell on Highly Porous Polycaprolactone Scaffold Reinforced With Layered Double Hydroxides Nanoclay Enderami, Seyedeh Elnaz Shafiei, Seyedeh Sara Shamsara, Mehdi Enderami, Seyed Ehsan Rostamian Tabari, Abolfazl Front Bioeng Biotechnol Bioengineering and Biotechnology In recent decades, bone tissue engineering has had an effective role in introducing orthopedic implants. In this regard, polymeric scaffolds reinforced with bioactive nanomaterials can offer great potential in tissue engineering implants for replacing bone loss in patients. In this study, the thermally induced phase separation method was used to fabricate three-dimensional highly porous scaffolds made of layered double hydroxide (LDH)/polycaprolactone (PCL) nanocomposites with varied LDH contents ranging from 0.1 wt.% to 10 wt.%. The Phase identification, morphology, and elemental composition were studied using X-ray diffraction, scanning electron microscopy, and energy-dispersive X-ray spectroscopy, respectively. Interconnected pores ranging from 5 to 150 µm were detected in all samples. The results revealed that the inclusion of LDH to PCL scaffold reinforced mechanical strength and compressive modulus increased from 0.6418 to 1.3251 for the pure PCL and PCL + LDH (1 Wt.%) scaffolds, respectively. Also, thermal stability, degradation rate, and biomineralization especially in comparison with the pure PCL were enhanced. Adhesion, viability, and proliferation of human bone marrow-derived mesenchymal stem cells (hBMSCs) seeded on PCL + LDH scaffolds were improved as compared to the pure PCL. Furthermore, the addition of LDH resulted in the increased mineral deposition as well as expression of ALP and RUNX2 osteogenic genes in terms of differentiation. All in all, our findings revealed that PCL + LDH (1 Wt.%) scaffold might be an ideal choice for 3D scaffold design in bone tissue engineering approaches. Frontiers Media S.A. 2022-02-24 /pmc/articles/PMC8909131/ /pubmed/35284421 http://dx.doi.org/10.3389/fbioe.2022.805969 Text en Copyright © 2022 Enderami, Shafiei, Shamsara, Enderami and Rostamian Tabari. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Enderami, Seyedeh Elnaz
Shafiei, Seyedeh Sara
Shamsara, Mehdi
Enderami, Seyed Ehsan
Rostamian Tabari, Abolfazl
Evaluation of Osteogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cell on Highly Porous Polycaprolactone Scaffold Reinforced With Layered Double Hydroxides Nanoclay
title Evaluation of Osteogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cell on Highly Porous Polycaprolactone Scaffold Reinforced With Layered Double Hydroxides Nanoclay
title_full Evaluation of Osteogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cell on Highly Porous Polycaprolactone Scaffold Reinforced With Layered Double Hydroxides Nanoclay
title_fullStr Evaluation of Osteogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cell on Highly Porous Polycaprolactone Scaffold Reinforced With Layered Double Hydroxides Nanoclay
title_full_unstemmed Evaluation of Osteogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cell on Highly Porous Polycaprolactone Scaffold Reinforced With Layered Double Hydroxides Nanoclay
title_short Evaluation of Osteogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cell on Highly Porous Polycaprolactone Scaffold Reinforced With Layered Double Hydroxides Nanoclay
title_sort evaluation of osteogenic differentiation of bone marrow-derived mesenchymal stem cell on highly porous polycaprolactone scaffold reinforced with layered double hydroxides nanoclay
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8909131/
https://www.ncbi.nlm.nih.gov/pubmed/35284421
http://dx.doi.org/10.3389/fbioe.2022.805969
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