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Contribution of Endothelial Laminin-Binding Integrins to Cellular Processes Associated with Angiogenesis
Endothelial cells engage extracellular matrix and basement membrane components through integrin-mediated adhesion to promote angiogenesis. Angiogenesis involves the sprouting of endothelial cells from pre-existing vessels, their migration into surrounding tissue, the upregulation of angiogenesis-ass...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8909174/ https://www.ncbi.nlm.nih.gov/pubmed/35269439 http://dx.doi.org/10.3390/cells11050816 |
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author | Xu, Hao LaFlamme, Susan E. |
author_facet | Xu, Hao LaFlamme, Susan E. |
author_sort | Xu, Hao |
collection | PubMed |
description | Endothelial cells engage extracellular matrix and basement membrane components through integrin-mediated adhesion to promote angiogenesis. Angiogenesis involves the sprouting of endothelial cells from pre-existing vessels, their migration into surrounding tissue, the upregulation of angiogenesis-associated genes, and the formation of new endothelial tubes. To determine whether the endothelial laminin-binding integrins, α6β4, and α3β1 contribute to these processes, we employed RNAi technology in organotypic angiogenesis assays, as well in migration assays, in vitro. The endothelial depletion of either α6β4 or α3β1 inhibited endothelial sprouting, indicating that these integrins have non-redundant roles in this process. Interestingly, these phenotypes were accompanied by overlapping and distinct changes in the expression of angiogenesis-associated genes. Lastly, depletion of α6β4, but not α3β1, inhibited migration. Taken together, these results suggest that laminin-binding integrins regulate processes associated with angiogenesis by distinct and overlapping mechanisms. |
format | Online Article Text |
id | pubmed-8909174 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-89091742022-03-11 Contribution of Endothelial Laminin-Binding Integrins to Cellular Processes Associated with Angiogenesis Xu, Hao LaFlamme, Susan E. Cells Article Endothelial cells engage extracellular matrix and basement membrane components through integrin-mediated adhesion to promote angiogenesis. Angiogenesis involves the sprouting of endothelial cells from pre-existing vessels, their migration into surrounding tissue, the upregulation of angiogenesis-associated genes, and the formation of new endothelial tubes. To determine whether the endothelial laminin-binding integrins, α6β4, and α3β1 contribute to these processes, we employed RNAi technology in organotypic angiogenesis assays, as well in migration assays, in vitro. The endothelial depletion of either α6β4 or α3β1 inhibited endothelial sprouting, indicating that these integrins have non-redundant roles in this process. Interestingly, these phenotypes were accompanied by overlapping and distinct changes in the expression of angiogenesis-associated genes. Lastly, depletion of α6β4, but not α3β1, inhibited migration. Taken together, these results suggest that laminin-binding integrins regulate processes associated with angiogenesis by distinct and overlapping mechanisms. MDPI 2022-02-26 /pmc/articles/PMC8909174/ /pubmed/35269439 http://dx.doi.org/10.3390/cells11050816 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Xu, Hao LaFlamme, Susan E. Contribution of Endothelial Laminin-Binding Integrins to Cellular Processes Associated with Angiogenesis |
title | Contribution of Endothelial Laminin-Binding Integrins to Cellular Processes Associated with Angiogenesis |
title_full | Contribution of Endothelial Laminin-Binding Integrins to Cellular Processes Associated with Angiogenesis |
title_fullStr | Contribution of Endothelial Laminin-Binding Integrins to Cellular Processes Associated with Angiogenesis |
title_full_unstemmed | Contribution of Endothelial Laminin-Binding Integrins to Cellular Processes Associated with Angiogenesis |
title_short | Contribution of Endothelial Laminin-Binding Integrins to Cellular Processes Associated with Angiogenesis |
title_sort | contribution of endothelial laminin-binding integrins to cellular processes associated with angiogenesis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8909174/ https://www.ncbi.nlm.nih.gov/pubmed/35269439 http://dx.doi.org/10.3390/cells11050816 |
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