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C1q/TNF-Related Protein 3 Prevents Diabetic Retinopathy via AMPK-Dependent Stabilization of Blood–Retinal Barrier Tight Junctions

Background The impairment of the inner blood–retinal barrier (iBRB) increases the pathological development of diabetic retinopathy (DR), a severe complication in diabetic patients. Identifying approaches to preserving iBRB integrity and function is a significant challenge in DR. C1q/tumor necrosis f...

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Autores principales: Yan, Zheyi, Wang, Chunfang, Meng, Zhijun, Gan, Lu, Guo, Rui, Liu, Jing, Bond Lau, Wayne, Xie, Dina, Zhao, Jianli, Lopez, Bernard L., Christopher, Theodore A., Naik, Ulhas P., Ma, Xinliang, Wang, Yajing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8909652/
https://www.ncbi.nlm.nih.gov/pubmed/35269401
http://dx.doi.org/10.3390/cells11050779
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author Yan, Zheyi
Wang, Chunfang
Meng, Zhijun
Gan, Lu
Guo, Rui
Liu, Jing
Bond Lau, Wayne
Xie, Dina
Zhao, Jianli
Lopez, Bernard L.
Christopher, Theodore A.
Naik, Ulhas P.
Ma, Xinliang
Wang, Yajing
author_facet Yan, Zheyi
Wang, Chunfang
Meng, Zhijun
Gan, Lu
Guo, Rui
Liu, Jing
Bond Lau, Wayne
Xie, Dina
Zhao, Jianli
Lopez, Bernard L.
Christopher, Theodore A.
Naik, Ulhas P.
Ma, Xinliang
Wang, Yajing
author_sort Yan, Zheyi
collection PubMed
description Background The impairment of the inner blood–retinal barrier (iBRB) increases the pathological development of diabetic retinopathy (DR), a severe complication in diabetic patients. Identifying approaches to preserving iBRB integrity and function is a significant challenge in DR. C1q/tumor necrosis factor-related protein-3 (CTRP3) is a newly discovered adipokine and a vital biomarker, predicting DR severity. We sought to determine whether and how CTRP3 affects the pathological development of non-proliferative diabetic retinopathy (NPDR). Methods To clarify the pathophysiologic progress of the blood–retinal barrier in NPDR and explore its potential mechanism, a mouse Type 2 diabetic model of diabetic retinopathy was used. The capillary leakage was assessed by confocal microscope with fluorescent-labeled protein in vivo. Furthermore, the effect of CTRP3 on the inner blood–retinal barrier (iBRB) and its molecular mechanism was clarified. Results The results demonstrated that CTRP3 protects iBRB integrity and resists the vascular permeability induced by DR. Mechanistically, the administration of CTRP3 activates the AMPK signaling pathway and enhances the expression of Occludin and Claudin-5 (tight junction protein) in vivo and in vitro. Meanwhile, CTRP3 improves the injury of human retinal endothelial cells (HRMECs) induced by high glucose/high lipids (HG/HL), and its protective effects are AMPK-dependent. Conclusions In summary, we report, for the first time, that CTRP3 prevents diabetes-induced retinal vascular permeability via stabilizing the tight junctions of the iBRB and through the AMPK-dependent Occludin/Claudin-5 signaling pathway, thus critically affecting the development of NPDR.
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spelling pubmed-89096522022-03-11 C1q/TNF-Related Protein 3 Prevents Diabetic Retinopathy via AMPK-Dependent Stabilization of Blood–Retinal Barrier Tight Junctions Yan, Zheyi Wang, Chunfang Meng, Zhijun Gan, Lu Guo, Rui Liu, Jing Bond Lau, Wayne Xie, Dina Zhao, Jianli Lopez, Bernard L. Christopher, Theodore A. Naik, Ulhas P. Ma, Xinliang Wang, Yajing Cells Article Background The impairment of the inner blood–retinal barrier (iBRB) increases the pathological development of diabetic retinopathy (DR), a severe complication in diabetic patients. Identifying approaches to preserving iBRB integrity and function is a significant challenge in DR. C1q/tumor necrosis factor-related protein-3 (CTRP3) is a newly discovered adipokine and a vital biomarker, predicting DR severity. We sought to determine whether and how CTRP3 affects the pathological development of non-proliferative diabetic retinopathy (NPDR). Methods To clarify the pathophysiologic progress of the blood–retinal barrier in NPDR and explore its potential mechanism, a mouse Type 2 diabetic model of diabetic retinopathy was used. The capillary leakage was assessed by confocal microscope with fluorescent-labeled protein in vivo. Furthermore, the effect of CTRP3 on the inner blood–retinal barrier (iBRB) and its molecular mechanism was clarified. Results The results demonstrated that CTRP3 protects iBRB integrity and resists the vascular permeability induced by DR. Mechanistically, the administration of CTRP3 activates the AMPK signaling pathway and enhances the expression of Occludin and Claudin-5 (tight junction protein) in vivo and in vitro. Meanwhile, CTRP3 improves the injury of human retinal endothelial cells (HRMECs) induced by high glucose/high lipids (HG/HL), and its protective effects are AMPK-dependent. Conclusions In summary, we report, for the first time, that CTRP3 prevents diabetes-induced retinal vascular permeability via stabilizing the tight junctions of the iBRB and through the AMPK-dependent Occludin/Claudin-5 signaling pathway, thus critically affecting the development of NPDR. MDPI 2022-02-23 /pmc/articles/PMC8909652/ /pubmed/35269401 http://dx.doi.org/10.3390/cells11050779 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yan, Zheyi
Wang, Chunfang
Meng, Zhijun
Gan, Lu
Guo, Rui
Liu, Jing
Bond Lau, Wayne
Xie, Dina
Zhao, Jianli
Lopez, Bernard L.
Christopher, Theodore A.
Naik, Ulhas P.
Ma, Xinliang
Wang, Yajing
C1q/TNF-Related Protein 3 Prevents Diabetic Retinopathy via AMPK-Dependent Stabilization of Blood–Retinal Barrier Tight Junctions
title C1q/TNF-Related Protein 3 Prevents Diabetic Retinopathy via AMPK-Dependent Stabilization of Blood–Retinal Barrier Tight Junctions
title_full C1q/TNF-Related Protein 3 Prevents Diabetic Retinopathy via AMPK-Dependent Stabilization of Blood–Retinal Barrier Tight Junctions
title_fullStr C1q/TNF-Related Protein 3 Prevents Diabetic Retinopathy via AMPK-Dependent Stabilization of Blood–Retinal Barrier Tight Junctions
title_full_unstemmed C1q/TNF-Related Protein 3 Prevents Diabetic Retinopathy via AMPK-Dependent Stabilization of Blood–Retinal Barrier Tight Junctions
title_short C1q/TNF-Related Protein 3 Prevents Diabetic Retinopathy via AMPK-Dependent Stabilization of Blood–Retinal Barrier Tight Junctions
title_sort c1q/tnf-related protein 3 prevents diabetic retinopathy via ampk-dependent stabilization of blood–retinal barrier tight junctions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8909652/
https://www.ncbi.nlm.nih.gov/pubmed/35269401
http://dx.doi.org/10.3390/cells11050779
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