The First Case of Cytauxzoon spp. in Russia: The Parasite Conquers Eurasia

SIMPLE SUMMARY: Cytauxzoon felis was first described more than 40 years ago in the US, later (in the 21st century) similar pathogens were detected in Eurasian and Iberian lynxes, European wildcats and domestic cats in Southern and Central Europe. Our findings have shown the previously unrecorded presence of this parasite in Russia (50 km from Moscow). We described the crucial decrease in the number of leukocytes and erythrocytes, as well as in hemoglobin concentration, throughout the captive serval’s disease, and their increase during the animal’s recovery over six months. Molecular genetic methods allowed us to detect and describe this parasite in four cat species in captivity. The analysis showed high genetic variability and high occurrence of the parasites, which suggests their presence in free-ranging domestic cats and wild felids in Russia. ABSTRACT: Over the last two decades, Cytauxzoon spp. has been conquering Eurasia, although this fact has only been brought to light through recent more intensive research after the discovery of C. manul in Pallas’ cat. In Europe, Cytauxzoon was detected mainly in southern countries and later in central Europe. This pathogen has now been found in Russia for the first time (50 km from Moscow), this being the most northern sighting in Eurasia. A captive serval (Leptailurus serval) was found to be infected. Hematological analysis showed a crucial decrease in the number of leukocytes and erythrocytes, as well as in hemoglobin concentration. Genetic analysis confirmed the presence of Cytauxzoon spp. in serval blood at the beginning of the disease period. The identical pathogen was found in one bobcat at the same breeding center. Two other haplotypes of Cytauxzoon spp. were obtained from domestic cats at the same location, identical to the samples from Italy. One new haplotype, which was sequenced for the first time, was found in 7/7 investigated Amur wildcats (100%). The high occurrence and diversity of these pathogens suggest that they are present in free-ranging domestic cats and wild felids in Russia, and may be considered a potential threat to the endangered species. Current research shows that the genetic diversity of this pathogen may be even higher than it was suggested previously. Further genetic research is necessary to describe the diversity and phylogeny of this pathogen in Eurasia.