The Characteristics and Distribution of α2D-, α2B- and α2C-Adrenoceptor Subtypes in Goats

SIMPLE SUMMARY: α2-Adrenergic receptors mediate many diverse biological effects of the endogenous catecholamines epinephrine and norepinephrine. Three distinct subtypes of α2-adrenergic receptors, α2B, α2C and α2D, have been identified in goats; however, the characteristics and distribution of α2-adrenoceptors in goats remain unclear. Therefore, the aim of our study was to assess the characteristics and distribution of α2-adrenoceptor subtypes in goats. Our study highlights the wide but uneven distribution of α2-adrenoceptor subtypes in goats. Additionally, our study showed that α2D-ceptor has a better analgesic effect in goats than α2B- and α2C-adrenoceptor, whereas α2C-adrenoceptor plays a more important role in thermoregulation than α2B- and α2D-adrenoceptors. ABSTRACT: α2-Adrenegic receptors (α2Rs) are important presynaptic modulators of central noradrenergic function (auto receptors) and postsynaptic mediators of many of the widespread effects of catecholamines and related drugs. Studies have shown that ruminants (such as goats and cattle) express special α2DR subtypes in addition to α2BR and α2CR. Real-time quantitative PCR and Western blotting were used to investigate the distribution and density of α2R in different nuclei of the goat central nervous system, selected regions of the spinal cord (L4-L6), and in various peripheral tissues. α2-AR subtype-specific antibodies were injected intrathecally and intracerebroventricularly into the tested goats to block the corresponding subtype of receptors. Pain threshold and physiological parameters were evaluated to explore the functional characteristics of α2BR, α2CR and α2DR in goats. Our results suggest that the expression of the mRNAs and proteins of all three α2R subtypes are widely but unevenly distributed in the goat CNS and peripheral tissues. Furthermore, α2DR plays a more important role in α2R-mediated analgesia in goats than α2BR and α2CR, whereas α2CR activation exerts a greater effect on body temperature than α2BR and α2DR.